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@inbook{1482480,
author = {Řemínek, Roman and Glatz, Zdeněk and Thormann, Wolfgang},
address = {USA},
booktitle = {Targeting Enzymes for Pharmaceutical Development: Methods and Protocols},
doi = {http://dx.doi.org/10.1007/978-1-0716-0163-1_11},
edition = {1.},
editor = {N.E. Labrou},
keywords = {Capillary electrophoresis; Enantioselective separation; Cytochrome P450; CYP3A4; Ketamine},
howpublished = {tištěná verze "print"},
language = {eng},
location = {USA},
isbn = {978-1-0716-0162-4},
pages = {167-178},
publisher = {Springer},
title = {Online Enantioselective Capillary Electrophoretic Method for Screening Cytochrome P450 3A4 Inhibitors},
url = {https://link.springer.com/protocol/10.1007%2F978-1-0716-0163-1_11},
year = {2020}
}
TY - CHAP
ID - 1482480
AU - Řemínek, Roman - Glatz, Zdeněk - Thormann, Wolfgang
PY - 2020
TI - Online Enantioselective Capillary Electrophoretic Method for Screening Cytochrome P450 3A4 Inhibitors
VL - Targeting Enzymes for Pharmaceutical Development
PB - Springer
CY - USA
SN - 9781071601624
KW - Capillary electrophoresis
KW - Enantioselective separation
KW - Cytochrome P450
KW - CYP3A4
KW - Ketamine
UR - https://link.springer.com/protocol/10.1007%2F978-1-0716-0163-1_11
N2 - The market share of single-enantiomer drugs is steadily increasing. The pharmacodynamics and pharmacokinetics of individual enantiomers can differ considerably. Thus, their characteristics have to be addressed as early as possible in the development process of new pharmaceuticals. Capillary electrophoresis is a promising technique for enantioselective drug metabolism studies due to highly effective separations, minuscule consumption of sample and reagents, compatibility with a variety of detection techniques, high-throughput via automation, and the implementation of on-line procedures. An on-line method comprised of the diffusion-based mixing of cytochrome P450 3A4 with racemic ketamine, incubation of the enzyme reaction, separation of the reaction products S- and R-norketamine and their quantification is presented in this chapter. Since diffusion is an inherent property of all molecules, the method enables the addition of virtually any compound to the reaction mixture without the need for additional optimization of the mixing conditions, and thus can be favorably used for the rapid screening of putative cytochrome P450 3A4 inhibitors.
ER -
ŘEMÍNEK, Roman, Zdeněk GLATZ and Wolfgang THORMANN. Online Enantioselective Capillary Electrophoretic Method for Screening Cytochrome P450 3A4 Inhibitors. In N.E. Labrou. \textit{Targeting Enzymes for Pharmaceutical Development: Methods and Protocols}. 1st ed. USA: Springer, 2020, p.~167-178. Targeting Enzymes for Pharmaceutical Development. ISBN~978-1-0716-0162-4. Available from: https://dx.doi.org/10.1007/978-1-0716-0163-1\_{}11.
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