Presence of growth/differentiation factor-15 cytokine in human
follicular fluid, granulosa cells, and oocytes

J 2018

Presence of growth/differentiation factor-15 cytokine in human follicular fluid, granulosa cells, and oocytes

SOUČEK, Karel, Alice MALENOVSKÁ, Zuzana KAHOUNOVÁ, Ján REMŠÍK, Zuzana HOLUBCOVÁ et. al.

Basic information

Original name

Presence of growth/differentiation factor-15 cytokine in human follicular fluid, granulosa cells, and oocytes

Authors

SOUČEK, Karel (203 Czech Republic), Alice MALENOVSKÁ (203 Czech Republic), Zuzana KAHOUNOVÁ (203 Czech Republic), Ján REMŠÍK (703 Slovakia, belonging to the institution), Zuzana HOLUBCOVÁ (203 Czech Republic, belonging to the institution), Tomáš SOUKUP (203 Czech Republic), Daniela KURFÜRSTOVÁ (203 Czech Republic), Jan BOUCHAL (203 Czech Republic), Tereza SUCHANKOVÁ (203 Czech Republic), Eva SLABÁKOVÁ (203 Czech Republic) and Aleš HAMPL (203 Czech Republic, guarantor, belonging to the institution)

Edition

Journal of Assisted Reproduction and Genetics, NEW YORK, SPRINGER/PLENUM PUBLISHERS, 2018, 1058-0468

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10604 Reproductive biology

Country of publisher

United States of America

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

Full Text

Impact factor

Impact factor: 2.820

RIV identification code

RIV/00216224:14110/18:00101318

Organization unit

Faculty of Medicine

DOI

http://dx.doi.org/10.1007/s10815-018-1230-5

UT WoS

000441294700009

Keywords in English

Follicular fluid; Growth/differentiation factor-15; Follicular granulosa cells; IVF

Abstract

V originále

The purpose of the study was to determine whether the GDF-15 is present in follicular fluid; to evaluate if there is a relation between follicular and serum levels of GDF-15 and fertility status of study subjects; and to test whether granulosa cells, oocytes, or both produce GDF-15. This study used follicular fluid (FF, serum, and oocytes obtained under informed consent from women undergoing oocyte retrieval for in vitro fertilization. It also used ovaries from deceased preterm newborns. Collection of FF and blood at the time of oocyte retrieval, ELISA and western blot were performed to determine levels and forms of GDF-15. Concentrations of GDF-15 in FF and serum, its expression in ovarian tissue, and secretion from granulosa cells were analyzed. GDF-15 concentration in FF ranged from 35 to 572 ng/ml, as determined by ELISA. Western blot analysis revealed the GDF-15 pro-dimer only in FF. Both normal healthy and cancerous granulosa cells secreted GDF-15 into culture media. Primary oocytes displayed cytoplasmic GDF-15 positivity in immunostained newborn ovaries, and its expression was also observed in fully grown human oocytes. To the best of our knowledge, this is the first documentation of cytokine GDF-15 presence in follicular fluid. Its concentration was not associated with donor/patient fertility status. Our data also show that GDF-15 is expressed and inducible in both normal healthy and cancerous granulosa cells, as well as in oocytes.

Links

EE2.3.20.0185, research and development project

Name: Centrum analýz a modelování tkání a orgánů

GA15-11707S, research and development project

Name: Centrosomální abnormality u lidských pluripotentních kmenových buněk.

Investor: Czech Science Foundation

NV15-33999A, research and development project

Name: Vývoj nových nízkomolekulárních protinádorových léčiv na principu syntetické letality

Citovat

SOUČEK, Karel, Alice MALENOVSKÁ, Zuzana KAHOUNOVÁ, Ján REMŠÍK, Zuzana HOLUBCOVÁ, Tomáš SOUKUP, Daniela KURFÜRSTOVÁ, Jan BOUCHAL, Tereza SUCHANKOVÁ, Eva SLABÁKOVÁ and Aleš HAMPL. Presence of growth/differentiation factor-15 cytokine in human follicular fluid, granulosa cells, and oocytes. Journal of Assisted Reproduction and Genetics. NEW YORK: SPRINGER/PLENUM PUBLISHERS, 2018, vol. 35, No 8, p. 1407-1417. ISSN 1058-0468. Available from: https://dx.doi.org/10.1007/s10815-018-1230-5.

