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@inbook{1480517,
author = {Schejbal, Jan and Řemínek, Roman and Glatz, Zdeněk},
address = {USA},
booktitle = {Targeting Enzymes for Pharmaceutical Development: Methods and Protocols},
doi = {http://dx.doi.org/10.1007/978-1-0716-0163-1_9},
edition = {1},
editor = {Nikolaos Labrou, PhD},
keywords = {Capillary electrophoresis; Mass spectrometry; Alzheimer’s disease; Beta-secretase; Enzyme inhibition; Screening},
howpublished = {tištěná verze "print"},
language = {eng},
location = {USA},
isbn = {978-1-0716-0162-4},
pages = {147-157},
publisher = {Springer},
title = {Screening of Beta-Secretase Inhibitors by Capillary Electrophoresis-Mass Spectrometry},
url = {https://link.springer.com/protocol/10.1007%2F978-1-0716-0163-1_9},
year = {2020}
}
TY - CHAP
ID - 1480517
AU - Schejbal, Jan - Řemínek, Roman - Glatz, Zdeněk
PY - 2020
TI - Screening of Beta-Secretase Inhibitors by Capillary Electrophoresis-Mass Spectrometry
VL - Targeting Enzymes for Pharmaceutical Development: Methods and Protocols 2089
PB - Springer
CY - USA
SN - 9781071601624
KW - Capillary electrophoresis
KW - Mass spectrometry
KW - Alzheimer’s disease
KW - Beta-secretase
KW - Enzyme inhibition
KW - Screening
UR - https://link.springer.com/protocol/10.1007%2F978-1-0716-0163-1_9
N2 - Alzheimer’s disease is the most common cause of dementia, currently afflicting almost 40 million patients worldwide. According to the amyloid cascade hypothesis, the pathogenesis of the disease could be slowed down or even stopped by the inhibition of beta-secretase, making this aspartic-acid protease a potentially important drug target site. Capillary electrophoresis is a promising technique for screening putative enzyme inhibitors due to highly effective separations, minuscule sample and other chemicals consumption, compatibility with a variety of detection techniques, and high-throughput via automation. This chapter presents a method based on capillary electrophoresis coupled to mass spectrometry detection for kinetic and inhibition assays of the beta-secretase reaction with a decapeptide derived from an amyloid precursor protein.
ER -
SCHEJBAL, Jan, Roman ŘEMÍNEK and Zdeněk GLATZ. Screening of Beta-Secretase Inhibitors by Capillary Electrophoresis-Mass Spectrometry. In Nikolaos Labrou, PhD. \textit{Targeting Enzymes for Pharmaceutical Development: Methods and Protocols}. 1st ed. USA: Springer, 2020, p.~147-157. Targeting Enzymes for Pharmaceutical Development: Methods and Protocols 2089. ISBN~978-1-0716-0162-4. Available from: https://dx.doi.org/10.1007/978-1-0716-0163-1\_{}9.
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