KORSÁK, Marek, Josef HOUSER, Eva FUJDIAROVÁ, Marie POKORNÁ, Jan KOMÁREK and Michaela WIMMEROVÁ. Characterization of PluLec, a novel galactose-specific lectin from Photorhabdus luminescens. In XIX. setkání biochemiků a molekulárních biologů. 2018. ISBN 978-80-210-9069-9.
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Basic information
Original name Characterization of PluLec, a novel galactose-specific lectin from Photorhabdus luminescens
Authors KORSÁK, Marek (703 Slovakia, belonging to the institution), Josef HOUSER (203 Czech Republic, belonging to the institution), Eva FUJDIAROVÁ (203 Czech Republic, belonging to the institution), Marie POKORNÁ (203 Czech Republic, belonging to the institution), Jan KOMÁREK (203 Czech Republic, belonging to the institution) and Michaela WIMMEROVÁ (203 Czech Republic, guarantor, belonging to the institution).
Edition XIX. setkání biochemiků a molekulárních biologů, 2018.
Other information
Original language English
Type of outcome Conference abstract
Field of Study 10600 1.6 Biological sciences
Country of publisher Czech Republic
Confidentiality degree is not subject to a state or trade secret
RIV identification code RIV/00216224:14310/18:00104710
Organization unit Faculty of Science
ISBN 978-80-210-9069-9
Keywords (in Czech) Photorhabdus luminescens;lektin;
Keywords in English Photorhabdus luminescens;lectin
Changed by Changed by: Mgr. Marek Korsák, učo 436513. Changed: 29/11/2018 16:49.
Abstract
Lectins are proteins of non-immune origin capable of binding carbohydrates reversibly and with high specificity. They are found in all known living organisms. Unlike antibodies, they are not product of immune response and do not possess any enzymatic activity. Lectins are involved in recognition events in various physiological and pathological processes (e.g. cell communication, adhesion, migration, pathogen recognition, immune response modulation). Lectins are commonly used for characterization of carbohydrate structures, for purification of glycoproteins and to specifically label the cell surface structures. They are also used for separation and cell typing. Photorhabdus luminescens is a naturally bioluminescent Gram-negative bacterium and an insect pathogen, which symbiotically lives in Heterorhabditidae nematodes. PluLec is a putative lectin from Photorhabdus luminescens and a homologue of PA-IL lectin, which is D-galactose specific, Ca2+ dependent, cytotoxic lectin from opportunistic pathogen Pseudomonas aeruginosa, involved in facilitating infection in patients with compromised immunity. This research is focused on cloning, production and study of recombinant protein PluLec using various methods for structural and functional characterization, namely dynamic light scattering, circular dichroism, differential scanning fluorimetry, hemagglutination, glycan array, surface plasmon resonance, analytical ultracentrifugation and protein X-ray crystallography. Detailed knowledge of the structure and function of PluLec can help to understand the mechanism of P. luminescens infection. The lectin might also find further application in biotechnological research.
Links
MUNI/A/1100/2017, interní kód MUName: Podpora biochemického výzkumu v roce 2018
Investor: Masaryk University, Category A
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