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@article{1471036, author = {Buchtová, Žaneta and Lacková, Zuzana and Kudr, Jiří and Škoda, Jan and Zítka, Ondřej and Zítka, Zdeněk}, article_number = {říjen 2018}, doi = {http://dx.doi.org/10.3390/molecules23102504}, keywords = {antioxidant molecules; blood drop analysis; electrochemical analysis; nutritional study; sample pretreatment}, language = {eng}, issn = {1420-3049}, journal = {Molecules}, title = {Capillary Blood GSH Level Monitoring, Using an Electrochemical Method Adapted for Micro Volumes}, url = {https://www.mdpi.com/1420-3049/23/10/2504}, volume = {23}, year = {2018} }
TY - JOUR ID - 1471036 AU - Buchtová, Žaneta - Lacková, Zuzana - Kudr, Jiří - Škoda, Jan - Zítka, Ondřej - Zítka, Zdeněk PY - 2018 TI - Capillary Blood GSH Level Monitoring, Using an Electrochemical Method Adapted for Micro Volumes JF - Molecules VL - 23 IS - říjen 2018 SP - 1-10 EP - 1-10 PB - Mayer und Muller SN - 14203049 KW - antioxidant molecules KW - blood drop analysis KW - electrochemical analysis KW - nutritional study KW - sample pretreatment UR - https://www.mdpi.com/1420-3049/23/10/2504 L2 - https://www.mdpi.com/1420-3049/23/10/2504 N2 - Glutathione ( -glutamyl-cysteinyl-glycine; also known as GSH) is an endogenous antioxidant that plays a crucial role in cell defense mechanisms against oxidative stress. It is thus not surprising that this molecule can serve as a biomarker for oxidative stress monitoring. As capillary blood is a highly accessible target for biomarking, it is a valuable bodily fluid for diagnosing human GSH levels. This study focused on the optimization of GSH measurements from micro volumes of capillary blood prior to using electrochemical detection. The optimization of experimental parameters, including the sample volume and its stability, was performed and evaluated. Moreover, we tested the optimized method as part of a short-term study. The study consisted of examining 10 subjects within 96 h of their consumption of high amounts of antioxidants, attained from a daily dose of 2 g/150 mL of green tea. The subjects’ capillary blood (5 L) was taken at 0 h, 48 h, and 96 h for subsequent analysis. The short-term supplementation of diet with green tea ER -
BUCHTOVÁ, Žaneta, Zuzana LACKOVÁ, Jiří KUDR, Jan ŠKODA, Ondřej ZÍTKA and Zdeněk ZÍTKA. Capillary Blood GSH Level Monitoring, Using an Electrochemical Method Adapted for Micro Volumes. \textit{Molecules}. Mayer und Muller, 2018, vol.~23, říjen 2018, p.~1-10. ISSN~1420-3049. Available from: https://dx.doi.org/10.3390/molecules23102504.
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