| KADEŘÁVEK, Pavel, F. FERRAGE, G. BODENHAUSEN and D. KURZBACH. High-Resolution NMR of Folded Proteins in Hyperpolarized Physiological Solvents. Chemistry - A European Journal. WEINHEIM: Wiley, 2018, vol. 24, No 51, p. 13418-13423. ISSN 0947-6539. Available from: https://dx.doi.org/10.1002/chem.201802885. |
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@article{1468956,
author = {Kadeřávek, Pavel and Ferrage, F. and Bodenhausen, G. and Kurzbach, D.},
article_location = {WEINHEIM},
article_number = {51},
doi = {http://dx.doi.org/10.1002/chem.201802885},
keywords = {dissolution DNP; hyperpolarized water; cross-relaxation effect; protein NMR spectroscopy; proton exchange},
language = {eng},
issn = {0947-6539},
journal = {Chemistry - A European Journal},
title = {High-Resolution NMR of Folded Proteins in Hyperpolarized Physiological Solvents},
url = {https://onlinelibrary.wiley.com/doi/full/10.1002/chem.201802885},
volume = {24},
year = {2018}
}
TY - JOUR
ID - 1468956
AU - Kadeřávek, Pavel - Ferrage, F. - Bodenhausen, G. - Kurzbach, D.
PY - 2018
TI - High-Resolution NMR of Folded Proteins in Hyperpolarized Physiological Solvents
JF - Chemistry - A European Journal
VL - 24
IS - 51
SP - 13418-13423
EP - 13418-13423
PB - Wiley
SN - 09476539
KW - dissolution DNP
KW - hyperpolarized water
KW - cross-relaxation effect
KW - protein NMR spectroscopy
KW - proton exchange
UR - https://onlinelibrary.wiley.com/doi/full/10.1002/chem.201802885
N2 - Hyperpolarized 2D exchange spectroscopy (HYPEX) to obtain high-resolution nuclear magnetic resonance (NMR) spectra of folded proteins under near-physiological conditions is reported. The technique is based on hyperpolarized water, which is prepared by dissolution dynamic nuclear polarization and mixed in situ in an NMR spectrometer with a protein in a physiological saline buffer at body temperature. Rapid exchange of labile protons with the hyperpolarized solvent, combined with cross-relaxation effects (NOEs), leads to boosted signal intensities for many amide H-1-N-15 correlations in the protein ubiquitin. As the introduction of hyperpolarization to the target protein is mediated via the solvent, the method is applicable to a broad spectrum of target molecules.
ER -
KADEŘÁVEK, Pavel, F. FERRAGE, G. BODENHAUSEN and D. KURZBACH. High-Resolution NMR of Folded Proteins in Hyperpolarized Physiological Solvents. \textit{Chemistry - A European Journal}. WEINHEIM: Wiley, 2018, vol.~24, No~51, p.~13418-13423. ISSN~0947-6539. Available from: https://dx.doi.org/10.1002/chem.201802885.
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