Irradiation by gamma-rays reduces the level of H3S10
phosphorylation and weakens the G2 phase-dependent interaction
between H3S10 phosphorylation and gamma H2AX

J 2018

Irradiation by gamma-rays reduces the level of H3S10 phosphorylation and weakens the G2 phase-dependent interaction between H3S10 phosphorylation and gamma H2AX

BÁRTOVÁ, Eva, Gabriela LOCHMANOVÁ, Soňa LEGARTOVÁ, Jana SUCHÁNKOVÁ, Radek FEDR et. al.

Basic information

Original name

Irradiation by gamma-rays reduces the level of H3S10 phosphorylation and weakens the G2 phase-dependent interaction between H3S10 phosphorylation and gamma H2AX

Authors

BÁRTOVÁ, Eva (203 Czech Republic), Gabriela LOCHMANOVÁ (203 Czech Republic, belonging to the institution), Soňa LEGARTOVÁ (203 Czech Republic), Jana SUCHÁNKOVÁ (203 Czech Republic), Radek FEDR (203 Czech Republic), Jana KREJČÍ (203 Czech Republic) and Zbyněk ZDRÁHAL (203 Czech Republic, guarantor, belonging to the institution)

Edition

Biochimie, Elsevier, 2018, 0300-9084

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10608 Biochemistry and molecular biology

Country of publisher

France

Confidentiality degree

není předmětem státního či obchodního tajemství

Impact factor

Impact factor: 3.362

RIV identification code

RIV/00216224:14740/18:00101337

Organization unit

Central European Institute of Technology

DOI

http://dx.doi.org/10.1016/j.biochi.2018.07.029

UT WoS

000448032300011

Keywords in English

Mass spectrometry; Histones H3 and H4; gamma H2AX; DNA damage; Embryonic stem cells; Epigenetics

Abstract

V originále

Histone posttranslational modifications regulate diverse nuclear functions, including DNA repair. Here, we use mass spectrometry, western blotting, immunohistochemistry and advanced confocal microscopy in order to show radiation-specific changes in the histone signature. We studied wild-type mouse embryonic stem cells (mESCs) and mESCs with a depletion of histone deacetylase 1 (HDAC1), which plays a role in DNA repair. Irradiation by gamma-rays increased the S139 phosphorylation of histone H2AX but reduced the level of the H3K9-R17 peptide, which contains S10 phosphorylation (H3S10ph). On an individual cellular level, H3S10ph was low in highly gamma H2AX-positive UV laser-induced DNA lesions, and this nuclear distribution pattern was not changed by HDAC1 depletion. Despite this fact, spontaneous gamma H2AX-positive DNA lesions colocalized with large H3S10ph-positive nuclear bodies that appear in the G2 phase of the cell cycle. Similarly, by FLIM-FRET analysis, we observed an interaction between H3S10ph and gamma H2AX in the G2 phase. However, this interaction was reduced when cells were exposed to gamma-rays. A mutual link between H3S10ph and gamma H2AX was not observed in the G1 phase of the cell cycle. Together, our data show that despite the fact that H3S10ph is not directly involved in DNA repair, a decrease in H3S10 phosphorylation and weakened interaction between H3S10ph and gamma H2AX is a result of radiation-induced damage of the genome. In this case, gamma-irradiation also decreased the number of cells in the G1 phase, characterized by no interaction between H3S10ph and gamma H2AX. (C) 2018 Elsevier B.V. and Societe Francaise de Biochimie et Biologie Moleculaire (SFBBM).

Links

GA18-07384S, research and development project

Name: Od konformace po biologické funkce proteinu HP1

Investor: Czech Science Foundation

LM2015043, research and development project

Name: Česká infrastruktura pro integrativní strukturní biologii (Acronym: CIISB)

Investor: Ministry of Education, Youth and Sports of the CR

LQ1601, research and development project

Name: CEITEC 2020 (Acronym: CEITEC2020)

Investor: Ministry of Education, Youth and Sports of the CR

Citovat

BÁRTOVÁ, Eva, Gabriela LOCHMANOVÁ, Soňa LEGARTOVÁ, Jana SUCHÁNKOVÁ, Radek FEDR, Jana KREJČÍ and Zbyněk ZDRÁHAL. Irradiation by gamma-rays reduces the level of H3S10 phosphorylation and weakens the G2 phase-dependent interaction between H3S10 phosphorylation and gamma H2AX. Biochimie. Elsevier, 2018, vol. 154, NOV, p. 86-98. ISSN 0300-9084. Available from: https://dx.doi.org/10.1016/j.biochi.2018.07.029.

