a 2018

Characterization of PluLec, a novel galactose-specific lectin from Photorhabdus luminescens

KORSÁK, Marek, Josef HOUSER, Eva FUJDIAROVÁ, Marie POKORNÁ, Jan KOMÁREK et. al.

Basic information

Original name

Characterization of PluLec, a novel galactose-specific lectin from Photorhabdus luminescens

Authors

KORSÁK, Marek (703 Slovakia, belonging to the institution), Josef HOUSER (203 Czech Republic, belonging to the institution), Eva FUJDIAROVÁ (203 Czech Republic, belonging to the institution), Marie POKORNÁ (203 Czech Republic, belonging to the institution), Jan KOMÁREK (203 Czech Republic, belonging to the institution) and Michaela WIMMEROVÁ (203 Czech Republic, guarantor, belonging to the institution)

Edition

XIX. setkání biochemiků a molekulárních biologů, 2018

Other information

Language

English

Type of outcome

Konferenční abstrakt

Field of Study

10600 1.6 Biological sciences

Country of publisher

Czech Republic

Confidentiality degree

není předmětem státního či obchodního tajemství

RIV identification code

RIV/00216224:14310/18:00104710

Organization unit

Faculty of Science

ISBN

978-80-210-9069-9

Keywords (in Czech)

Photorhabdus luminescens;lektin;

Keywords in English

Photorhabdus luminescens;lectin
Změněno: 29/11/2018 16:49, Mgr. Marek Korsák

Abstract

V originále

Lectins are proteins of non-immune origin capable of binding carbohydrates reversibly and with high specificity. They are found in all known living organisms. Unlike antibodies, they are not product of immune response and do not possess any enzymatic activity. Lectins are involved in recognition events in various physiological and pathological processes (e.g. cell communication, adhesion, migration, pathogen recognition, immune response modulation). Lectins are commonly used for characterization of carbohydrate structures, for purification of glycoproteins and to specifically label the cell surface structures. They are also used for separation and cell typing. Photorhabdus luminescens is a naturally bioluminescent Gram-negative bacterium and an insect pathogen, which symbiotically lives in Heterorhabditidae nematodes. PluLec is a putative lectin from Photorhabdus luminescens and a homologue of PA-IL lectin, which is D-galactose specific, Ca2+ dependent, cytotoxic lectin from opportunistic pathogen Pseudomonas aeruginosa, involved in facilitating infection in patients with compromised immunity. This research is focused on cloning, production and study of recombinant protein PluLec using various methods for structural and functional characterization, namely dynamic light scattering, circular dichroism, differential scanning fluorimetry, hemagglutination, glycan array, surface plasmon resonance, analytical ultracentrifugation and protein X-ray crystallography. Detailed knowledge of the structure and function of PluLec can help to understand the mechanism of P. luminescens infection. The lectin might also find further application in biotechnological research.

Links

MUNI/A/1100/2017, interní kód MU
Name: Podpora biochemického výzkumu v roce 2018
Investor: Masaryk University, Category A