Sequencing of Treponema pallidum subsp pallidum from isolate
UZ1974 using Anti-Treponemal Antibodies Enrichment: First
complete whole genome sequence obtained directly from human
clinical material

J 2018

Sequencing of Treponema pallidum subsp pallidum from isolate UZ1974 using Anti-Treponemal Antibodies Enrichment: First complete whole genome sequence obtained directly from human clinical material

GRILLOVÁ, Linda, Lorenzo GIACANI, Lenka PAŠTĚKOVÁ, Michal STROUHAL, Radim STRNADEL et. al.

Basic information

Original name

Sequencing of Treponema pallidum subsp pallidum from isolate UZ1974 using Anti-Treponemal Antibodies Enrichment: First complete whole genome sequence obtained directly from human clinical material

Authors

GRILLOVÁ, Linda (203 Czech Republic, belonging to the institution), Lorenzo GIACANI (840 United States of America), Lenka PAŠTĚKOVÁ (203 Czech Republic, belonging to the institution), Michal STROUHAL (203 Czech Republic, belonging to the institution), Radim STRNADEL (203 Czech Republic), Christina MARRA (840 United States of America), Arturo CENTURION-LARA (840 United States of America), Lucy POVEDA (756 Switzerland), Giancarlo RUSSO (756 Switzerland), Darina ČEJKOVÁ (203 Czech Republic), Vladimír VAŠKŮ (203 Czech Republic, belonging to the institution), Jan OPPELT (203 Czech Republic, belonging to the institution) and David ŠMAJS (203 Czech Republic, guarantor, belonging to the institution)

Edition

Plos one, San Francisco, Public Library Science, 2018, 1932-6203

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10601 Cell biology

Country of publisher

United States of America

Confidentiality degree

není předmětem státního či obchodního tajemství

Impact factor

Impact factor: 2.776

RIV identification code

RIV/00216224:14110/18:00101320

Organization unit

Faculty of Medicine

DOI

http://dx.doi.org/10.1371/journal.pone.0202619

UT WoS

000442284500011

Keywords in English

Treponema pallidum

Abstract

V originále

Treponema pallidum subsp. pallidum (TPA) is the infectious agent of syphilis, a disease that infects more than 5 million people annually. Since TPA is an uncultivable bacterium, most of the information on TPA genetics comes from genome sequencing and molecular typing studies. This study presents the first complete TPA genome (without sequencing gaps) of clinical isolate (UZ1974), which was obtained directly from clinical material, without multiplication in rabbits. Whole genome sequencing was performed using a newly developed Anti-Treponemal Antibody Enrichment technique combined with previously reported Pooled Segment Genome Sequencing. We identified the UW074B genome, isolated from a sample previously propagated in rabbits, to be the closest relative of the UZ1974 genome and calculated the TPA mutation rate as 2.8 x 10(-10) per site per generation.

Links

GA17-25455S, research and development project

Name: Studium genomů patogenních treponem na základě analýzy jednotlivých buněk

Investor: Czech Science Foundation

LM2015043, research and development project

Name: Česká infrastruktura pro integrativní strukturní biologii (Acronym: CIISB)

Investor: Ministry of Education, Youth and Sports of the CR

NV17-31333A, research and development project

Name: Vývoj nového typovacího systému pro původce syfilis, Treponema pallidum subsp. pallidum, zaměřeného na proteomické rozdíly

Citovat

GRILLOVÁ, Linda, Lorenzo GIACANI, Lenka PAŠTĚKOVÁ, Michal STROUHAL, Radim STRNADEL, Christina MARRA, Arturo CENTURION-LARA, Lucy POVEDA, Giancarlo RUSSO, Darina ČEJKOVÁ, Vladimír VAŠKŮ, Jan OPPELT and David ŠMAJS. Sequencing of Treponema pallidum subsp pallidum from isolate UZ1974 using Anti-Treponemal Antibodies Enrichment: First complete whole genome sequence obtained directly from human clinical material. Plos one. San Francisco: Public Library Science, 2018, vol. 13, No 8, p. 1-14. ISSN 1932-6203. Available from: https://dx.doi.org/10.1371/journal.pone.0202619.

@article{1461497,
   author = {Grillová, Linda and Giacani, Lorenzo and Paštěková, Lenka and Strouhal, Michal and Strnadel, Radim and Marra, Christina and CenturionandLara, Arturo and Poveda, Lucy and Russo, Giancarlo and Čejková, Darina and Vašků, Vladimír and Oppelt, Jan and Šmajs, David},
   article_location = {San Francisco},
   article_number = {8},
   doi = {http://dx.doi.org/10.1371/journal.pone.0202619},
   keywords = {Treponema pallidum},
   language = {eng},
   issn = {1932-6203},
   journal = {Plos one},
   title = {Sequencing of Treponema pallidum subsp pallidum from isolate UZ1974 using Anti-Treponemal Antibodies Enrichment: First complete whole genome sequence obtained directly from human clinical material},
   volume = {13},
   year = {2018}
}

TY  - JOUR
ID  - 1461497
AU  - Grillová, Linda - Giacani, Lorenzo - Paštěková, Lenka - Strouhal, Michal - Strnadel, Radim - Marra, Christina - Centurion-Lara, Arturo - Poveda, Lucy - Russo, Giancarlo - Čejková, Darina - Vašků, Vladimír - Oppelt, Jan - Šmajs, David
PY  - 2018
TI  - Sequencing of Treponema pallidum subsp pallidum from isolate UZ1974 using Anti-Treponemal Antibodies Enrichment: First complete whole genome sequence obtained directly from human clinical material
JF  - Plos one
VL  - 13
IS  - 8
SP  - 1-14
EP  - 1-14
PB  - Public Library Science
SN  - 19326203
KW  - Treponema pallidum
N2  - Treponema pallidum subsp. pallidum (TPA) is the infectious agent of syphilis, a disease that infects more than 5 million people annually. Since TPA is an uncultivable bacterium, most of the information on TPA genetics comes from genome sequencing and molecular typing studies. This study presents the first complete TPA genome (without sequencing gaps) of clinical isolate (UZ1974), which was obtained directly from clinical material, without multiplication in rabbits. Whole genome sequencing was performed using a newly developed Anti-Treponemal Antibody Enrichment technique combined with previously reported Pooled Segment Genome Sequencing. We identified the UW074B genome, isolated from a sample previously propagated in rabbits, to be the closest relative of the UZ1974 genome and calculated the TPA mutation rate as 2.8 x 10(-10) per site per generation.
ER  -

GRILLOVÁ, Linda, Lorenzo GIACANI, Lenka PAŠTĚKOVÁ, Michal STROUHAL, Radim STRNADEL, Christina MARRA, Arturo CENTURION-LARA, Lucy POVEDA, Giancarlo RUSSO, Darina ČEJKOVÁ, Vladimír VAŠKŮ, Jan OPPELT and David ŠMAJS. Sequencing of Treponema pallidum subsp pallidum from isolate UZ1974 using Anti-Treponemal Antibodies Enrichment: First complete whole genome sequence obtained directly from human clinical material. \textit{Plos one}. San Francisco: Public Library Science, 2018, vol.~13, No~8, p.~1-14. ISSN~1932-6203. Available from: https://dx.doi.org/10.1371/journal.pone.0202619.

Detailed Information on Publication Record