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@article{1473185, author = {Ożegowska, Katarzyna and DyszkiewiczandKonwinska, Marta and Celichowski, Piotr and Nawrocki, Mariusz J. and Bryja, Artur and Jankowski, Maurycy and Kranc, Wiesława and Brazert, Maciej and Knap, Sandra and Ješeta, Michal and Skowronski, Mariusz T. and Bukowska, Dorota and Antosik, Paweł and Brussow, Klaus P. and Breborowicz, Andrzej and Bruska, Małgorzata and Nowicki, Michał and Pawelczyk, Lezsek and Zabel, Maciej and Kempisty, Bartosz}, article_location = {NEW YORK}, article_number = {NOV 2018}, doi = {http://dx.doi.org/10.1016/j.theriogenology.2018.08.019}, keywords = {Porcine oocyte; In vitro maturation; Sex differentiation; Microarrays}, language = {eng}, issn = {0093-691X}, journal = {THERIOGENOLOGY}, title = {Expression pattern of new genes regulating female sex differentiation and in vitro maturational status of oocytes in pigs}, volume = {121}, year = {2018} }
TY - JOUR ID - 1473185 AU - Ożegowska, Katarzyna - Dyszkiewicz-Konwinska, Marta - Celichowski, Piotr - Nawrocki, Mariusz J. - Bryja, Artur - Jankowski, Maurycy - Kranc, Wiesława - Brazert, Maciej - Knap, Sandra - Ješeta, Michal - Skowronski, Mariusz T. - Bukowska, Dorota - Antosik, Paweł - Brussow, Klaus P. - Breborowicz, Andrzej - Bruska, Małgorzata - Nowicki, Michał - Pawelczyk, Lezsek - Zabel, Maciej - Kempisty, Bartosz PY - 2018 TI - Expression pattern of new genes regulating female sex differentiation and in vitro maturational status of oocytes in pigs JF - THERIOGENOLOGY VL - 121 IS - NOV 2018 SP - 122-133 EP - 122-133 PB - ELSEVIER SCIENCE INC SN - 0093691X KW - Porcine oocyte KW - In vitro maturation KW - Sex differentiation KW - Microarrays N2 - The processes underlying maturation of mammalian oocytes are considered crucial for the oocytes ability to undergo monospermic fertilization. The same factors of influence are suggested to impact the development of sex associated characteristics, allowing sex differentiation to progress during embryonic growth. The primary aim of the study was to analyze the gene ontology groups involved in regulation of porcine oocytes' response to endogenous stimuli. The results obtained would indicate potential genes influencing sex differentiation. Additionally, they could help to determine new genetic markers, expression profile of which is substantially regulated during porcine oocytes' in vitro maturation. To achieve that, porcine oocytes were collected for analysis before and after in vitro maturation. Pigs were used as they are a readily available model that presents significant similarity to humans in terms of physiology and anatomy. Microarray analysis of oocytes, before and after in vitro maturation was performed and later validated by RT-qPCR. We have particularly detected and analyzed genes belonging to gene ontology groups associated with hormonal stimulation during maturation of the oocytes, that exhibited significant change in expression (fold change >=vertical bar 2 vertical bar; p < 0.05) namely "Female sex differentiation" (CCND2, MMP14, VEGFA, FST, INHBA, NR5A1), "Response to endogenous stimulus" (INSR, ESR1, CCND2, TXNIP, TACR3, MMP14, FOS, AR, EGR2, IGEBP7, TGFBR3, BTG2, PLD1, PHIP, UBE2B) and "Response to estrogen stimulus" (INSR, ESR1, CCND2, IHH, TXNIP, TACR3, MMP14). Some of them were characteristic for just one of the described ontologies, while some belonged into multiple ontological terms. The genes were analyzed, with their relation to the processes of interest explained. Overall, the study provides us with a range of genes that might serve as molecular markers of in vitro maturation associated processes of the oocytes. This knowledge might serve as a reference for further studies and, after further validation, as a potentially useful knowledge in assessment of the oocytes during assisted reproduction processes. (C) 2018 Elsevier Inc. All rights reserved. ER -
O$\backslash$.ZEGOWSKA, Katarzyna, Marta DYSZKIEWICZ-KONWINSKA, Piotr CELICHOWSKI, Mariusz J. NAWROCKI, Artur BRYJA, Maurycy JANKOWSKI, Wiesława KRANC, Maciej BRAZERT, Sandra KNAP, Michal JEŠETA, Mariusz T. SKOWRONSKI, Dorota BUKOWSKA, Paweł ANTOSIK, Klaus P. BRUSSOW, Andrzej BREBOROWICZ, Małgorzata BRUSKA, Michał NOWICKI, Lezsek PAWELCZYK, Maciej ZABEL and Bartosz KEMPISTY. Expression pattern of new genes regulating female sex differentiation and in vitro maturational status of oocytes in pigs. \textit{THERIOGENOLOGY}. NEW YORK: ELSEVIER SCIENCE INC, 2018, vol.~121, NOV 2018, p.~122-133. ISSN~0093-691X. Available from: https://dx.doi.org/10.1016/j.theriogenology.2018.08.019.
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