Escherichia coli isolates from patients with inflammatory bowel
disease: ExPEC virulence- and colicin-determinants are more
frequent compared to healthy controls

J 2018

Escherichia coli isolates from patients with inflammatory bowel disease: ExPEC virulence- and colicin-determinants are more frequent compared to healthy controls

MICENKOVÁ, Lenka, Lucia FRANKOVIČOVÁ, Iva JABORNÍKOVÁ, Juraj BOSÁK, Petr DÍTĚ et. al.

Basic information

Original name

Escherichia coli isolates from patients with inflammatory bowel disease: ExPEC virulence- and colicin-determinants are more frequent compared to healthy controls

Authors

MICENKOVÁ, Lenka (703 Slovakia, belonging to the institution), Lucia FRANKOVIČOVÁ (703 Slovakia), Iva JABORNÍKOVÁ (203 Czech Republic, belonging to the institution), Juraj BOSÁK (703 Slovakia, belonging to the institution), Petr DÍTĚ (203 Czech Republic, belonging to the institution), Jan ŠMARDA (203 Czech Republic, belonging to the institution), Martin VRBA (203 Czech Republic), Alena ŠEVČÍKOVÁ (203 Czech Republic), Marta KMEŤOVÁ (703 Slovakia) and David ŠMAJS (203 Czech Republic, guarantor, belonging to the institution)

Edition

International Journal of Medical Microbiology, JENA, Urban & Fischer Verlag, 2018, 1438-4221

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10606 Microbiology

Country of publisher

Germany

Confidentiality degree

není předmětem státního či obchodního tajemství

Impact factor

Impact factor: 3.362

RIV identification code

RIV/00216224:14110/18:00101321

Organization unit

Faculty of Medicine

DOI

http://dx.doi.org/10.1016/j.ijmm.2018.04.008

UT WoS

000440393900002

Keywords in English

E. coli; Crohn's disease; Ulcerative colitis; Virulence; Bacteriocin; Phylogenetic group

Abstract

V originále

A set of 178 Escherichia coli isolates taken from patients with inflammatory bowel disease (IBD) was analyzed for bacteriocin production and tested for the prevalence of 30 bacteriocin and 22 virulence factor determinants. Additionally, E. coli phylogenetic groups were also determined. Pulsed-field gel electrophoresis (PFGE) was used for exclusion of clonal character of isolates. Results were compared to data from a previously published analysis of 1283 fecal commensal E. coil isolates. The frequency of bacteriocinogenic isolates (66.9%) was significantly higher in IBD E. coli compared to fecal commensal E. coli isolates (54.2%, p < 0.01). In the group of IBD E. call isolates, a higher prevalence of determinants for group B colicins (i.e., colicins B, D, Ia, Ib, M, and 5/10) (p < 0.01), including a higher prevalence of the colicin B determinant (p < 0.01) was found. Virulence factor determinants encoding fimbriae (fin/A, 91.0%; pap, 27.5%), cytotoxic necrotizing factor (cnfl, 11.2%), aerobactin synthesis (aer, 43.3%), and the locus associated with invasivity (ial, 9.0%) were more prevalent in IBD E. coli (p < 0.05 for all five determinants). E. coli isolates from IBD mucosal biopsies were more frequently bacteriocinogenic (84.6%, p < 0.01) compared to fecal IBD isolates and fecal commensal E. coli. PFGE analysis revealed clusters specific for IBD E. coli isolates (n = 11), for fecal isolates (n = 13), and clusters containing both IBD and fecal isolates (n = 10). ExPEC (Extraintestinal Pathogenic E. coli) virulence and colicin determinants appear to be important characteristics of IBD E. coil isolates, especially the E. coli isolates obtained directly from biopsy samples.

Links

EF15_003/0000469, research and development project

Name: Cetocoen Plus

GA16-21649S, research and development project

Name: Molekulární charakterizace nových bakteriocinů identifikovaných v rodech Escherichia a Shigella

Investor: Czech Science Foundation

LM2015051, research and development project

Name: Centrum pro výzkum toxických látek v prostředí (Acronym: RECETOX RI)

Investor: Ministry of Education, Youth and Sports of the CR

ROZV/25/LF/2017, interní kód MU

Name: LF - Příspěvek na IP 2017

Investor: Ministry of Education, Youth and Sports of the CR, Internal development projects

Citovat

MICENKOVÁ, Lenka, Lucia FRANKOVIČOVÁ, Iva JABORNÍKOVÁ, Juraj BOSÁK, Petr DÍTĚ, Jan ŠMARDA, Martin VRBA, Alena ŠEVČÍKOVÁ, Marta KMEŤOVÁ and David ŠMAJS. Escherichia coli isolates from patients with inflammatory bowel disease: ExPEC virulence- and colicin-determinants are more frequent compared to healthy controls. International Journal of Medical Microbiology. JENA: Urban & Fischer Verlag, 2018, vol. 308, No 5, p. 498-504. ISSN 1438-4221. Available from: https://dx.doi.org/10.1016/j.ijmm.2018.04.008.

