Differences in Interleukin-8 Plasma Levels between Diabetic
Patients and Healthy Individuals Independently on Their
Periodontal Status

J 2018

Differences in Interleukin-8 Plasma Levels between Diabetic Patients and Healthy Individuals Independently on Their Periodontal Status

BOŘILOVÁ LINHARTOVÁ, Petra; Denisa KAVŘÍKOVÁ; Marie TOMANDLOVÁ; Hana POSKEROVÁ; Václav ŘEHKA et. al.

Základní údaje

Originální název

Differences in Interleukin-8 Plasma Levels between Diabetic Patients and Healthy Individuals Independently on Their Periodontal Status

Autoři

BOŘILOVÁ LINHARTOVÁ, Petra (203 Česká republika, domácí); Denisa KAVŘÍKOVÁ (203 Česká republika, domácí); Marie TOMANDLOVÁ (203 Česká republika, domácí); Hana POSKEROVÁ (203 Česká republika, domácí); Václav ŘEHKA (203 Česká republika, domácí); Ladislav DUŠEK (203 Česká republika, domácí) a Lydie IZAKOVIČOVÁ HOLLÁ (203 Česká republika, garant, domácí)

Vydání

International Journal of Molecular Sciences, Basel, Switzerland, MDPI AG, 2018, 1422-0067

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10608 Biochemistry and molecular biology

Stát vydavatele

Švýcarsko

Utajení

není předmětem státního či obchodního tajemství

Kód RIV

RIV/00216224:14110/18:00101349

Organizační jednotka

Lékařská fakulta

DOI

https://doi.org/10.3390/ijms19103214

UT WoS

000448951000369

EID Scopus

2-s2.0-85055080006

Klíčová slova anglicky

interleukin-8; chemokines; plasma; polymorphism; chronic periodontitis; diabetes mellitus

Štítky

14110130, 14110512, 14110518, 14119612, rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 9. 2. 2019 22:11, Soňa Böhmová

Anotace

V originále

Chronic periodontitis (CP) and diabetes mellitus (DM) involve several aspects of immune functions, including neutrophil activity and cytokine biology. Considering the critical function of chemokine interleukin-8 (IL-8) in the inflammatory process, the aims of this study were to determine: (i) IL-8 plasma levels; (ii) IL-8 (-251A/T, rs4073) and its receptor 2 (CXCR2, +1208C/T, rs1126579) polymorphisms, and (iii) the presence of the selected periodontal bacteria in types 1 and 2 DM patients (T1DM and T2DM) and systemically healthy controls (HC) with known periodontal status. This case-control study comprises of 153 unrelated individuals: 36/44 patients suffering from T1DM+CP/T2DM+CP and 32/41 from HC+CP/non-periodontitis HC. Both the clinical and biochemical parameters were monitored. The genotypes were determined using qPCR, IL-8 plasma levels were measured using an ELISA kit. Subgingival bacterial colonization was analyzed with a DNA microarray detection kit. The IL-8 plasma levels differed significantly between non-periodontitis HC and T1DM+CP/T2DM+CP patients (P < 0.01). Even in HC+CP, IL-8 concentrations were significantly lower than in T1DM+CP/T2DM+CP patients (P 0.05). No significant associations between the IL-8 plasma levels and the studied IL-8 and CXCR2 polymorphisms or the occurrence of selected periodontal bacteria (P > 0.05) were found. CP does not influence the circulating IL-8 levels. Patients with T1DM+CP/T2DM+CP had higher circulating IL-8 levels than HC+CP/non-periodontitis HC.

Návaznosti

GB14-37368G, projekt VaV

Název: Centrum orofaciálního vývoje a regenerace

Investor: Grantová agentura ČR, Centrum orofaciálního vývoje a regenerace

MUNI/A/1008/2017, interní kód MU

Název: Etiopatogenetické aspekty, diagnostika a léčba vybraných onemocnění dutiny ústní

Investor: Masarykova univerzita, Etiopatogenetické aspekty, diagnostika a léčba vybraných onemocnění dutiny ústní, DO R. 2020_Kategorie A - Specifický výzkum - Studentské výzkumné projekty

ROZV/25/LF/2017, interní kód MU

Název: LF - Příspěvek na IP 2017

Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, LF - Příspěvek na IP 2017, Interní rozvojové projekty

Citovat

BOŘILOVÁ LINHARTOVÁ, Petra; Denisa KAVŘÍKOVÁ; Marie TOMANDLOVÁ; Hana POSKEROVÁ; Václav ŘEHKA; Ladislav DUŠEK a Lydie IZAKOVIČOVÁ HOLLÁ. Differences in Interleukin-8 Plasma Levels between Diabetic Patients and Healthy Individuals Independently on Their Periodontal Status. International Journal of Molecular Sciences. Basel, Switzerland: MDPI AG, 2018, roč. 19, č. 10, s. 1-17. ISSN 1422-0067. Dostupné z: https://doi.org/10.3390/ijms19103214.

