Nuclear hormone receptors: Ancient 9aaTAD and evolutionally
gained NCoA activation pathways

J 2019

Nuclear hormone receptors: Ancient 9aaTAD and evolutionally gained NCoA activation pathways

PISKÁČEK, Martin, Marek HAVELKA, Kristína JENDRUCHOVÁ and Andrea KNIGHT

Basic information

Original name

Nuclear hormone receptors: Ancient 9aaTAD and evolutionally gained NCoA activation pathways

Authors

PISKÁČEK, Martin (40 Austria, belonging to the institution), Marek HAVELKA (203 Czech Republic, belonging to the institution), Kristína JENDRUCHOVÁ (703 Slovakia, belonging to the institution) and Andrea KNIGHT (203 Czech Republic, belonging to the institution)

Edition

Journal of steroid biochemistry and molecular biology, Oxford, Pergamon-Elsevier Science, 2019, 0960-0760

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10608 Biochemistry and molecular biology

Country of publisher

United Kingdom of Great Britain and Northern Ireland

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

URL

Impact factor

Impact factor: 3.813

RIV identification code

RIV/00216224:14110/19:00108447

Organization unit

Faculty of Medicine

DOI

http://dx.doi.org/10.1016/j.jsbmb.2018.11.008

UT WoS

000459952100015

Keywords in English

9aaTAD; CBP; HNF4; KIX; MED15; PPAR; RARa; VDL

Abstract

V originále

In higher metazoans, the nuclear hormone receptors activate transcription trough their specific adaptors, nuclear hormone receptor adaptors NCoA, which are absent in lower metazoans. The Nine amino acid TransActivation Domain, 9aaTAD, was reported for a large number of the transcription activators that recruit general mediators of transcription. In this study, we demonstrated that the 9aaTAD from NHR-49 receptor of nematode C.elegans activates transcription as a small peptide. We showed that the ancient 9aaTAD domains are conserved in the nuclear hormone receptors including human HNF4, RARa, VDR and PPARg. Also their small 9aaTAD peptides effectively activated transcription in absence of the NCoA adaptors. We also showed that adjacent H11 domains in ancient and modern hormone receptors have an inhibitory effect on their 9aaTAD function.

Links

NV15-32935A, research and development project

Name: Analýza gamma-delta T lymfocytů reaktivních na nádorové buňky u pacientů s B-buněčnou chronickou lymfocytární leukémií: nový přístup k buněčné terapii

Citovat

PISKÁČEK, Martin, Marek HAVELKA, Kristína JENDRUCHOVÁ and Andrea KNIGHT. Nuclear hormone receptors: Ancient 9aaTAD and evolutionally gained NCoA activation pathways. Journal of steroid biochemistry and molecular biology. Oxford: Pergamon-Elsevier Science, 2019, vol. 187, MAR 2019, p. 118-123. ISSN 0960-0760. Available from: https://dx.doi.org/10.1016/j.jsbmb.2018.11.008.

@article{1479856,
   author = {Piskáček, Martin and Havelka, Marek and Jendruchová, Kristína and Knight, Andrea},
   article_location = {Oxford},
   article_number = {MAR 2019},
   doi = {http://dx.doi.org/10.1016/j.jsbmb.2018.11.008},
   keywords = {9aaTAD; CBP; HNF4; KIX; MED15; PPAR; RARa; VDL},
   language = {eng},
   issn = {0960-0760},
   journal = {Journal of steroid biochemistry and molecular biology},
   title = {Nuclear hormone receptors: Ancient 9aaTAD and evolutionally gained NCoA activation pathways},
   url = {http://dx.doi.org/10.1016/j.jsbmb.2018.11.008},
   volume = {187},
   year = {2019}
}

TY  - JOUR
ID  - 1479856
AU  - Piskáček, Martin - Havelka, Marek - Jendruchová, Kristína - Knight, Andrea
PY  - 2019
TI  - Nuclear hormone receptors: Ancient 9aaTAD and evolutionally gained NCoA activation pathways
JF  - Journal of steroid biochemistry and molecular biology
VL  - 187
IS  - MAR 2019
SP  - 118-123
EP  - 118-123
PB  - Pergamon-Elsevier Science
SN  - 09600760
KW  - 9aaTAD
KW  - CBP
KW  - HNF4
KW  - KIX
KW  - MED15
KW  - PPAR
KW  - RARa
KW  - VDL
UR  - http://dx.doi.org/10.1016/j.jsbmb.2018.11.008
L2  - http://dx.doi.org/10.1016/j.jsbmb.2018.11.008
N2  - In higher metazoans, the nuclear hormone receptors activate transcription trough their specific adaptors, nuclear hormone receptor adaptors NCoA, which are absent in lower metazoans. The Nine amino acid TransActivation Domain, 9aaTAD, was reported for a large number of the transcription activators that recruit general mediators of transcription. In this study, we demonstrated that the 9aaTAD from NHR-49 receptor of nematode C.elegans activates transcription as a small peptide. We showed that the ancient 9aaTAD domains are conserved in the nuclear hormone receptors including human HNF4, RARa, VDR and PPARg. Also their small 9aaTAD peptides effectively activated transcription in absence of the NCoA adaptors. We also showed that adjacent H11 domains in ancient and modern hormone receptors have an inhibitory effect on their 9aaTAD function.
ER  -

PISKÁČEK, Martin, Marek HAVELKA, Kristína JENDRUCHOVÁ and Andrea KNIGHT. Nuclear hormone receptors: Ancient 9aaTAD and evolutionally gained NCoA activation pathways. \textit{Journal of steroid biochemistry and molecular biology}. Oxford: Pergamon-Elsevier Science, 2019, vol.~187, MAR 2019, p.~118-123. ISSN~0960-0760. Available from: https://dx.doi.org/10.1016/j.jsbmb.2018.11.008.

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