Expression of Regeneration-Associated Proteins in Primary
Sensory Neurons and Regenerating Axons After Nerve Injury-An
Overview

J 2018

Expression of Regeneration-Associated Proteins in Primary Sensory Neurons and Regenerating Axons After Nerve Injury-An Overview

DUBOVÝ, Petr, Ilona KLUSÁKOVÁ, Ivana HRADILOVÁ SVÍŽENSKÁ and Marek JOUKAL

Basic information

Original name

Expression of Regeneration-Associated Proteins in Primary Sensory Neurons and Regenerating Axons After Nerve Injury-An Overview

Authors

DUBOVÝ, Petr (203 Czech Republic, guarantor, belonging to the institution), Ilona KLUSÁKOVÁ (203 Czech Republic, belonging to the institution), Ivana HRADILOVÁ SVÍŽENSKÁ (203 Czech Republic, belonging to the institution) and Marek JOUKAL (203 Czech Republic, belonging to the institution)

Edition

ANATOMICAL RECORD-ADVANCES IN INTEGRATIVE ANATOMY AND EVOLUTIONARY BIOLOGY, HOBOKEN, WILEY, 2018, 1932-8486

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

30106 Anatomy and morphology

Country of publisher

United States of America

Confidentiality degree

není předmětem státního či obchodního tajemství

Impact factor

Impact factor: 1.329

RIV identification code

RIV/00216224:14110/18:00101383

Organization unit

Faculty of Medicine

DOI

http://dx.doi.org/10.1002/ar.23843

UT WoS

000449639900004

Keywords in English

peripheral nerve; regeneration; degeneration; therapies

Abstract

V originále

Peripheral nerve injury results in profound alterations of the affected neurons resulting from the interplay between intrinsic and extrinsic molecular events. Restarting the neuronal regenerative program is an important prerequisite for functional recovery of the injured peripheral nerve. The primary sensory neurons with their cell bodies in the dorsal root ganglia provide a useful in vivo and in vitro model for studying the mechanisms that regulate intrinsic neuronal regeneration capacity following axotomy. These studies frequently need to indicate the regenerative status of the corresponding neurons. We summarize the critical issues regarding immunohistochemical detection of several regeneration-associated proteins as markers for the initiation of the regeneration program in rat primary sensory neurons and indicators of axon regeneration in the peripheral nerves. This overview also includes our own results of GAP43 and SCG10 expression in different DRG neurons following double immunostaining with molecular markers of neuronal subpopulations (NF200, CGRP, and IB4) as well as transcription factors (ATF3 and activated STAT3) following unilateral sciatic nerve injury. Anat Rec, 301:1618-1627, 2018. (c) 2018 Wiley Periodicals, Inc.

Links

GA16-08508S, research and development project

Name: Zvýšení endogenního regeneračního programu a jeho aktivace zprostředkovaná cytokiny/chemokiny v neuronech ganglií bez spojení s poškozeným nervem (Acronym: ERP)

Investor: Czech Science Foundation

Citovat

DUBOVÝ, Petr, Ilona KLUSÁKOVÁ, Ivana HRADILOVÁ SVÍŽENSKÁ and Marek JOUKAL. Expression of Regeneration-Associated Proteins in Primary Sensory Neurons and Regenerating Axons After Nerve Injury-An Overview. ANATOMICAL RECORD-ADVANCES IN INTEGRATIVE ANATOMY AND EVOLUTIONARY BIOLOGY. HOBOKEN: WILEY, 2018, vol. 301, No 10, p. 1618-1627. ISSN 1932-8486. Available from: https://dx.doi.org/10.1002/ar.23843.

@article{1473323,
   author = {Dubový, Petr and Klusáková, Ilona and Hradilová Svíženská, Ivana and Joukal, Marek},
   article_location = {HOBOKEN},
   article_number = {10},
   doi = {http://dx.doi.org/10.1002/ar.23843},
   keywords = {peripheral nerve; regeneration; degeneration; therapies},
   language = {eng},
   issn = {1932-8486},
   journal = {ANATOMICAL RECORD-ADVANCES IN INTEGRATIVE ANATOMY AND EVOLUTIONARY BIOLOGY},
   title = {Expression of Regeneration-Associated Proteins in Primary Sensory Neurons and Regenerating Axons After Nerve Injury-An Overview},
   volume = {301},
   year = {2018}
}

TY  - JOUR
ID  - 1473323
AU  - Dubový, Petr - Klusáková, Ilona - Hradilová Svíženská, Ivana - Joukal, Marek
PY  - 2018
TI  - Expression of Regeneration-Associated Proteins in Primary Sensory Neurons and Regenerating Axons After Nerve Injury-An Overview
JF  - ANATOMICAL RECORD-ADVANCES IN INTEGRATIVE ANATOMY AND EVOLUTIONARY BIOLOGY
VL  - 301
IS  - 10
SP  - 1618-1627
EP  - 1618-1627
PB  - WILEY
SN  - 19328486
KW  - peripheral nerve
KW  - regeneration
KW  - degeneration
KW  - therapies
N2  - Peripheral nerve injury results in profound alterations of the affected neurons resulting from the interplay between intrinsic and extrinsic molecular events. Restarting the neuronal regenerative program is an important prerequisite for functional recovery of the injured peripheral nerve. The primary sensory neurons with their cell bodies in the dorsal root ganglia provide a useful in vivo and in vitro model for studying the mechanisms that regulate intrinsic neuronal regeneration capacity following axotomy. These studies frequently need to indicate the regenerative status of the corresponding neurons. We summarize the critical issues regarding immunohistochemical detection of several regeneration-associated proteins as markers for the initiation of the regeneration program in rat primary sensory neurons and indicators of axon regeneration in the peripheral nerves. This overview also includes our own results of GAP43 and SCG10 expression in different DRG neurons following double immunostaining with molecular markers of neuronal subpopulations (NF200, CGRP, and IB4) as well as transcription factors (ATF3 and activated STAT3) following unilateral sciatic nerve injury. Anat Rec, 301:1618-1627, 2018. (c) 2018 Wiley Periodicals, Inc.
ER  -

DUBOVÝ, Petr, Ilona KLUSÁKOVÁ, Ivana HRADILOVÁ SVÍŽENSKÁ and Marek JOUKAL. Expression of Regeneration-Associated Proteins in Primary Sensory Neurons and Regenerating Axons After Nerve Injury-An Overview. \textit{ANATOMICAL RECORD-ADVANCES IN INTEGRATIVE ANATOMY AND EVOLUTIONARY BIOLOGY}. HOBOKEN: WILEY, 2018, vol.~301, No~10, p.~1618-1627. ISSN~1932-8486. Available from: https://dx.doi.org/10.1002/ar.23843.

Detailed Information on Publication Record