Multiplex Assay for Quantification of Acute Phase Proteins and
Immunoglobulin A in Dried Blood Spots

J 2019

Multiplex Assay for Quantification of Acute Phase Proteins and Immunoglobulin A in Dried Blood Spots

VIDOVÁ, Veronika, Eliška STUCHLÍKOVÁ, Markéta VRBOVÁ, Martina ALMÁŠI, Jana KLÁNOVÁ et. al.

Basic information

Original name

Multiplex Assay for Quantification of Acute Phase Proteins and Immunoglobulin A in Dried Blood Spots

Authors

VIDOVÁ, Veronika (203 Czech Republic, belonging to the institution), Eliška STUCHLÍKOVÁ (203 Czech Republic, belonging to the institution), Markéta VRBOVÁ (203 Czech Republic, belonging to the institution), Martina ALMÁŠI (203 Czech Republic), Jana KLÁNOVÁ (203 Czech Republic, belonging to the institution), Vojtěch THON (203 Czech Republic, belonging to the institution) and Zdeněk SPÁČIL (203 Czech Republic, guarantor, belonging to the institution)

Edition

Journal of Proteome Research, Washington, USA, AMER CHEMICAL SOC, 2019, 1535-3893

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10609 Biochemical research methods

Country of publisher

United States of America

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

Full Text

Impact factor

Impact factor: 4.074

RIV identification code

RIV/00216224:14310/19:00107209

Organization unit

Faculty of Science

DOI

http://dx.doi.org/10.1021/acs.jproteome.8b00657

UT WoS

000455285900034

Keywords in English

inflammation markers; acute phase proteins; immune response; dried blood spots; targeted quantitative proteomics; selected reaction monitoring

Abstract

V originále

Inflammation is the first line defense mechanism against infection, tissue damage, or cancer development. However, inappropriate inflammatory response may also trigger diseases. The quantification of inflammatory proteins is essential to distinguish between harmful and beneficial immune response. Currently used immunoanalytical assays may suffer specificity issues due to antigen-antibody interaction and possible cross-reactivity of antibody with other protein species. In addition, immunoanalytical assays typically require invasive blood sampling and additional logistics; they are relatively costly and highly challenging to multiplex. We present a multiplex assay based on selected reaction monitoring (SRM) for quantification of seven acute-phase proteins (i.e., SAA1, SAA2-isoform1, SAA4, CRP, A1AT-isoform1, A1AG1, A1AG2) and the adaptive immunity effector IGHA1 in dried blood spots. This type of sample is readily available from all human subjects including newborns. The study utilizes proteotypic isotopically labeled peptides with trypsin-cleavable tag and presents optimized and reproducible workflow and several important practical remarks regarding quantitative SRM assays development. The panel of inflammatory proteins was quantified with sequence specificity capable to differentiate protein isoforms with intra- and interday precision (<16.4% coefficient of variation (CV) and <14.3% CV, respectively). Quantitative results were correlated with immuno-nephelometric assay (typically greater than 0.9 Pearson's R).

Links

EF15_003/0000469, research and development project

Name: Cetocoen Plus

GJ17-24592Y, research and development project

Name: Mapování interakcí mezi základními metabolickými pochody a střevní mikroflórou

Investor: Czech Science Foundation

LM2015051, research and development project

Name: Centrum pro výzkum toxických látek v prostředí (Acronym: RECETOX RI)

Investor: Ministry of Education, Youth and Sports of the CR

Citovat

VIDOVÁ, Veronika, Eliška STUCHLÍKOVÁ, Markéta VRBOVÁ, Martina ALMÁŠI, Jana KLÁNOVÁ, Vojtěch THON and Zdeněk SPÁČIL. Multiplex Assay for Quantification of Acute Phase Proteins and Immunoglobulin A in Dried Blood Spots. Journal of Proteome Research. Washington, USA: AMER CHEMICAL SOC, 2019, vol. 18, No 1, p. 380-391. ISSN 1535-3893. Available from: https://dx.doi.org/10.1021/acs.jproteome.8b00657.

