| GRILLOVÁ, Linda, Tanika BAWA, Lenka PAŠTĚKOVÁ, Angele GAYET-AGERON, Kay NIESELT, Michal STROUHAL, Patrice SEDNAOUI, Tristan FERRY, Matthias CAVASSINI, Stephan LAUTENSCHLAGER, Fabrizio DUTLY, Marta PLA-DIAZ, Michael KRUTZEN, Fernando GONZALEZ-CANDELAS, Homayoun C. BAGHERI, David ŠMAJS, Natascha ARORA and Philipp P. BOSSHARD. Molecular characterization of Treponema pallidum subsp pallidum in Switzerland and France with a new multilocus sequence typing scheme. Plos one. San Francisco: Public Library of Science, 2018, vol. 13, No 7, p. 1-16. ISSN 1932-6203. Available from: https://dx.doi.org/10.1371/journal.pone.0200773. |
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@article{1454936,
author = {Grillová, Linda and Bawa, Tanika and Paštěková, Lenka and GayetandAgeron, Angele and Nieselt, Kay and Strouhal, Michal and Sednaoui, Patrice and Ferry, Tristan and Cavassini, Matthias and Lautenschlager, Stephan and Dutly, Fabrizio and PlaandDiaz, Marta and Krutzen, Michael and GonzalezandCandelas, Fernando and Bagheri, Homayoun C. and Šmajs, David and Arora, Natascha and Bosshard, Philipp P.},
article_location = {San Francisco},
article_number = {7},
doi = {http://dx.doi.org/10.1371/journal.pone.0200773},
keywords = {Treponema pallidum},
language = {eng},
issn = {1932-6203},
journal = {Plos one},
title = {Molecular characterization of Treponema pallidum subsp pallidum in Switzerland and France with a new multilocus sequence typing scheme},
volume = {13},
year = {2018}
}
TY - JOUR
ID - 1454936
AU - Grillová, Linda - Bawa, Tanika - Paštěková, Lenka - Gayet-Ageron, Angele - Nieselt, Kay - Strouhal, Michal - Sednaoui, Patrice - Ferry, Tristan - Cavassini, Matthias - Lautenschlager, Stephan - Dutly, Fabrizio - Pla-Diaz, Marta - Krutzen, Michael - Gonzalez-Candelas, Fernando - Bagheri, Homayoun C. - Šmajs, David - Arora, Natascha - Bosshard, Philipp P.
PY - 2018
TI - Molecular characterization of Treponema pallidum subsp pallidum in Switzerland and France with a new multilocus sequence typing scheme
JF - Plos one
VL - 13
IS - 7
SP - 1-16
EP - 1-16
PB - Public Library of Science
SN - 19326203
KW - Treponema pallidum
N2 - Syphilis is an important public health problem and an increasing incidence has been noted in recent years. Characterization of strain diversity through molecular data plays a critical role in the epidemiological understanding of this re-emergence. We here propose a new high-resolution multilocus sequence typing (MLST) scheme for Treponema pallidum subsp. pallidum (TPA). We analyzed 30 complete and draft TPA genomes obtained directly from clinical samples or from rabbit propagated strains to identify suitable typing loci and tested the new scheme on 120 clinical samples collected in Switzerland and France. Our analyses yielded three loci with high discriminatory power: TP0136, TP0548, and TP0705. Together with analysis of the 23S rRNA gene mutations for macrolide resistance, we propose these loci as MLST for TPA. Among clinical samples, 23 allelic profiles as well as a high percentage (80% samples) of macrolide resistance were revealed. The new MLST has higher discriminatory power compared to previous typing schemes, enabling distinction of TPA from other treponemal bacteria, distinction between the two main TPA clades (Nichols and SS14), and differentiation of strains within these clades.
ER -
GRILLOVÁ, Linda, Tanika BAWA, Lenka PAŠTĚKOVÁ, Angele GAYET-AGERON, Kay NIESELT, Michal STROUHAL, Patrice SEDNAOUI, Tristan FERRY, Matthias CAVASSINI, Stephan LAUTENSCHLAGER, Fabrizio DUTLY, Marta PLA-DIAZ, Michael KRUTZEN, Fernando GONZALEZ-CANDELAS, Homayoun C. BAGHERI, David ŠMAJS, Natascha ARORA and Philipp P. BOSSHARD. Molecular characterization of Treponema pallidum subsp pallidum in Switzerland and France with a new multilocus sequence typing scheme. \textit{Plos one}. San Francisco: Public Library of Science, 2018, vol.~13, No~7, p.~1-16. ISSN~1932-6203. Available from: https://dx.doi.org/10.1371/journal.pone.0200773.
|
