Expression pattern of new genes regulating female sex
differentiation and in vitro maturational status of oocytes in
pigs

J 2018

Expression pattern of new genes regulating female sex differentiation and in vitro maturational status of oocytes in pigs

OŻEGOWSKA, Katarzyna, Marta DYSZKIEWICZ-KONWINSKA, Piotr CELICHOWSKI, Mariusz J. NAWROCKI, Artur BRYJA et. al.

Basic information

Original name

Expression pattern of new genes regulating female sex differentiation and in vitro maturational status of oocytes in pigs

Authors

OŻEGOWSKA, Katarzyna (616 Poland), Marta DYSZKIEWICZ-KONWINSKA (616 Poland), Piotr CELICHOWSKI (616 Poland), Mariusz J. NAWROCKI (616 Poland), Artur BRYJA (616 Poland), Maurycy JANKOWSKI (616 Poland), Wiesława KRANC (616 Poland), Maciej BRAZERT (616 Poland), Sandra KNAP (616 Poland), Michal JEŠETA (203 Czech Republic, belonging to the institution), Mariusz T. SKOWRONSKI (616 Poland), Dorota BUKOWSKA (616 Poland), Paweł ANTOSIK (616 Poland), Klaus P. BRUSSOW (616 Poland), Andrzej BREBOROWICZ (616 Poland), Małgorzata BRUSKA (616 Poland), Michał NOWICKI (616 Poland), Lezsek PAWELCZYK (616 Poland), Maciej ZABEL (616 Poland) and Bartosz KEMPISTY (616 Poland, guarantor)

Edition

THERIOGENOLOGY, NEW YORK, ELSEVIER SCIENCE INC, 2018, 0093-691X

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10604 Reproductive biology

Country of publisher

United States of America

Confidentiality degree

není předmětem státního či obchodního tajemství

Impact factor

Impact factor: 2.299

RIV identification code

RIV/00216224:14110/18:00104698

Organization unit

Faculty of Medicine

DOI

http://dx.doi.org/10.1016/j.theriogenology.2018.08.019

UT WoS

000447478600018

Keywords in English

Porcine oocyte; In vitro maturation; Sex differentiation; Microarrays

Abstract

V originále

The processes underlying maturation of mammalian oocytes are considered crucial for the oocytes ability to undergo monospermic fertilization. The same factors of influence are suggested to impact the development of sex associated characteristics, allowing sex differentiation to progress during embryonic growth. The primary aim of the study was to analyze the gene ontology groups involved in regulation of porcine oocytes' response to endogenous stimuli. The results obtained would indicate potential genes influencing sex differentiation. Additionally, they could help to determine new genetic markers, expression profile of which is substantially regulated during porcine oocytes' in vitro maturation. To achieve that, porcine oocytes were collected for analysis before and after in vitro maturation. Pigs were used as they are a readily available model that presents significant similarity to humans in terms of physiology and anatomy. Microarray analysis of oocytes, before and after in vitro maturation was performed and later validated by RT-qPCR. We have particularly detected and analyzed genes belonging to gene ontology groups associated with hormonal stimulation during maturation of the oocytes, that exhibited significant change in expression (fold change >=vertical bar 2 vertical bar; p < 0.05) namely "Female sex differentiation" (CCND2, MMP14, VEGFA, FST, INHBA, NR5A1), "Response to endogenous stimulus" (INSR, ESR1, CCND2, TXNIP, TACR3, MMP14, FOS, AR, EGR2, IGEBP7, TGFBR3, BTG2, PLD1, PHIP, UBE2B) and "Response to estrogen stimulus" (INSR, ESR1, CCND2, IHH, TXNIP, TACR3, MMP14). Some of them were characteristic for just one of the described ontologies, while some belonged into multiple ontological terms. The genes were analyzed, with their relation to the processes of interest explained. Overall, the study provides us with a range of genes that might serve as molecular markers of in vitro maturation associated processes of the oocytes. This knowledge might serve as a reference for further studies and, after further validation, as a potentially useful knowledge in assessment of the oocytes during assisted reproduction processes. (C) 2018 Elsevier Inc. All rights reserved.

Citovat

OŻEGOWSKA, Katarzyna, Marta DYSZKIEWICZ-KONWINSKA, Piotr CELICHOWSKI, Mariusz J. NAWROCKI, Artur BRYJA, Maurycy JANKOWSKI, Wiesława KRANC, Maciej BRAZERT, Sandra KNAP, Michal JEŠETA, Mariusz T. SKOWRONSKI, Dorota BUKOWSKA, Paweł ANTOSIK, Klaus P. BRUSSOW, Andrzej BREBOROWICZ, Małgorzata BRUSKA, Michał NOWICKI, Lezsek PAWELCZYK, Maciej ZABEL and Bartosz KEMPISTY. Expression pattern of new genes regulating female sex differentiation and in vitro maturational status of oocytes in pigs. THERIOGENOLOGY. NEW YORK: ELSEVIER SCIENCE INC, 2018, vol. 121, NOV 2018, p. 122-133. ISSN 0093-691X. Available from: https://dx.doi.org/10.1016/j.theriogenology.2018.08.019.

