J 2018

Effect of jasplakinolide and cytochalasin D on cortical elements involved in the gliding motility of the eugregarine Gregarina garnhami (Apicomplexa)

KOVÁČIKOVÁ, Magdaléna, Naděžda VAŠKOVICOVÁ, Jana NEBESÁŘOVÁ and Andrea BARDŮNEK VALIGUROVÁ

Basic information

Original name

Effect of jasplakinolide and cytochalasin D on cortical elements involved in the gliding motility of the eugregarine Gregarina garnhami (Apicomplexa)

Authors

KOVÁČIKOVÁ, Magdaléna (703 Slovakia, guarantor, belonging to the institution), Naděžda VAŠKOVICOVÁ (203 Czech Republic), Jana NEBESÁŘOVÁ (203 Czech Republic) and Andrea BARDŮNEK VALIGUROVÁ (703 Slovakia, belonging to the institution)

Edition

European Journal of Protistology, JENA, ELSEVIER GMBH, 2018, 0932-4739

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10601 Cell biology

Country of publisher

Germany

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

Impact factor

Impact factor: 2.626

RIV identification code

RIV/00216224:14310/18:00104436

Organization unit

Faculty of Science

DOI

UT WoS

000452347600011

Keywords in English

Ectoplasmic network; F-actin; Gregarine; Motility; Myonemes; Ultrastructure

Tags

International impact, Reviewed
Změněno: 23/4/2024 12:40, Mgr. Michal Petr

Abstract

V originále

Since apicomplexans represent exclusively parasitic unicellular organisms with medical and economic impacts, the principles of their motility have been studied intensively. By contrast, the movement in apicomplexan basal groups, such as gregarines, remains to be elucidated. The present study focuses on Gregarina garnhami parasitising the digestive tract of the locust Schistocerca gregaria, and investigates the involvement of cytoskeletal elements (the ectoplasmic network and myonemes) and the secretion of mucosubstances during eugregarine gliding motility. Combined microscopic analyses were used to verify the role of actin filaments and membranes' organisation in G. garnhami motility. A freeze-etching analysis of membranes revealed the size, density, and arrangement of intramembranous particles along with the distribution and size of pores and ducts. Experimental assays using actin-modifying drugs (jasplakinolide, cytochalasin D) confirmed that actin most likely plays a role in cell motility, principally in its filamentous form (=F-actin). Myonemes, localised in the border between the ectoplasm and endoplasm, correspond to the concentric bundles of F-actin. Microscopic analyses confirmed that changes in gamonts motility corresponding to the changes in the organisation and density of myonemes and the ectoplasmic network in drug-treated cells, suggesting that these structures might serve as contractile elements facilitating gliding motility in G. garnhami.