@article{1466204,
   author = {Bártová, Eva and Lochmanová, Gabriela and Legartová, Soňa and Suchánková, Jana and Fedr, Radek and Krejčí, Jana and Zdráhal, Zbyněk},
   article_number = {NOV},
   doi = {http://dx.doi.org/10.1016/j.biochi.2018.07.029},
   keywords = {Mass spectrometry; Histones H3 and H4; gamma H2AX; DNA damage; Embryonic stem cells; Epigenetics},
   language = {eng},
   issn = {0300-9084},
   journal = {Biochimie},
   title = {Irradiation by gamma-rays reduces the level of H3S10 phosphorylation and weakens the G2 phase-dependent interaction between H3S10 phosphorylation and gamma H2AX},
   volume = {154},
   year = {2018}
}

TY  - JOUR
ID  - 1466204
AU  - Bártová, Eva - Lochmanová, Gabriela - Legartová, Soňa - Suchánková, Jana - Fedr, Radek - Krejčí, Jana - Zdráhal, Zbyněk
PY  - 2018
TI  - Irradiation by gamma-rays reduces the level of H3S10 phosphorylation and weakens the G2 phase-dependent interaction between H3S10 phosphorylation and gamma H2AX
JF  - Biochimie
VL  - 154
IS  - NOV
SP  - 86-98
EP  - 86-98
PB  - Elsevier
SN  - 03009084
KW  - Mass spectrometry
KW  - Histones H3 and H4
KW  - gamma H2AX
KW  - DNA damage
KW  - Embryonic stem cells
KW  - Epigenetics
N2  - Histone posttranslational modifications regulate diverse nuclear functions, including DNA repair. Here, we use mass spectrometry, western blotting, immunohistochemistry and advanced confocal microscopy in order to show radiation-specific changes in the histone signature. We studied wild-type mouse embryonic stem cells (mESCs) and mESCs with a depletion of histone deacetylase 1 (HDAC1), which plays a role in DNA repair. Irradiation by gamma-rays increased the S139 phosphorylation of histone H2AX but reduced the level of the H3K9-R17 peptide, which contains S10 phosphorylation (H3S10ph). On an individual cellular level, H3S10ph was low in highly gamma H2AX-positive UV laser-induced DNA lesions, and this nuclear distribution pattern was not changed by HDAC1 depletion. Despite this fact, spontaneous gamma H2AX-positive DNA lesions colocalized with large H3S10ph-positive nuclear bodies that appear in the G2 phase of the cell cycle. Similarly, by FLIM-FRET analysis, we observed an interaction between H3S10ph and gamma H2AX in the G2 phase. However, this interaction was reduced when cells were exposed to gamma-rays. A mutual link between H3S10ph and gamma H2AX was not observed in the G1 phase of the cell cycle. Together, our data show that despite the fact that H3S10ph is not directly involved in DNA repair, a decrease in H3S10 phosphorylation and weakened interaction between H3S10ph and gamma H2AX is a result of radiation-induced damage of the genome. In this case, gamma-irradiation also decreased the number of cells in the G1 phase, characterized by no interaction between H3S10ph and gamma H2AX. (C) 2018 Elsevier B.V. and Societe Francaise de Biochimie et Biologie Moleculaire (SFBBM).
ER  -

BÁRTOVÁ, Eva, Gabriela LOCHMANOVÁ, Soňa LEGARTOVÁ, Jana SUCHÁNKOVÁ, Radek FEDR, Jana KREJČÍ and Zbyněk ZDRÁHAL. Irradiation by gamma-rays reduces the level of H3S10 phosphorylation and weakens the G2 phase-dependent interaction between H3S10 phosphorylation and gamma H2AX. \textit{Biochimie}. Elsevier, 2018, vol.~154, NOV, p.~86-98. ISSN~0300-9084. Available from: https://dx.doi.org/10.1016/j.biochi.2018.07.029.

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