@article{1473185,
   author = {Ożegowska, Katarzyna and DyszkiewiczandKonwinska, Marta and Celichowski, Piotr and Nawrocki, Mariusz J. and Bryja, Artur and Jankowski, Maurycy and Kranc, Wiesława and Brazert, Maciej and Knap, Sandra and Ješeta, Michal and Skowronski, Mariusz T. and Bukowska, Dorota and Antosik, Paweł and Brussow, Klaus P. and Breborowicz, Andrzej and Bruska, Małgorzata and Nowicki, Michał and Pawelczyk, Lezsek and Zabel, Maciej and Kempisty, Bartosz},
   article_location = {NEW YORK},
   article_number = {NOV 2018},
   doi = {http://dx.doi.org/10.1016/j.theriogenology.2018.08.019},
   keywords = {Porcine oocyte; In vitro maturation; Sex differentiation; Microarrays},
   language = {eng},
   issn = {0093-691X},
   journal = {THERIOGENOLOGY},
   title = {Expression pattern of new genes regulating female sex differentiation and in vitro maturational status of oocytes in pigs},
   volume = {121},
   year = {2018}
}

TY  - JOUR
ID  - 1473185
AU  - Ożegowska, Katarzyna - Dyszkiewicz-Konwinska, Marta - Celichowski, Piotr - Nawrocki, Mariusz J. - Bryja, Artur - Jankowski, Maurycy - Kranc, Wiesława - Brazert, Maciej - Knap, Sandra - Ješeta, Michal - Skowronski, Mariusz T. - Bukowska, Dorota - Antosik, Paweł - Brussow, Klaus P. - Breborowicz, Andrzej - Bruska, Małgorzata - Nowicki, Michał - Pawelczyk, Lezsek - Zabel, Maciej - Kempisty, Bartosz
PY  - 2018
TI  - Expression pattern of new genes regulating female sex differentiation and in vitro maturational status of oocytes in pigs
JF  - THERIOGENOLOGY
VL  - 121
IS  - NOV 2018
SP  - 122-133
EP  - 122-133
PB  - ELSEVIER SCIENCE INC
SN  - 0093691X
KW  - Porcine oocyte
KW  - In vitro maturation
KW  - Sex differentiation
KW  - Microarrays
N2  - The processes underlying maturation of mammalian oocytes are considered crucial for the oocytes ability to undergo monospermic fertilization. The same factors of influence are suggested to impact the development of sex associated characteristics, allowing sex differentiation to progress during embryonic growth. The primary aim of the study was to analyze the gene ontology groups involved in regulation of porcine oocytes' response to endogenous stimuli. The results obtained would indicate potential genes influencing sex differentiation. Additionally, they could help to determine new genetic markers, expression profile of which is substantially regulated during porcine oocytes' in vitro maturation. To achieve that, porcine oocytes were collected for analysis before and after in vitro maturation. Pigs were used as they are a readily available model that presents significant similarity to humans in terms of physiology and anatomy. Microarray analysis of oocytes, before and after in vitro maturation was performed and later validated by RT-qPCR. We have particularly detected and analyzed genes belonging to gene ontology groups associated with hormonal stimulation during maturation of the oocytes, that exhibited significant change in expression (fold change >=vertical bar 2 vertical bar; p < 0.05) namely "Female sex differentiation" (CCND2, MMP14, VEGFA, FST, INHBA, NR5A1), "Response to endogenous stimulus" (INSR, ESR1, CCND2, TXNIP, TACR3, MMP14, FOS, AR, EGR2, IGEBP7, TGFBR3, BTG2, PLD1, PHIP, UBE2B) and "Response to estrogen stimulus" (INSR, ESR1, CCND2, IHH, TXNIP, TACR3, MMP14). Some of them were characteristic for just one of the described ontologies, while some belonged into multiple ontological terms. The genes were analyzed, with their relation to the processes of interest explained. Overall, the study provides us with a range of genes that might serve as molecular markers of in vitro maturation associated processes of the oocytes. This knowledge might serve as a reference for further studies and, after further validation, as a potentially useful knowledge in assessment of the oocytes during assisted reproduction processes. (C) 2018 Elsevier Inc. All rights reserved.
ER  -

O$\backslash$.ZEGOWSKA, Katarzyna, Marta DYSZKIEWICZ-KONWINSKA, Piotr CELICHOWSKI, Mariusz J. NAWROCKI, Artur BRYJA, Maurycy JANKOWSKI, Wiesława KRANC, Maciej BRAZERT, Sandra KNAP, Michal JEŠETA, Mariusz T. SKOWRONSKI, Dorota BUKOWSKA, Paweł ANTOSIK, Klaus P. BRUSSOW, Andrzej BREBOROWICZ, Małgorzata BRUSKA, Michał NOWICKI, Lezsek PAWELCZYK, Maciej ZABEL and Bartosz KEMPISTY. Expression pattern of new genes regulating female sex differentiation and in vitro maturational status of oocytes in pigs. \textit{THERIOGENOLOGY}. NEW YORK: ELSEVIER SCIENCE INC, 2018, vol.~121, NOV 2018, p.~122-133. ISSN~0093-691X. Available from: https://dx.doi.org/10.1016/j.theriogenology.2018.08.019.

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