Molecular characterization of Treponema pallidum subsp pallidum
in Switzerland and France with a new multilocus sequence typing
scheme

J 2018

Molecular characterization of Treponema pallidum subsp pallidum in Switzerland and France with a new multilocus sequence typing scheme

GRILLOVÁ, Linda, Tanika BAWA, Lenka PAŠTĚKOVÁ, Angele GAYET-AGERON, Kay NIESELT et. al.

Basic information

Original name

Molecular characterization of Treponema pallidum subsp pallidum in Switzerland and France with a new multilocus sequence typing scheme

Authors

GRILLOVÁ, Linda (203 Czech Republic, belonging to the institution), Tanika BAWA (756 Switzerland), Lenka PAŠTĚKOVÁ (203 Czech Republic, belonging to the institution), Angele GAYET-AGERON (756 Switzerland), Kay NIESELT (276 Germany), Michal STROUHAL (203 Czech Republic, belonging to the institution), Patrice SEDNAOUI (250 France), Tristan FERRY (250 France), Matthias CAVASSINI (756 Switzerland), Stephan LAUTENSCHLAGER (756 Switzerland), Fabrizio DUTLY (756 Switzerland), Marta PLA-DIAZ (724 Spain), Michael KRUTZEN (756 Switzerland), Fernando GONZALEZ-CANDELAS (724 Spain), Homayoun C. BAGHERI (724 Spain), David ŠMAJS (203 Czech Republic, belonging to the institution), Natascha ARORA (756 Switzerland) and Philipp P. BOSSHARD (756 Switzerland, guarantor)

Edition

Plos one, San Francisco, Public Library of Science, 2018, 1932-6203

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10601 Cell biology

Country of publisher

United States of America

Confidentiality degree

není předmětem státního či obchodního tajemství

Impact factor

Impact factor: 2.776

RIV identification code

RIV/00216224:14110/18:00101284

Organization unit

Faculty of Medicine

DOI

http://dx.doi.org/10.1371/journal.pone.0200773

UT WoS

000440251600016

Keywords in English

Treponema pallidum

Abstract

V originále

Syphilis is an important public health problem and an increasing incidence has been noted in recent years. Characterization of strain diversity through molecular data plays a critical role in the epidemiological understanding of this re-emergence. We here propose a new high-resolution multilocus sequence typing (MLST) scheme for Treponema pallidum subsp. pallidum (TPA). We analyzed 30 complete and draft TPA genomes obtained directly from clinical samples or from rabbit propagated strains to identify suitable typing loci and tested the new scheme on 120 clinical samples collected in Switzerland and France. Our analyses yielded three loci with high discriminatory power: TP0136, TP0548, and TP0705. Together with analysis of the 23S rRNA gene mutations for macrolide resistance, we propose these loci as MLST for TPA. Among clinical samples, 23 allelic profiles as well as a high percentage (80% samples) of macrolide resistance were revealed. The new MLST has higher discriminatory power compared to previous typing schemes, enabling distinction of TPA from other treponemal bacteria, distinction between the two main TPA clades (Nichols and SS14), and differentiation of strains within these clades.

Links

GA17-25455S, research and development project

Name: Studium genomů patogenních treponem na základě analýzy jednotlivých buněk

Investor: Czech Science Foundation

GJ17-25589Y, research and development project

Name: Kmeny způsobující syfilis: jsou geneticky odlišné kmeny odlišné i v průběhu experimentální infekce?

Investor: Czech Science Foundation

NV17-31333A, research and development project

Name: Vývoj nového typovacího systému pro původce syfilis, Treponema pallidum subsp. pallidum, zaměřeného na proteomické rozdíly

Citovat

GRILLOVÁ, Linda, Tanika BAWA, Lenka PAŠTĚKOVÁ, Angele GAYET-AGERON, Kay NIESELT, Michal STROUHAL, Patrice SEDNAOUI, Tristan FERRY, Matthias CAVASSINI, Stephan LAUTENSCHLAGER, Fabrizio DUTLY, Marta PLA-DIAZ, Michael KRUTZEN, Fernando GONZALEZ-CANDELAS, Homayoun C. BAGHERI, David ŠMAJS, Natascha ARORA and Philipp P. BOSSHARD. Molecular characterization of Treponema pallidum subsp pallidum in Switzerland and France with a new multilocus sequence typing scheme. Plos one. San Francisco: Public Library of Science, 2018, vol. 13, No 7, p. 1-16. ISSN 1932-6203. Available from: https://dx.doi.org/10.1371/journal.pone.0200773.