@article{1479457,
   author = {Vidová, Veronika and Stuchlíková, Eliška and Vrbová, Markéta and Almáši, Martina and Klánová, Jana and Thon, Vojtěch and Spáčil, Zdeněk},
   article_location = {Washington, USA},
   article_number = {1},
   doi = {http://dx.doi.org/10.1021/acs.jproteome.8b00657},
   keywords = {inflammation markers; acute phase proteins; immune response; dried blood spots; targeted quantitative proteomics; selected reaction monitoring},
   language = {eng},
   issn = {1535-3893},
   journal = {Journal of Proteome Research},
   title = {Multiplex Assay for Quantification of Acute Phase Proteins and Immunoglobulin A in Dried Blood Spots},
   url = {https://pubs.acs.org/doi/10.1021/acs.jproteome.8b00657},
   volume = {18},
   year = {2019}
}

TY  - JOUR
ID  - 1479457
AU  - Vidová, Veronika - Stuchlíková, Eliška - Vrbová, Markéta - Almáši, Martina - Klánová, Jana - Thon, Vojtěch - Spáčil, Zdeněk
PY  - 2019
TI  - Multiplex Assay for Quantification of Acute Phase Proteins and Immunoglobulin A in Dried Blood Spots
JF  - Journal of Proteome Research
VL  - 18
IS  - 1
SP  - 380-391
EP  - 380-391
PB  - AMER CHEMICAL SOC
SN  - 15353893
KW  - inflammation markers
KW  - acute phase proteins
KW  - immune response
KW  - dried blood spots
KW  - targeted quantitative proteomics
KW  - selected reaction monitoring
UR  - https://pubs.acs.org/doi/10.1021/acs.jproteome.8b00657
L2  - https://pubs.acs.org/doi/10.1021/acs.jproteome.8b00657
N2  - Inflammation is the first line defense mechanism against infection, tissue damage, or cancer development. However, inappropriate inflammatory response may also trigger diseases. The quantification of inflammatory proteins is essential to distinguish between harmful and beneficial immune response. Currently used immunoanalytical assays may suffer specificity issues due to antigen-antibody interaction and possible cross-reactivity of antibody with other protein species. In addition, immunoanalytical assays typically require invasive blood sampling and additional logistics; they are relatively costly and highly challenging to multiplex. We present a multiplex assay based on selected reaction monitoring (SRM) for quantification of seven acute-phase proteins (i.e., SAA1, SAA2-isoform1, SAA4, CRP, A1AT-isoform1, A1AG1, A1AG2) and the adaptive immunity effector IGHA1 in dried blood spots. This type of sample is readily available from all human subjects including newborns. The study utilizes proteotypic isotopically labeled peptides with trypsin-cleavable tag and presents optimized and reproducible workflow and several important practical remarks regarding quantitative SRM assays development. The panel of inflammatory proteins was quantified with sequence specificity capable to differentiate protein isoforms with intra- and interday precision (<16.4% coefficient of variation (CV) and <14.3% CV, respectively). Quantitative results were correlated with immuno-nephelometric assay (typically greater than 0.9 Pearson's R).
ER  -

VIDOVÁ, Veronika, Eliška STUCHLÍKOVÁ, Markéta VRBOVÁ, Martina ALMÁŠI, Jana KLÁNOVÁ, Vojtěch THON and Zdeněk SPÁČIL. Multiplex Assay for Quantification of Acute Phase Proteins and Immunoglobulin A in Dried Blood Spots. \textit{Journal of Proteome Research}. Washington, USA: AMER CHEMICAL SOC, 2019, vol.~18, No~1, p.~380-391. ISSN~1535-3893. Available from: https://dx.doi.org/10.1021/acs.jproteome.8b00657.

Detailed Information on Publication Record