The Most Basin represents a coal basin with a long mining history in the Czech Republic. The majority of palaeo-environmental and geochemical studies have been focused on the upper part of the coal-bearing Most Formation where the coal is generally enriched in As, Cr, Ni, Cu and Zn. This study evaluated the distribution of V, Cr, Ni, Cu, Zn, Pb, Se, As, and Mn from the lower part. Regarding to the transport of inorganic elements into environment, the evaluation of mobility potential was also conducted. The total of 51 samples were collected from the Lower Bench in the Holesice Member. The chemical analyses were performed together with optical microscopy. The X-ray spectroscopy and ICP-MS were used to detect trace element contents in the study set of samples. 4 samples were subjected to the Sequential Extraction Procedure. The prepared leachates were analysed using the ICP-MS method. In total, 13 elements were screened in samples within the study profile. The mode of occurrence is the key factor for identifying a potential source of the trace elements. All evaluated trace elements (V, Cr, Ni, Cu, Zn, Pb, Se, As and Mn) leached most in the oxidisable fraction. On the other hand, As, Mn, Ni, Zn and Cd leached in more mobile fractions, e.g. reducible and acid.