@article{1454936,
   author = {Grillová, Linda and Bawa, Tanika and Paštěková, Lenka and GayetandAgeron, Angele and Nieselt, Kay and Strouhal, Michal and Sednaoui, Patrice and Ferry, Tristan and Cavassini, Matthias and Lautenschlager, Stephan and Dutly, Fabrizio and PlaandDiaz, Marta and Krutzen, Michael and GonzalezandCandelas, Fernando and Bagheri, Homayoun C. and Šmajs, David and Arora, Natascha and Bosshard, Philipp P.},
   article_location = {San Francisco},
   article_number = {7},
   doi = {http://dx.doi.org/10.1371/journal.pone.0200773},
   keywords = {Treponema pallidum},
   language = {eng},
   issn = {1932-6203},
   journal = {Plos one},
   title = {Molecular characterization of Treponema pallidum subsp pallidum in Switzerland and France with a new multilocus sequence typing scheme},
   volume = {13},
   year = {2018}
}

TY  - JOUR
ID  - 1454936
AU  - Grillová, Linda - Bawa, Tanika - Paštěková, Lenka - Gayet-Ageron, Angele - Nieselt, Kay - Strouhal, Michal - Sednaoui, Patrice - Ferry, Tristan - Cavassini, Matthias - Lautenschlager, Stephan - Dutly, Fabrizio - Pla-Diaz, Marta - Krutzen, Michael - Gonzalez-Candelas, Fernando - Bagheri, Homayoun C. - Šmajs, David - Arora, Natascha - Bosshard, Philipp P.
PY  - 2018
TI  - Molecular characterization of Treponema pallidum subsp pallidum in Switzerland and France with a new multilocus sequence typing scheme
JF  - Plos one
VL  - 13
IS  - 7
SP  - 1-16
EP  - 1-16
PB  - Public Library of Science
SN  - 19326203
KW  - Treponema pallidum
N2  - Syphilis is an important public health problem and an increasing incidence has been noted in recent years. Characterization of strain diversity through molecular data plays a critical role in the epidemiological understanding of this re-emergence. We here propose a new high-resolution multilocus sequence typing (MLST) scheme for Treponema pallidum subsp. pallidum (TPA). We analyzed 30 complete and draft TPA genomes obtained directly from clinical samples or from rabbit propagated strains to identify suitable typing loci and tested the new scheme on 120 clinical samples collected in Switzerland and France. Our analyses yielded three loci with high discriminatory power: TP0136, TP0548, and TP0705. Together with analysis of the 23S rRNA gene mutations for macrolide resistance, we propose these loci as MLST for TPA. Among clinical samples, 23 allelic profiles as well as a high percentage (80% samples) of macrolide resistance were revealed. The new MLST has higher discriminatory power compared to previous typing schemes, enabling distinction of TPA from other treponemal bacteria, distinction between the two main TPA clades (Nichols and SS14), and differentiation of strains within these clades.
ER  -

GRILLOVÁ, Linda, Tanika BAWA, Lenka PAŠTĚKOVÁ, Angele GAYET-AGERON, Kay NIESELT, Michal STROUHAL, Patrice SEDNAOUI, Tristan FERRY, Matthias CAVASSINI, Stephan LAUTENSCHLAGER, Fabrizio DUTLY, Marta PLA-DIAZ, Michael KRUTZEN, Fernando GONZALEZ-CANDELAS, Homayoun C. BAGHERI, David ŠMAJS, Natascha ARORA and Philipp P. BOSSHARD. Molecular characterization of Treponema pallidum subsp pallidum in Switzerland and France with a new multilocus sequence typing scheme. \textit{Plos one}. San Francisco: Public Library of Science, 2018, vol.~13, No~7, p.~1-16. ISSN~1932-6203. Available from: https://dx.doi.org/10.1371/journal.pone.0200773.

Detailed Information on Publication Record