Absence of Surface IgD Does Not Impair Naive B Cell Homeostasis
or Memory B Cell Formation in IGHD Haploinsufficient Humans

J 2018

Absence of Surface IgD Does Not Impair Naive B Cell Homeostasis or Memory B Cell Formation in IGHD Haploinsufficient Humans

NECHVÁTALOVÁ, Jana, Sophinus J. W. BARTOL, Zita CHOVANCOVÁ, Louis BOON, Marcela VLKOVÁ et. al.

Basic information

Original name

Absence of Surface IgD Does Not Impair Naive B Cell Homeostasis or Memory B Cell Formation in IGHD Haploinsufficient Humans

Authors

NECHVÁTALOVÁ, Jana (203 Czech Republic, belonging to the institution), Sophinus J. W. BARTOL (528 Netherlands), Zita CHOVANCOVÁ (203 Czech Republic, belonging to the institution), Louis BOON (528 Netherlands), Marcela VLKOVÁ (203 Czech Republic, belonging to the institution) and Menno C. van ZELM (528 Netherlands, guarantor)

Edition

Journal of immunology, Bethesda, American association of immunologists, 2018, 0022-1767

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

30102 Immunology

Country of publisher

United States of America

Confidentiality degree

není předmětem státního či obchodního tajemství

Impact factor

Impact factor: 4.718

RIV identification code

RIV/00216224:14110/18:00106940

Organization unit

Faculty of Medicine

DOI

http://dx.doi.org/10.4049/jimmunol.1800767

UT WoS

000444804300014

Keywords in English

Surface IgD

Abstract

V originále

Surface IgD is coexpressed with IgM on naive mature B cells. Still, the role of surface IgD remains enigmatic even 50 y after its initial discovery. In this study, we examined the in vivo role of surface IgD in human B cell homeostasis and Ab responses in four individuals with heterozygous nonsense mutations in IGHD. All IGHD heterozygous individuals had normal numbers of B cells and serum Igs and did not show signs of immunodeficiency or immune dysregulation. IgD(+) and IgD(-) naive mature B cells were present in equal numbers and showed similar immunophenotypes, except for decreased expression of CD79b in the IgD(-) subset. Furthermore, both IgD(+) and IgD(-) naive mature B cells had normal replication histories and similar capacities to differentiate into plasma cells upon in vitro stimulation, and Ig class-switched memory B cells showed similar levels of somatic hypermutations. Thus, human B cells lacking IgD expression develop normally and generate immunological memory in vivo, suggesting that surface IgD might function more restrictedly in regulating of B cell activation to specific antigenic structures.

Links

NV15-28732A, research and development project

Name: Vliv granulocytů a monocytů na vznik a rozvoj imunodeficitních chorob a dalších imunopatologických dějů

Citovat

NECHVÁTALOVÁ, Jana, Sophinus J. W. BARTOL, Zita CHOVANCOVÁ, Louis BOON, Marcela VLKOVÁ and Menno C. van ZELM. Absence of Surface IgD Does Not Impair Naive B Cell Homeostasis or Memory B Cell Formation in IGHD Haploinsufficient Humans. Journal of immunology. Bethesda: American association of immunologists, 2018, vol. 201, No 7, p. 1928-1935. ISSN 0022-1767. Available from: https://dx.doi.org/10.4049/jimmunol.1800767.

@article{1455936,
   author = {Nechvátalová, Jana and Bartol, Sophinus J. W. and Chovancová, Zita and Boon, Louis and Vlková, Marcela and Zelm, Menno C. van},
   article_location = {Bethesda},
   article_number = {7},
   doi = {http://dx.doi.org/10.4049/jimmunol.1800767},
   keywords = {Surface IgD},
   language = {eng},
   issn = {0022-1767},
   journal = {Journal of immunology},
   title = {Absence of Surface IgD Does Not Impair Naive B Cell Homeostasis or Memory B Cell Formation in IGHD Haploinsufficient Humans},
   volume = {201},
   year = {2018}
}

TY  - JOUR
ID  - 1455936
AU  - Nechvátalová, Jana - Bartol, Sophinus J. W. - Chovancová, Zita - Boon, Louis - Vlková, Marcela - Zelm, Menno C. van
PY  - 2018
TI  - Absence of Surface IgD Does Not Impair Naive B Cell Homeostasis or Memory B Cell Formation in IGHD Haploinsufficient Humans
JF  - Journal of immunology
VL  - 201
IS  - 7
SP  - 1928-1935
EP  - 1928-1935
PB  - American association of immunologists
SN  - 00221767
KW  - Surface IgD
N2  - Surface IgD is coexpressed with IgM on naive mature B cells. Still, the role of surface IgD remains enigmatic even 50 y after its initial discovery. In this study, we examined the in vivo role of surface IgD in human B cell homeostasis and Ab responses in four individuals with heterozygous nonsense mutations in IGHD. All IGHD heterozygous individuals had normal numbers of B cells and serum Igs and did not show signs of immunodeficiency or immune dysregulation. IgD(+) and IgD(-) naive mature B cells were present in equal numbers and showed similar immunophenotypes, except for decreased expression of CD79b in the IgD(-) subset. Furthermore, both IgD(+) and IgD(-) naive mature B cells had normal replication histories and similar capacities to differentiate into plasma cells upon in vitro stimulation, and Ig class-switched memory B cells showed similar levels of somatic hypermutations. Thus, human B cells lacking IgD expression develop normally and generate immunological memory in vivo, suggesting that surface IgD might function more restrictedly in regulating of B cell activation to specific antigenic structures.
ER  -

NECHVÁTALOVÁ, Jana, Sophinus J. W. BARTOL, Zita CHOVANCOVÁ, Louis BOON, Marcela VLKOVÁ and Menno C. van ZELM. Absence of Surface IgD Does Not Impair Naive B Cell Homeostasis or Memory B Cell Formation in IGHD Haploinsufficient Humans. \textit{Journal of immunology}. Bethesda: American association of immunologists, 2018, vol.~201, No~7, p.~1928-1935. ISSN~0022-1767. Available from: https://dx.doi.org/10.4049/jimmunol.1800767.

Detailed Information on Publication Record