Limitations of routine MALDI-TOF mass spectrometric
identification of Acinetobacter species and remedial actions

J 2018

Limitations of routine MALDI-TOF mass spectrometric identification of Acinetobacter species and remedial actions

ŠEDO, Ondrej, Lenka RADOLFOVÁ-KŘÍŽOVÁ, Alexandr NEMEC and Zbyněk ZDRÁHAL

Basic information

Original name

Limitations of routine MALDI-TOF mass spectrometric identification of Acinetobacter species and remedial actions

Authors

ŠEDO, Ondrej (203 Czech Republic, belonging to the institution), Lenka RADOLFOVÁ-KŘÍŽOVÁ, Alexandr NEMEC and Zbyněk ZDRÁHAL (203 Czech Republic, guarantor, belonging to the institution)

Edition

Journal of Microbiological Methods, Elsevier, 2018, 0167-7012

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10606 Microbiology

Country of publisher

Netherlands

Confidentiality degree

není předmětem státního či obchodního tajemství

Impact factor

Impact factor: 1.803

RIV identification code

RIV/00216224:14740/18:00101425

Organization unit

Central European Institute of Technology

DOI

http://dx.doi.org/10.1016/j.mimet.2018.10.009

UT WoS

000451490900013

Keywords in English

Acinetobacter spp.; Bacteria profiling; BioTyper; MALDI-TOF MS

Abstract

V originále

A set of 204 taxonomically well-defined strains belonging to 17 Acinetobacter spp., including 11 recently described species (A. albensis, A. bohemicus, A. colistiniresistens, A. courvalinii, A. dispersus, A. gandensis, A. modestus, A. proteolyticus, A. seifertii, A. variabilis, and A. vivianii) and six species of the so-called haemolytic Glade (A. beijerinckii, A. gyllenbergii, A. haemolyticus, A. junii, A. parvus, and A. venetianus), were subjected to MALDI-TOF mass spectrometric profiling. The identification outputs were evaluated using the current version (8.0.0.0) of the commercially available Bruker Daltonics, Biotyper database, which does not contain reference entries for six of the species tested. Up to 29% of the strains were falsely identified as different Acinetobacter spp. present in the Biotyper database, resulting mostly from the close phylogenetic relationship of species of the haemolytic Glade. To obtain more reliable identification, extending the commercial database showed only partial improvement, while the use of an alternative MALDI matrix solution (strongly acidified ferulic acid) allowed correct identification of nearly all problematic strains.

Links

EF16_013/0001776, research and development project

Name: Česká infrastruktura pro integrativní strukturní biologii pro lidské zdraví

GBP206/12/G151, research and development project

Name: Centrum nových přístupů k bioanalýze a molekulární diagnostice

LM2015043, research and development project

Name: Česká infrastruktura pro integrativní strukturní biologii (Acronym: CIISB)

Investor: Ministry of Education, Youth and Sports of the CR

LQ1601, research and development project

Name: CEITEC 2020 (Acronym: CEITEC2020)

Investor: Ministry of Education, Youth and Sports of the CR

Citovat

ŠEDO, Ondrej, Lenka RADOLFOVÁ-KŘÍŽOVÁ, Alexandr NEMEC and Zbyněk ZDRÁHAL. Limitations of routine MALDI-TOF mass spectrometric identification of Acinetobacter species and remedial actions. Journal of Microbiological Methods. Elsevier, 2018, vol. 154, NOV 2018, p. 79-85. ISSN 0167-7012. Available from: https://dx.doi.org/10.1016/j.mimet.2018.10.009.

@article{1477346,
   author = {Šedo, Ondrej and RadolfováandKřížová, Lenka and Nemec, Alexandr and Zdráhal, Zbyněk},
   article_number = {NOV 2018},
   doi = {http://dx.doi.org/10.1016/j.mimet.2018.10.009},
   keywords = {Acinetobacter spp.; Bacteria profiling; BioTyper; MALDI-TOF MS},
   language = {eng},
   issn = {0167-7012},
   journal = {Journal of Microbiological Methods},
   title = {Limitations of routine MALDI-TOF mass spectrometric identification of Acinetobacter species and remedial actions},
   volume = {154},
   year = {2018}
}

TY  - JOUR
ID  - 1477346
AU  - Šedo, Ondrej - Radolfová-Křížová, Lenka - Nemec, Alexandr - Zdráhal, Zbyněk
PY  - 2018
TI  - Limitations of routine MALDI-TOF mass spectrometric identification of Acinetobacter species and remedial actions
JF  - Journal of Microbiological Methods
VL  - 154
IS  - NOV 2018
SP  - 79-85
EP  - 79-85
PB  - Elsevier
SN  - 01677012
KW  - Acinetobacter spp.
KW  - Bacteria profiling
KW  - BioTyper
KW  - MALDI-TOF MS
N2  - A set of 204 taxonomically well-defined strains belonging to 17 Acinetobacter spp., including 11 recently described species (A. albensis, A. bohemicus, A. colistiniresistens, A. courvalinii, A. dispersus, A. gandensis, A. modestus, A. proteolyticus, A. seifertii, A. variabilis, and A. vivianii) and six species of the so-called haemolytic Glade (A. beijerinckii, A. gyllenbergii, A. haemolyticus, A. junii, A. parvus, and A. venetianus), were subjected to MALDI-TOF mass spectrometric profiling. The identification outputs were evaluated using the current version (8.0.0.0) of the commercially available Bruker Daltonics, Biotyper database, which does not contain reference entries for six of the species tested. Up to 29% of the strains were falsely identified as different Acinetobacter spp. present in the Biotyper database, resulting mostly from the close phylogenetic relationship of species of the haemolytic Glade. To obtain more reliable identification, extending the commercial database showed only partial improvement, while the use of an alternative MALDI matrix solution (strongly acidified ferulic acid) allowed correct identification of nearly all problematic strains.
ER  -

ŠEDO, Ondrej, Lenka RADOLFOVÁ-KŘÍŽOVÁ, Alexandr NEMEC and Zbyněk ZDRÁHAL. Limitations of routine MALDI-TOF mass spectrometric identification of Acinetobacter species and remedial actions. \textit{Journal of Microbiological Methods}. Elsevier, 2018, vol.~154, NOV 2018, p.~79-85. ISSN~0167-7012. Available from: https://dx.doi.org/10.1016/j.mimet.2018.10.009.

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