Differences in Interleukin-8 Plasma Levels between Diabetic
Patients and Healthy Individuals Independently on Their
Periodontal Status

J 2018

Differences in Interleukin-8 Plasma Levels between Diabetic Patients and Healthy Individuals Independently on Their Periodontal Status

BOŘILOVÁ LINHARTOVÁ, Petra, Denisa KAVŘÍKOVÁ, Marie TOMANDLOVÁ, Hana POSKEROVÁ, Václav ŘEHKA et. al.

Basic information

Original name

Differences in Interleukin-8 Plasma Levels between Diabetic Patients and Healthy Individuals Independently on Their Periodontal Status

Authors

BOŘILOVÁ LINHARTOVÁ, Petra (203 Czech Republic, belonging to the institution), Denisa KAVŘÍKOVÁ (203 Czech Republic, belonging to the institution), Marie TOMANDLOVÁ (203 Czech Republic, belonging to the institution), Hana POSKEROVÁ (203 Czech Republic, belonging to the institution), Václav ŘEHKA (203 Czech Republic, belonging to the institution), Ladislav DUŠEK (203 Czech Republic, belonging to the institution) and Lydie IZAKOVIČOVÁ HOLLÁ (203 Czech Republic, guarantor, belonging to the institution)

Edition

International Journal of Molecular Sciences, Basel, Switzerland, MDPI AG, 2018, 1422-0067

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10608 Biochemistry and molecular biology

Country of publisher

Switzerland

Confidentiality degree

není předmětem státního či obchodního tajemství

Impact factor

Impact factor: 4.183

RIV identification code

RIV/00216224:14110/18:00101349

Organization unit

Faculty of Medicine

DOI

http://dx.doi.org/10.3390/ijms19103214

UT WoS

000448951000369

Keywords in English

interleukin-8; chemokines; plasma; polymorphism; chronic periodontitis; diabetes mellitus

Abstract

V originále

Chronic periodontitis (CP) and diabetes mellitus (DM) involve several aspects of immune functions, including neutrophil activity and cytokine biology. Considering the critical function of chemokine interleukin-8 (IL-8) in the inflammatory process, the aims of this study were to determine: (i) IL-8 plasma levels; (ii) IL-8 (-251A/T, rs4073) and its receptor 2 (CXCR2, +1208C/T, rs1126579) polymorphisms, and (iii) the presence of the selected periodontal bacteria in types 1 and 2 DM patients (T1DM and T2DM) and systemically healthy controls (HC) with known periodontal status. This case-control study comprises of 153 unrelated individuals: 36/44 patients suffering from T1DM+CP/T2DM+CP and 32/41 from HC+CP/non-periodontitis HC. Both the clinical and biochemical parameters were monitored. The genotypes were determined using qPCR, IL-8 plasma levels were measured using an ELISA kit. Subgingival bacterial colonization was analyzed with a DNA microarray detection kit. The IL-8 plasma levels differed significantly between non-periodontitis HC and T1DM+CP/T2DM+CP patients (P < 0.01). Even in HC+CP, IL-8 concentrations were significantly lower than in T1DM+CP/T2DM+CP patients (P 0.05). No significant associations between the IL-8 plasma levels and the studied IL-8 and CXCR2 polymorphisms or the occurrence of selected periodontal bacteria (P > 0.05) were found. CP does not influence the circulating IL-8 levels. Patients with T1DM+CP/T2DM+CP had higher circulating IL-8 levels than HC+CP/non-periodontitis HC.

Links

GB14-37368G, research and development project

Name: Centrum orofaciálního vývoje a regenerace

Investor: Czech Science Foundation

MUNI/A/1008/2017, interní kód MU

Name: Etiopatogenetické aspekty, diagnostika a léčba vybraných onemocnění dutiny ústní

Investor: Masaryk University, Category A

ROZV/25/LF/2017, interní kód MU

Name: LF - Příspěvek na IP 2017

Investor: Ministry of Education, Youth and Sports of the CR, Internal development projects

Citovat

BOŘILOVÁ LINHARTOVÁ, Petra, Denisa KAVŘÍKOVÁ, Marie TOMANDLOVÁ, Hana POSKEROVÁ, Václav ŘEHKA, Ladislav DUŠEK and Lydie IZAKOVIČOVÁ HOLLÁ. Differences in Interleukin-8 Plasma Levels between Diabetic Patients and Healthy Individuals Independently on Their Periodontal Status. International Journal of Molecular Sciences. Basel, Switzerland: MDPI AG, 2018, vol. 19, No 10, p. 1-17. ISSN 1422-0067. Available from: https://dx.doi.org/10.3390/ijms19103214.

