Aim: To present the validation process of the data standard for the collection of data on pressure ulcers and, in particular, to identify the necessary and unnecessary items that should be monitored at the local level of the health service providers. On the contrary, the aim is not to compare the number of records of pressure ulcers and occurrence of pressure ulcers, but to assess the possibility of data collection in clinical practice and to assess their usability in relation to the measures necessary for the provision of care. Methodology: Second pilot validation of the dataset was carried out in three participating university hospitals (centres) in the Czech Republic for a period of 6 months (2nd half of 2017). The first pre-pilot validation was organised in the previous 6 months. Statistical analysis of data was performed in SPSS (IBM Corp., Armonk, NY, USA) at a significance level of 0.05. Results: In total, data from 320 records of patients were submitted from all three centres, with 310 completed history records and 498 records of pressure ulcers. There were no statistically significant differences in data availability and completion of the standardized data set in the required structure (p > 0.05). Based on an assessment of the administrative burden and the clinical benefit of the data, changes were made to the final data standard. Conclusion: The prepared technical description of the standardized and validated dataset together with the technical description of the software will allow simple implementation at national level to unify the special dataset for monitoring of pressure ulcers.