@article{1461537,
   author = {Micenková, Lenka and Frankovičová, Lucia and Jaborníková, Iva and Bosák, Juraj and Dítě, Petr and Šmarda, Jan and Vrba, Martin and Ševčíková, Alena and Kmeťová, Marta and Šmajs, David},
   article_location = {JENA},
   article_number = {5},
   doi = {http://dx.doi.org/10.1016/j.ijmm.2018.04.008},
   keywords = {E. coli; Crohn's disease; Ulcerative colitis; Virulence; Bacteriocin; Phylogenetic group},
   language = {eng},
   issn = {1438-4221},
   journal = {International Journal of Medical Microbiology},
   title = {Escherichia coli isolates from patients with inflammatory bowel disease: ExPEC virulence- and colicin-determinants are more frequent compared to healthy controls},
   volume = {308},
   year = {2018}
}

TY  - JOUR
ID  - 1461537
AU  - Micenková, Lenka - Frankovičová, Lucia - Jaborníková, Iva - Bosák, Juraj - Dítě, Petr - Šmarda, Jan - Vrba, Martin - Ševčíková, Alena - Kmeťová, Marta - Šmajs, David
PY  - 2018
TI  - Escherichia coli isolates from patients with inflammatory bowel disease: ExPEC virulence- and colicin-determinants are more frequent compared to healthy controls
JF  - International Journal of Medical Microbiology
VL  - 308
IS  - 5
SP  - 498-504
EP  - 498-504
PB  - Urban & Fischer Verlag
SN  - 14384221
KW  - E. coli
KW  - Crohn's disease
KW  - Ulcerative colitis
KW  - Virulence
KW  - Bacteriocin
KW  - Phylogenetic group
N2  - A set of 178 Escherichia coli isolates taken from patients with inflammatory bowel disease (IBD) was analyzed for bacteriocin production and tested for the prevalence of 30 bacteriocin and 22 virulence factor determinants. Additionally, E. coli phylogenetic groups were also determined. Pulsed-field gel electrophoresis (PFGE) was used for exclusion of clonal character of isolates. Results were compared to data from a previously published analysis of 1283 fecal commensal E. coil isolates. The frequency of bacteriocinogenic isolates (66.9%) was significantly higher in IBD E. coli compared to fecal commensal E. coli isolates (54.2%, p < 0.01). In the group of IBD E. call isolates, a higher prevalence of determinants for group B colicins (i.e., colicins B, D, Ia, Ib, M, and 5/10) (p < 0.01), including a higher prevalence of the colicin B determinant (p < 0.01) was found. Virulence factor determinants encoding fimbriae (fin/A, 91.0%; pap, 27.5%), cytotoxic necrotizing factor (cnfl, 11.2%), aerobactin synthesis (aer, 43.3%), and the locus associated with invasivity (ial, 9.0%) were more prevalent in IBD E. coli (p < 0.05 for all five determinants). E. coli isolates from IBD mucosal biopsies were more frequently bacteriocinogenic (84.6%, p < 0.01) compared to fecal IBD isolates and fecal commensal E. coli. PFGE analysis revealed clusters specific for IBD E. coli isolates (n = 11), for fecal isolates (n = 13), and clusters containing both IBD and fecal isolates (n = 10). ExPEC (Extraintestinal Pathogenic E. coli) virulence and colicin determinants appear to be important characteristics of IBD E. coil isolates, especially the E. coli isolates obtained directly from biopsy samples.
ER  -

MICENKOVÁ, Lenka, Lucia FRANKOVIČOVÁ, Iva JABORNÍKOVÁ, Juraj BOSÁK, Petr DÍTĚ, Jan ŠMARDA, Martin VRBA, Alena ŠEVČÍKOVÁ, Marta KMEŤOVÁ and David ŠMAJS. Escherichia coli isolates from patients with inflammatory bowel disease: ExPEC virulence- and colicin-determinants are more frequent compared to healthy controls. \textit{International Journal of Medical Microbiology}. JENA: Urban \&{} Fischer Verlag, 2018, vol.~308, No~5, p.~498-504. ISSN~1438-4221. Available from: https://dx.doi.org/10.1016/j.ijmm.2018.04.008.

Detailed Information on Publication Record