@article{1461056,
   author = {Souček, Karel and Malenovská, Alice and Kahounová, Zuzana and Remšík, Ján and Holubcová, Zuzana and Soukup, Tomáš and Kurfürstová, Daniela and Bouchal, Jan and Suchanková, Tereza and Slabáková, Eva and Hampl, Aleš},
   article_location = {NEW YORK},
   article_number = {8},
   doi = {http://dx.doi.org/10.1007/s10815-018-1230-5},
   keywords = {Follicular fluid; Growth/differentiation factor-15; Follicular granulosa cells; IVF},
   language = {eng},
   issn = {1058-0468},
   journal = {Journal of Assisted Reproduction and Genetics},
   title = {Presence of growth/differentiation factor-15 cytokine in human follicular fluid, granulosa cells, and oocytes},
   url = {https://link.springer.com/content/pdf/10.1007%2Fs10815-018-1230-5.pdf},
   volume = {35},
   year = {2018}
}

TY  - JOUR
ID  - 1461056
AU  - Souček, Karel - Malenovská, Alice - Kahounová, Zuzana - Remšík, Ján - Holubcová, Zuzana - Soukup, Tomáš - Kurfürstová, Daniela - Bouchal, Jan - Suchanková, Tereza - Slabáková, Eva - Hampl, Aleš
PY  - 2018
TI  - Presence of growth/differentiation factor-15 cytokine in human follicular fluid, granulosa cells, and oocytes
JF  - Journal of Assisted Reproduction and Genetics
VL  - 35
IS  - 8
SP  - 1407-1417
EP  - 1407-1417
PB  - SPRINGER/PLENUM PUBLISHERS
SN  - 10580468
KW  - Follicular fluid
KW  - Growth/differentiation factor-15
KW  - Follicular granulosa cells
KW  - IVF
UR  - https://link.springer.com/content/pdf/10.1007%2Fs10815-018-1230-5.pdf
L2  - https://link.springer.com/content/pdf/10.1007%2Fs10815-018-1230-5.pdf
N2  - The purpose of the study was to determine whether the GDF-15 is present in follicular fluid; to evaluate if there is a relation between follicular and serum levels of GDF-15 and fertility status of study subjects; and to test whether granulosa cells, oocytes, or both produce GDF-15. This study used follicular fluid (FF, serum, and oocytes obtained under informed consent from women undergoing oocyte retrieval for in vitro fertilization. It also used ovaries from deceased preterm newborns. Collection of FF and blood at the time of oocyte retrieval, ELISA and western blot were performed to determine levels and forms of GDF-15. Concentrations of GDF-15 in FF and serum, its expression in ovarian tissue, and secretion from granulosa cells were analyzed. GDF-15 concentration in FF ranged from 35 to 572 ng/ml, as determined by ELISA. Western blot analysis revealed the GDF-15 pro-dimer only in FF. Both normal healthy and cancerous granulosa cells secreted GDF-15 into culture media. Primary oocytes displayed cytoplasmic GDF-15 positivity in immunostained newborn ovaries, and its expression was also observed in fully grown human oocytes. To the best of our knowledge, this is the first documentation of cytokine GDF-15 presence in follicular fluid. Its concentration was not associated with donor/patient fertility status. Our data also show that GDF-15 is expressed and inducible in both normal healthy and cancerous granulosa cells, as well as in oocytes.
ER  -

SOUČEK, Karel, Alice MALENOVSKÁ, Zuzana KAHOUNOVÁ, Ján REMŠÍK, Zuzana HOLUBCOVÁ, Tomáš SOUKUP, Daniela KURFÜRSTOVÁ, Jan BOUCHAL, Tereza SUCHANKOVÁ, Eva SLABÁKOVÁ and Aleš HAMPL. Presence of growth/differentiation factor-15 cytokine in human follicular fluid, granulosa cells, and oocytes. \textit{Journal of Assisted Reproduction and Genetics}. NEW YORK: SPRINGER/PLENUM PUBLISHERS, 2018, vol.~35, No~8, p.~1407-1417. ISSN~1058-0468. Available from: https://dx.doi.org/10.1007/s10815-018-1230-5.

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