Detailed Information on Publication Record
2020
Online Enantioselective Capillary Electrophoretic Method for Screening Cytochrome P450 3A4 Inhibitors
ŘEMÍNEK, Roman, Zdeněk GLATZ and Wolfgang THORMANNBasic information
Original name
Online Enantioselective Capillary Electrophoretic Method for Screening Cytochrome P450 3A4 Inhibitors
Name in Czech
Online enantioselektivní kapilárně elektroforetická metoda pro screening inhibitorů cytochromu P450 3A4
Authors
ŘEMÍNEK, Roman (203 Czech Republic, belonging to the institution), Zdeněk GLATZ (203 Czech Republic, guarantor, belonging to the institution) and Wolfgang THORMANN (756 Switzerland)
Edition
1. vyd. USA, Targeting Enzymes for Pharmaceutical Development: Methods and Protocols, p. 167-178, 12 pp. Targeting Enzymes for Pharmaceutical Development, 2020
Publisher
Springer
Other information
Language
English
Type of outcome
Kapitola resp. kapitoly v odborné knize
Field of Study
10609 Biochemical research methods
Country of publisher
United States of America
Confidentiality degree
není předmětem státního či obchodního tajemství
Publication form
printed version "print"
References:
RIV identification code
RIV/00216224:14310/20:00113958
Organization unit
Faculty of Science
ISBN
978-1-0716-0162-4
UT WoS
000650966200012
Keywords (in Czech)
Kapilární elektroforéza; Enantioselektivní separace; Cytochrom P450; CYP3A4; Ketamin
Keywords in English
Capillary electrophoresis; Enantioselective separation; Cytochrome P450; CYP3A4; Ketamine
Tags
Tags
International impact, Reviewed
Změněno: 16/8/2021 12:46, Mgr. Marie Šípková, DiS.
V originále
The market share of single-enantiomer drugs is steadily increasing. The pharmacodynamics and pharmacokinetics of individual enantiomers can differ considerably. Thus, their characteristics have to be addressed as early as possible in the development process of new pharmaceuticals. Capillary electrophoresis is a promising technique for enantioselective drug metabolism studies due to highly effective separations, minuscule consumption of sample and reagents, compatibility with a variety of detection techniques, high-throughput via automation, and the implementation of on-line procedures. An on-line method comprised of the diffusion-based mixing of cytochrome P450 3A4 with racemic ketamine, incubation of the enzyme reaction, separation of the reaction products S- and R-norketamine and their quantification is presented in this chapter. Since diffusion is an inherent property of all molecules, the method enables the addition of virtually any compound to the reaction mixture without the need for additional optimization of the mixing conditions, and thus can be favorably used for the rapid screening of putative cytochrome P450 3A4 inhibitors.
In Czech
Byl publikován protokol pro screening inhibitorů cytochromu P450 3A4 pomocí on-line enantioselektivní metody založené na kapilární elektroforéze.
Links
GA16-06106S, research and development project |
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