@article{1468996,
   author = {Bořilová Linhartová, Petra and Kavříková, Denisa and Tomandlová, Marie and Poskerová, Hana and Řehka, Václav and Dušek, Ladislav and Izakovičová Hollá, Lydie},
   article_location = {Basel, Switzerland},
   article_number = {10},
   doi = {http://dx.doi.org/10.3390/ijms19103214},
   keywords = {interleukin-8; chemokines; plasma; polymorphism; chronic periodontitis; diabetes mellitus},
   language = {eng},
   issn = {1422-0067},
   journal = {International Journal of Molecular Sciences},
   title = {Differences in Interleukin-8 Plasma Levels between Diabetic Patients and Healthy Individuals Independently on Their Periodontal Status},
   volume = {19},
   year = {2018}
}

TY  - JOUR
ID  - 1468996
AU  - Bořilová Linhartová, Petra - Kavříková, Denisa - Tomandlová, Marie - Poskerová, Hana - Řehka, Václav - Dušek, Ladislav - Izakovičová Hollá, Lydie
PY  - 2018
TI  - Differences in Interleukin-8 Plasma Levels between Diabetic Patients and Healthy Individuals Independently on Their Periodontal Status
JF  - International Journal of Molecular Sciences
VL  - 19
IS  - 10
SP  - 1-17
EP  - 1-17
PB  - MDPI AG
SN  - 14220067
KW  - interleukin-8
KW  - chemokines
KW  - plasma
KW  - polymorphism
KW  - chronic periodontitis
KW  - diabetes mellitus
N2  - Chronic periodontitis (CP) and diabetes mellitus (DM) involve several aspects of immune functions, including neutrophil activity and cytokine biology. Considering the critical function of chemokine interleukin-8 (IL-8) in the inflammatory process, the aims of this study were to determine: (i) IL-8 plasma levels; (ii) IL-8 (-251A/T, rs4073) and its receptor 2 (CXCR2, +1208C/T, rs1126579) polymorphisms, and (iii) the presence of the selected periodontal bacteria in types 1 and 2 DM patients (T1DM and T2DM) and systemically healthy controls (HC) with known periodontal status. This case-control study comprises of 153 unrelated individuals: 36/44 patients suffering from T1DM+CP/T2DM+CP and 32/41 from HC+CP/non-periodontitis HC. Both the clinical and biochemical parameters were monitored. The genotypes were determined using qPCR, IL-8 plasma levels were measured using an ELISA kit. Subgingival bacterial colonization was analyzed with a DNA microarray detection kit. The IL-8 plasma levels differed significantly between non-periodontitis HC and T1DM+CP/T2DM+CP patients (P < 0.01). Even in HC+CP, IL-8 concentrations were significantly lower than in T1DM+CP/T2DM+CP patients (P 0.05). No significant associations between the IL-8 plasma levels and the studied IL-8 and CXCR2 polymorphisms or the occurrence of selected periodontal bacteria (P > 0.05) were found. CP does not influence the circulating IL-8 levels. Patients with T1DM+CP/T2DM+CP had higher circulating IL-8 levels than HC+CP/non-periodontitis HC.
ER  -

BOŘILOVÁ LINHARTOVÁ, Petra, Denisa KAVŘÍKOVÁ, Marie TOMANDLOVÁ, Hana POSKEROVÁ, Václav ŘEHKA, Ladislav DUŠEK and Lydie IZAKOVIČOVÁ HOLLÁ. Differences in Interleukin-8 Plasma Levels between Diabetic Patients and Healthy Individuals Independently on Their Periodontal Status. \textit{International Journal of Molecular Sciences}. Basel, Switzerland: MDPI AG, 2018, vol.~19, No~10, p.~1-17. ISSN~1422-0067. Available from: https://dx.doi.org/10.3390/ijms19103214.

Detailed Information on Publication Record