KAUROV, Losif, Marie VANCOVÁ, Bernd SCHIMANSKI, Lawrence Rudy CADENA, Jiří HELLER, Tomáš BÍLÝ, David POTĚŠIL, Caludia EICHENBERGER, Hannah BRUCE, Silke OELJAKLAUS, Bettina WARSCHEID, Zbyněk ZDRÁHAL, Andre SCHNEIDER, Julius LUKEŠ and Hassan HASHIMI. The Diverged Trypanosome MICOS Complex as a Hub for Mitochondrial Cristae Shaping and Protein Import. Current Biology. Elsevier Science, vol. 28, No 21, p. 3393-3407, 20 pp. ISSN 0960-9822. doi:10.1016/j.cub.2018.09.008. 2018.
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Basic information
Original name The Diverged Trypanosome MICOS Complex as a Hub for Mitochondrial Cristae Shaping and Protein Import
Authors KAUROV, Losif (203 Czech Republic), Marie VANCOVÁ (203 Czech Republic), Bernd SCHIMANSKI (756 Switzerland), Lawrence Rudy CADENA (203 Czech Republic), Jiří HELLER (203 Czech Republic), Tomáš BÍLÝ (203 Czech Republic), David POTĚŠIL (203 Czech Republic, belonging to the institution), Caludia EICHENBERGER (756 Switzerland), Hannah BRUCE (826 United Kingdom of Great Britain and Northern Ireland), Silke OELJAKLAUS (276 Germany), Bettina WARSCHEID (276 Germany), Zbyněk ZDRÁHAL (203 Czech Republic, guarantor, belonging to the institution), Andre SCHNEIDER (756 Switzerland), Julius LUKEŠ (203 Czech Republic) and Hassan HASHIMI (203 Czech Republic).
Edition Current Biology, Elsevier Science, 2018, 0960-9822.
Other information
Original language English
Type of outcome Article in a journal
Field of Study 10608 Biochemistry and molecular biology
Country of publisher United States of America
Confidentiality degree is not subject to a state or trade secret
Impact factor Impact factor: 9.193
RIV identification code RIV/00216224:14740/18:00104829
Organization unit Central European Institute of Technology
Doi http://dx.doi.org/10.1016/j.cub.2018.09.008
UT WoS 000449621400022
Keywords in English CONTACT SITE; ORGANIZING SYSTEM; INTERMEMBRANE SPACE; INNER MEMBRANE; OUTER-MEMBRANE; MIA PATHWAY; BRUCEI; ORGANIZATION; ARCHITECTURE; BIOGENESIS
Tags CF PROT, rivok
Tags International impact, Reviewed
Changed by Changed by: Mgr. Pavla Foltynová, Ph.D., učo 106624. Changed: 13/3/2019 17:08.
Abstract
The mitochondrial contact site and cristae organization system (MICOS) is a multiprotein complex responsible for cristae formation. Even though cristae are found in all mitochondria capable of oxidative phosphorylation, only Mic10 and Mic60 appear to be conserved throughout eukaryotes. The remaining 4 or 5 known MICOS subunits are specific to the supergroup Opisthokonta, which includes yeast and mammals that are the only organisms in which this complex has been analyzed experimentally. We have isolated the MICOS from Trypanosoma brucei, a member of the supergroup Excavata that is profoundly diverged from opisthokonts. We show that it is required for the maintenance of the unique discoidal cristae that typify excavates, such as euglenids and kinetoplastids, the latter of which include trypanosomes. The trypanosome MICOS consists of 9 subunits, most of which are essential for normal growth. Unlike in opisthokonts, it contains two distinct Mic10 orthologs and an unconventional putative Mic60 that lacks a mitofilin domain. Interestingly, one of the essential trypanosomatid-specific MICOS subunits called TbMic20 is a thioredoxin-like protein that appears to be involved in import of intermembrane space proteins, including respiratory chain complex assembly factors. This result points to trypanosome MICOS coordinating cristae shaping and population of its membrane with proteins involved in respiration, the latter via the catalytic activity of TbMic20. Thus, trypanosome MICOS allows us to define which of its features are conserved in all eukaryotes and decipher those that represent lineage-specific adaptations.
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LM2015043, research and development projectName: Česká infrastruktura pro integrativní strukturní biologii (Acronym: CIISB)
Investor: Ministry of Education, Youth and Sports of the CR
LM2015062, research and development projectName: Národní infrastruktura pro biologické a medicínské zobrazování
Investor: Ministry of Education, Youth and Sports of the CR
LQ1601, research and development projectName: CEITEC 2020 (Acronym: CEITEC2020)
Investor: Ministry of Education, Youth and Sports of the CR
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