Dynamics of the Pollen Sequestrome Defined by Subcellular
Coupled Omics

J 2018

Dynamics of the Pollen Sequestrome Defined by Subcellular Coupled Omics

HAFIDH, Said, David POTĚŠIL, Karel MULLER, Jan FÍLA, Christos MICHAILIDIS et. al.

Basic information

Original name

Dynamics of the Pollen Sequestrome Defined by Subcellular Coupled Omics

Authors

HAFIDH, Said (203 Czech Republic), David POTĚŠIL (203 Czech Republic, belonging to the institution), Karel MULLER (203 Czech Republic), Jan FÍLA (203 Czech Republic), Christos MICHAILIDIS (203 Czech Republic), Anna HERRMANNOVÁ (203 Czech Republic), Jana FECÍKOVÁ (203 Czech Republic), Till ISCHEBECK (276 Germany), Leoš Shivaya VALÁŠEK (203 Czech Republic), Zbyněk ZDRÁHAL (203 Czech Republic, guarantor, belonging to the institution) and David HONYS

Edition

Plant Physiology, American Society of Plant Physiologists, 2018, 0032-0889

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10611 Plant sciences, botany

Country of publisher

United States of America

Confidentiality degree

není předmětem státního či obchodního tajemství

Impact factor

Impact factor: 6.305

RIV identification code

RIV/00216224:14740/18:00101408

Organization unit

Central European Institute of Technology

DOI

http://dx.doi.org/10.1104/pp.18.00648

UT WoS

000444004100022

Keywords in English

DIFFERENTIALLY EXPRESSED PROTEINS; MALE GAMETOPHYTE DEVELOPMENT; MESSENGER-RNA TRANSPORT; TUBE GROWTH; NICOTIANA-TABACUM; GENE-EXPRESSION; ARABIDOPSIS-THALIANA; MATURE POLLEN; PROTEOMIC ANALYSIS; PROCESSING BODIES

Abstract

V originále

Reproduction success in angiosperm plants depends on robust pollen tube growth through the female pistil tissues to ensure successful fertilization. Accordingly, there is an apparent evolutionary trend to accumulate significant reserves during pollen maturation, including a population of stored mRNAs, that are utilized later for a massive translation of various proteins in growing pollen tubes. Here, we performed a thorough transcriptomic and proteomic analysis of stored and translated transcripts in three subcellular compartments of tobacco (Nicotiana tabacum), long-term storage EDTA/puromycin-resistant particles, translating polysomes, and free ribonuclear particles, throughout tobacco pollen development and in in vitro-growing pollen tubes. We demonstrated that the composition of the aforementioned complexes is not rigid and that numerous transcripts were redistributed among these complexes during pollen development, which may represent an important mechanism of translational regulation. Therefore, we defined the pollen sequestrome as a distinct and highly dynamic compartment for the storage of stable, translationally repressed transcripts and demonstrated its dynamics. We propose that EDTA/puromycin-resistant particle complexes represent aggregated nontranslating monosomes as the primary mediators of messenger RNA sequestration. Such organization is extremely useful in fast tip-growing pollen tubes, where rapid and orchestrated protein synthesis must take place in specific regions.

Links

GA18-02448S, research and development project

Name: Úloha translačních iniciačních faktorů při skladování a aktivaci transkriptů v samčí zárodečné linii krytosemenných rostlin

Investor: Czech Science Foundation

LM2015043, research and development project

Name: Česká infrastruktura pro integrativní strukturní biologii (Acronym: CIISB)

Investor: Ministry of Education, Youth and Sports of the CR

LM2015085, research and development project

Name: CERIT Scientific Cloud (Acronym: CERIT-SC)

Investor: Ministry of Education, Youth and Sports of the CR, CERIT Scientific Cloud

LQ1601, research and development project

Name: CEITEC 2020 (Acronym: CEITEC2020)

Investor: Ministry of Education, Youth and Sports of the CR

Citovat

HAFIDH, Said, David POTĚŠIL, Karel MULLER, Jan FÍLA, Christos MICHAILIDIS, Anna HERRMANNOVÁ, Jana FECÍKOVÁ, Till ISCHEBECK, Leoš Shivaya VALÁŠEK, Zbyněk ZDRÁHAL and David HONYS. Dynamics of the Pollen Sequestrome Defined by Subcellular Coupled Omics. Plant Physiology. American Society of Plant Physiologists, 2018, vol. 178, No 1, p. 258-282. ISSN 0032-0889. Available from: https://dx.doi.org/10.1104/pp.18.00648.

