Subarachnoid hemorrhage induces reaction of M1 and M2
macrophages in choroid plexus

a 2018

Subarachnoid hemorrhage induces reaction of M1 and M2 macrophages in choroid plexus

SOLÁR, Peter; Ilona KLUSÁKOVÁ; Radim JANČÁLEK; Petr DUBOVÝ; Marek JOUKAL et. al.

Basic information

Original name

Subarachnoid hemorrhage induces reaction of M1 and M2 macrophages in choroid plexus

Authors

SOLÁR, Peter (703 Slovakia); Ilona KLUSÁKOVÁ (203 Czech Republic); Radim JANČÁLEK (203 Czech Republic); Petr DUBOVÝ (203 Czech Republic) and Marek JOUKAL (203 Czech Republic, guarantor, belonging to the institution)

Edition

Morphology 2018, 2018

Other information

Language

English

Type of outcome

Conference abstract

Field of Study

30103 Neurosciences

Country of publisher

Czech Republic

Confidentiality degree

is not subject to a state or trade secret

RIV identification code

RIV/00216224:14110/18:00104813

Organization unit

Faculty of Medicine

ISBN

978-80-88064-35-0

Keywords (in Czech)

subarachnoidální krvácení; krevně-likvorová bariéra; plexus choroideus; CP; SAH

Keywords in English

subarachnoidal hemorrhage; blood-CSF barrier; plexus choroideus

Changed: 6/12/2018 12:49, doc. MUDr. Marek Joukal, Ph.D.

Abstract

In the original language

Introduction and purpose: The subarachnoid hemorrhage (SAH) is a specific form of the hemorrhagic stroke. Choroid plexus (CP) of the brain ventricles forms the blood-cerebrospinal fluid barrier (B-CSF-B) and is responsible for CSF production. The aim of our study was to describe the cellular changes characterized by the presence of pro-inflammatory (M1) macrophages and anti-inflammatory (M2) macrophages. Material and methods: Wistar rats (n=17; male; 250-300g) were used in experiment. SAH was induced by injection of autologous arterial blood (SAH group) or artificial CSF (control group) into the cisterna magna and rats were left to survive for 1, 3 and 7 days. After time of survival the SAH and control groups of animals were sacrificed in CO2, perfused transcardially by Zamboni´s fixative and the brain was removed. Coronal cryostat sections through the brain were cut and immunohistochemical detection of CCR7+ (M1) and CD206+ (M2) macrophages was performed. Numbers of M1+and M2+ cells per 1 mm2 of CP were counted and statistically analyzed. Results and conclusion: Number of M1+ macrophages was significantly increased 3 days after SAH comparing to naive and control rats. SAH induced significant increased number of M2+ macrophages after 3 and 7 days comparing to naive and control animals. Significant increased number of M2+ macrophages was found 7 days after injection of artificial CSF. The CP responds to SAH with initial increased number of M2+and M2+ macrophages 3 days after SAH. A later anti-inflammatory reaction characterized by presence of M2+ macrophages may contribute to restoration of B-CSF-B after SAH.

Links

MUNI/A/0908/2017, interní kód MU

Name: Reakce plexus choroideus laboratorního potkana na subarachnoidální krvácení

Investor: Masaryk University, Category A

Cite

SOLÁR, Peter; Ilona KLUSÁKOVÁ; Radim JANČÁLEK; Petr DUBOVÝ and Marek JOUKAL. Subarachnoid hemorrhage induces reaction of M1 and M2 macrophages in choroid plexus. In Morphology 2018. 2018. ISBN 978-80-88064-35-0.

@proceedings{1475476,
   author = {Solár, Peter and Klusáková, Ilona and Jančálek, Radim and Dubový, Petr and Joukal, Marek},
   booktitle = {Morphology 2018},
   keywords = {subarachnoidal hemorrhage; blood-CSF barrier; plexus choroideus},
   language = {eng},
   isbn = {978-80-88064-35-0},
   title = {Subarachnoid hemorrhage induces reaction of M1 and M2 macrophages in choroid plexus},
   year = {2018}
}

TY  - CONF
ID  - 1475476
AU  - Solár, Peter - Klusáková, Ilona - Jančálek, Radim - Dubový, Petr - Joukal, Marek
PY  - 2018
TI  - Subarachnoid hemorrhage induces reaction of M1 and M2 macrophages in choroid plexus
SN  - 9788088064350
KW  - subarachnoidal hemorrhage
KW  - blood-CSF barrier
KW  - plexus choroideus
N2  - Introduction and purpose: The subarachnoid hemorrhage (SAH) is a specific form of the hemorrhagic stroke. Choroid plexus (CP) of the brain ventricles forms the blood-cerebrospinal fluid barrier (B-CSF-B) and is responsible for CSF production. The aim of our study was to describe the cellular changes characterized by the presence of pro-inflammatory (M1) macrophages and anti-inflammatory (M2) macrophages. Material and methods: Wistar rats (n=17; male; 250-300g) were used in experiment. SAH was induced by injection of autologous arterial blood (SAH group) or artificial CSF (control group) into the cisterna magna and rats were left to survive for 1, 3 and 7 days. After time of survival the SAH and control groups of animals were sacrificed in CO2, perfused transcardially by Zamboni´s fixative and the brain was removed. Coronal cryostat sections through the brain were cut and immunohistochemical detection of CCR7+ (M1) and CD206+ (M2) macrophages was performed. Numbers of M1+and M2+ cells per 1 mm2 of CP were counted and statistically analyzed. Results and conclusion: Number of M1+ macrophages was significantly increased 3 days after SAH comparing to naive and control rats. SAH induced significant increased number of M2+ macrophages after 3 and 7 days comparing to naive and control animals. Significant increased number of M2+ macrophages was found 7 days after injection of artificial CSF. The CP responds to SAH with initial increased number of M2+and M2+ macrophages 3 days after SAH. A later anti-inflammatory reaction characterized by presence of M2+ macrophages may contribute to restoration of B-CSF-B after SAH.
ER  -

SOLÁR, Peter; Ilona KLUSÁKOVÁ; Radim JANČÁLEK; Petr DUBOVÝ and Marek JOUKAL. Subarachnoid hemorrhage induces reaction of M1 and M2 macrophages in choroid plexus. In \textit{Morphology 2018}. 2018. ISBN~978-80-88064-35-0.

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