@article{1468996,
   author = {Bořilová Linhartová, Petra and Kavříková, Denisa and Tomandlová, Marie and Poskerová, Hana and Řehka, Václav and Dušek, Ladislav and Izakovičová Hollá, Lydie},
   article_location = {Basel, Switzerland},
   article_number = {10},
   doi = {https://doi.org/10.3390/ijms19103214},
   keywords = {interleukin-8; chemokines; plasma; polymorphism; chronic periodontitis; diabetes mellitus},
   language = {eng},
   issn = {1422-0067},
   journal = {International Journal of Molecular Sciences},
   title = {Differences in Interleukin-8 Plasma Levels between Diabetic Patients and Healthy Individuals Independently on Their Periodontal Status},
   volume = {19},
   year = {2018}
}

TY  - JOUR
ID  - 1468996
AU  - Bořilová Linhartová, Petra - Kavříková, Denisa - Tomandlová, Marie - Poskerová, Hana - Řehka, Václav - Dušek, Ladislav - Izakovičová Hollá, Lydie
PY  - 2018
TI  - Differences in Interleukin-8 Plasma Levels between Diabetic Patients and Healthy Individuals Independently on Their Periodontal Status
JF  - International Journal of Molecular Sciences
VL  - 19
IS  - 10
SP  - 1-17
EP  - 1-17
PB  - MDPI AG
SN  - 14220067
KW  - interleukin-8
KW  - chemokines
KW  - plasma
KW  - polymorphism
KW  - chronic periodontitis
KW  - diabetes mellitus
N2  - Chronic periodontitis (CP) and diabetes mellitus (DM) involve several aspects of immune functions, including neutrophil activity and cytokine biology. Considering the critical function of chemokine interleukin-8 (IL-8) in the inflammatory process, the aims of this study were to determine: (i) IL-8 plasma levels; (ii) IL-8 (-251A/T, rs4073) and its receptor 2 (CXCR2, +1208C/T, rs1126579) polymorphisms, and (iii) the presence of the selected periodontal bacteria in types 1 and 2 DM patients (T1DM and T2DM) and systemically healthy controls (HC) with known periodontal status. This case-control study comprises of 153 unrelated individuals: 36/44 patients suffering from T1DM+CP/T2DM+CP and 32/41 from HC+CP/non-periodontitis HC. Both the clinical and biochemical parameters were monitored. The genotypes were determined using qPCR, IL-8 plasma levels were measured using an ELISA kit. Subgingival bacterial colonization was analyzed with a DNA microarray detection kit. The IL-8 plasma levels differed significantly between non-periodontitis HC and T1DM+CP/T2DM+CP patients (P < 0.01). Even in HC+CP, IL-8 concentrations were significantly lower than in T1DM+CP/T2DM+CP patients (P 0.05). No significant associations between the IL-8 plasma levels and the studied IL-8 and CXCR2 polymorphisms or the occurrence of selected periodontal bacteria (P > 0.05) were found. CP does not influence the circulating IL-8 levels. Patients with T1DM+CP/T2DM+CP had higher circulating IL-8 levels than HC+CP/non-periodontitis HC.
ER  -

BOŘILOVÁ LINHARTOVÁ, Petra; Denisa KAVŘÍKOVÁ; Marie TOMANDLOVÁ; Hana POSKEROVÁ; Václav ŘEHKA; Ladislav DUŠEK a Lydie IZAKOVIČOVÁ HOLLÁ. Differences in Interleukin-8 Plasma Levels between Diabetic Patients and Healthy Individuals Independently on Their Periodontal Status. \textit{International Journal of Molecular Sciences}. Basel, Switzerland: MDPI AG, 2018, roč.~19, č.~10, s.~1-17. ISSN~1422-0067. Dostupné z: https://doi.org/10.3390/ijms19103214.

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