@article{1475805,
   author = {Hafidh, Said and Potěšil, David and Muller, Karel and Fíla, Jan and Michailidis, Christos and Herrmannová, Anna and Fecíková, Jana and Ischebeck, Till and Valášek, Leoš Shivaya and Zdráhal, Zbyněk and Honys, David},
   article_number = {1},
   doi = {http://dx.doi.org/10.1104/pp.18.00648},
   keywords = {DIFFERENTIALLY EXPRESSED PROTEINS; MALE GAMETOPHYTE DEVELOPMENT; MESSENGER-RNA TRANSPORT; TUBE GROWTH; NICOTIANA-TABACUM; GENE-EXPRESSION; ARABIDOPSIS-THALIANA; MATURE POLLEN; PROTEOMIC ANALYSIS; PROCESSING BODIES},
   language = {eng},
   issn = {0032-0889},
   journal = {Plant Physiology},
   title = {Dynamics of the Pollen Sequestrome Defined by Subcellular Coupled Omics},
   volume = {178},
   year = {2018}
}

TY  - JOUR
ID  - 1475805
AU  - Hafidh, Said - Potěšil, David - Muller, Karel - Fíla, Jan - Michailidis, Christos - Herrmannová, Anna - Fecíková, Jana - Ischebeck, Till - Valášek, Leoš Shivaya - Zdráhal, Zbyněk - Honys, David
PY  - 2018
TI  - Dynamics of the Pollen Sequestrome Defined by Subcellular Coupled Omics
JF  - Plant Physiology
VL  - 178
IS  - 1
SP  - 258-282
EP  - 258-282
PB  - American Society of Plant Physiologists
SN  - 00320889
KW  - DIFFERENTIALLY EXPRESSED PROTEINS
KW  - MALE GAMETOPHYTE DEVELOPMENT
KW  - MESSENGER-RNA TRANSPORT
KW  - TUBE GROWTH
KW  - NICOTIANA-TABACUM
KW  - GENE-EXPRESSION
KW  - ARABIDOPSIS-THALIANA
KW  - MATURE POLLEN
KW  - PROTEOMIC ANALYSIS
KW  - PROCESSING BODIES
N2  - Reproduction success in angiosperm plants depends on robust pollen tube growth through the female pistil tissues to ensure successful fertilization. Accordingly, there is an apparent evolutionary trend to accumulate significant reserves during pollen maturation, including a population of stored mRNAs, that are utilized later for a massive translation of various proteins in growing pollen tubes. Here, we performed a thorough transcriptomic and proteomic analysis of stored and translated transcripts in three subcellular compartments of tobacco (Nicotiana tabacum), long-term storage EDTA/puromycin-resistant particles, translating polysomes, and free ribonuclear particles, throughout tobacco pollen development and in in vitro-growing pollen tubes. We demonstrated that the composition of the aforementioned complexes is not rigid and that numerous transcripts were redistributed among these complexes during pollen development, which may represent an important mechanism of translational regulation. Therefore, we defined the pollen sequestrome as a distinct and highly dynamic compartment for the storage of stable, translationally repressed transcripts and demonstrated its dynamics. We propose that EDTA/puromycin-resistant particle complexes represent aggregated nontranslating monosomes as the primary mediators of messenger RNA sequestration. Such organization is extremely useful in fast tip-growing pollen tubes, where rapid and orchestrated protein synthesis must take place in specific regions.
ER  -

HAFIDH, Said, David POTĚŠIL, Karel MULLER, Jan FÍLA, Christos MICHAILIDIS, Anna HERRMANNOVÁ, Jana FECÍKOVÁ, Till ISCHEBECK, Leoš Shivaya VALÁŠEK, Zbyněk ZDRÁHAL and David HONYS. Dynamics of the Pollen Sequestrome Defined by Subcellular Coupled Omics. \textit{Plant Physiology}. American Society of Plant Physiologists, 2018, vol.~178, No~1, p.~258-282. ISSN~0032-0889. Available from: https://dx.doi.org/10.1104/pp.18.00648.

Detailed Information on Publication Record