KAHOUNOVÁ, Zuzana, Eva SLABÁKOVÁ, Lucia BINÓ, Ján REMŠÍK, Radek FEDR, Jan BOUCHAL, Daniela KURFŰRSTOVÁ, Radek VRTĚL, Vladimír ŠTUDENT, Lucie JUREČKOVÁ, Volodymyr POROKH, Aleš HAMPL and Karel SOUČEK. Generation of human iPSCs from human prostate cancer-associated fibroblasts IBPi002-A. Stem Cell Research. Amsterdam: ELSEVIER SCIENCE, 2018, vol. 33, DEC 2018, p. 255-259. ISSN 1873-5061. Available from: https://dx.doi.org/10.1016/j.scr.2018.11.006.
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Basic information
Original name Generation of human iPSCs from human prostate cancer-associated fibroblasts IBPi002-A
Authors KAHOUNOVÁ, Zuzana (203 Czech Republic), Eva SLABÁKOVÁ (203 Czech Republic), Lucia BINÓ (703 Slovakia), Ján REMŠÍK (703 Slovakia), Radek FEDR (203 Czech Republic), Jan BOUCHAL (203 Czech Republic), Daniela KURFŰRSTOVÁ (203 Czech Republic), Radek VRTĚL (203 Czech Republic), Vladimír ŠTUDENT (203 Czech Republic), Lucie JUREČKOVÁ (203 Czech Republic), Volodymyr POROKH (804 Ukraine, belonging to the institution), Aleš HAMPL (203 Czech Republic, belonging to the institution) and Karel SOUČEK (203 Czech Republic, guarantor).
Edition Stem Cell Research, Amsterdam, ELSEVIER SCIENCE, 2018, 1873-5061.
Other information
Original language English
Type of outcome Article in a journal
Field of Study 10601 Cell biology
Country of publisher Netherlands
Confidentiality degree is not subject to a state or trade secret
WWW URL
Impact factor Impact factor: 3.929
RIV identification code RIV/00216224:14110/18:00101364
Organization unit Faculty of Medicine
Doi http://dx.doi.org/10.1016/j.scr.2018.11.006
UT WoS 000452639700039
Keywords in English prostate cancer
Tags 14110517, rivok
Tags International impact, Reviewed
Changed by Changed by: Mgr. Tereza Miškechová, učo 341652. Changed: 26/5/2021 08:35.
Abstract
A human induced pluripotent stem cell line was generated from cancer-associated fibroblasts of a 68-years old patient with diagnosed prostate adenocarcinoma (PCa). The fibroblast cell line was reprogrammed with Epi5 (TM) Episomal iPSC Reprogramming Kit. Pluripotency of the derived transgene-free iPS cell line was confirmed both in vitro by detecting expression of factors of pluripotency on a single-cell level, and also in vivo using teratoma formation assay. This new iPS cell line may be used for differentiation into different prostate-specific cell types in differentiation studies.
Links
GA15-11707S, research and development projectName: Centrosomální abnormality u lidských pluripotentních kmenových buněk.
Investor: Czech Science Foundation
LM2015062, research and development projectName: Národní infrastruktura pro biologické a medicínské zobrazování
Investor: Ministry of Education, Youth and Sports of the CR
MUNI/C/1418/2017, interní kód MUName: Extracelulární faktory ovlivňující metabolismus centrozomů v lidských pluripotentních kmenových buňkách
Investor: Masaryk University, Rector's Program
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