| GRILLOVÁ, Linda, Lorenzo GIACANI, Lenka PAŠTĚKOVÁ, Michal STROUHAL, Radim STRNADEL, Christina MARRA, Arturo CENTURION-LARA, Lucy POVEDA, Giancarlo RUSSO, Darina ČEJKOVÁ, Vladimír VAŠKŮ, Jan OPPELT and David ŠMAJS. Sequencing of Treponema pallidum subsp pallidum from isolate UZ1974 using Anti-Treponemal Antibodies Enrichment: First complete whole genome sequence obtained directly from human clinical material. Plos one. San Francisco: Public Library Science, 2018, vol. 13, No 8, p. 1-14. ISSN 1932-6203. Available from: https://dx.doi.org/10.1371/journal.pone.0202619. |
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@article{1461497,
author = {Grillová, Linda and Giacani, Lorenzo and Paštěková, Lenka and Strouhal, Michal and Strnadel, Radim and Marra, Christina and CenturionandLara, Arturo and Poveda, Lucy and Russo, Giancarlo and Čejková, Darina and Vašků, Vladimír and Oppelt, Jan and Šmajs, David},
article_location = {San Francisco},
article_number = {8},
doi = {http://dx.doi.org/10.1371/journal.pone.0202619},
keywords = {Treponema pallidum},
language = {eng},
issn = {1932-6203},
journal = {Plos one},
title = {Sequencing of Treponema pallidum subsp pallidum from isolate UZ1974 using Anti-Treponemal Antibodies Enrichment: First complete whole genome sequence obtained directly from human clinical material},
volume = {13},
year = {2018}
}
TY - JOUR
ID - 1461497
AU - Grillová, Linda - Giacani, Lorenzo - Paštěková, Lenka - Strouhal, Michal - Strnadel, Radim - Marra, Christina - Centurion-Lara, Arturo - Poveda, Lucy - Russo, Giancarlo - Čejková, Darina - Vašků, Vladimír - Oppelt, Jan - Šmajs, David
PY - 2018
TI - Sequencing of Treponema pallidum subsp pallidum from isolate UZ1974 using Anti-Treponemal Antibodies Enrichment: First complete whole genome sequence obtained directly from human clinical material
JF - Plos one
VL - 13
IS - 8
SP - 1-14
EP - 1-14
PB - Public Library Science
SN - 19326203
KW - Treponema pallidum
N2 - Treponema pallidum subsp. pallidum (TPA) is the infectious agent of syphilis, a disease that infects more than 5 million people annually. Since TPA is an uncultivable bacterium, most of the information on TPA genetics comes from genome sequencing and molecular typing studies. This study presents the first complete TPA genome (without sequencing gaps) of clinical isolate (UZ1974), which was obtained directly from clinical material, without multiplication in rabbits. Whole genome sequencing was performed using a newly developed Anti-Treponemal Antibody Enrichment technique combined with previously reported Pooled Segment Genome Sequencing. We identified the UW074B genome, isolated from a sample previously propagated in rabbits, to be the closest relative of the UZ1974 genome and calculated the TPA mutation rate as 2.8 x 10(-10) per site per generation.
ER -
GRILLOVÁ, Linda, Lorenzo GIACANI, Lenka PAŠTĚKOVÁ, Michal STROUHAL, Radim STRNADEL, Christina MARRA, Arturo CENTURION-LARA, Lucy POVEDA, Giancarlo RUSSO, Darina ČEJKOVÁ, Vladimír VAŠKŮ, Jan OPPELT and David ŠMAJS. Sequencing of Treponema pallidum subsp pallidum from isolate UZ1974 using Anti-Treponemal Antibodies Enrichment: First complete whole genome sequence obtained directly from human clinical material. \textit{Plos one}. San Francisco: Public Library Science, 2018, vol.~13, No~8, p.~1-14. ISSN~1932-6203. Available from: https://dx.doi.org/10.1371/journal.pone.0202619.
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