The planar cell polarity protein VANG-1/Vangl negatively
regulates Wnt/beta-catenin signaling through a Dvl dependent
mechanism

J 2018

The planar cell polarity protein VANG-1/Vangl negatively regulates Wnt/beta-catenin signaling through a Dvl dependent mechanism

MENTINK, R.A., L. RELLA, Tomasz Witold RADASZKIEWICZ, Tomáš GYBEĽ, M.C. BETIST et. al.

Basic information

Original name

The planar cell polarity protein VANG-1/Vangl negatively regulates Wnt/beta-catenin signaling through a Dvl dependent mechanism

Authors

MENTINK, R.A. (528 Netherlands), L. RELLA (528 Netherlands), Tomasz Witold RADASZKIEWICZ (616 Poland, belonging to the institution), Tomáš GYBEĽ (703 Slovakia, belonging to the institution), M.C. BETIST (528 Netherlands), Vítězslav BRYJA (203 Czech Republic, guarantor, belonging to the institution) and H.C. KORSWAGEN (528 Netherlands)

Edition

PLoS Genetics, San Francisco, Public Library of Science, 2018, 1553-7404

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10601 Cell biology

Country of publisher

United States of America

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

URL

Impact factor

Impact factor: 5.224

RIV identification code

RIV/00216224:14310/18:00101505

Organization unit

Faculty of Science

DOI

http://dx.doi.org/10.1371/journal.pgen.1007840

UT WoS

000455099000023

Keywords in English

CANONICAL WNT PATHWAY; C. ELEGANS; NEUROBLAST MIGRATION; GENE-EXPRESSION; VANGL2; GASTRULATION; MUTATIONS; DIVISION; AXIS; PHOSPHORYLATION

Abstract

V originále

Van Gogh-like (Vangl) and Prickle (Pk) are core components of the non-canonical Wnt planar cell polarity pathway that controls epithelial polarity and cell migration. Studies in vertebrate model systems have suggested that Vangl and Pk may also inhibit signaling through the canonical Wnt/beta-catenin pathway, but the functional significance of this potential crosstalk is unclear. In the nematode C. elegans, the Q neuroblasts and their descendants migrate in opposite directions along the anteroposterior body axis. The direction of these migrations is specified by Wnt signaling, with activation of canonical Wnt signaling driving posterior migration, and non-canonical Wnt signaling anterior migration. Here, we show that the Vangl ortholog VANG-1 influences the Wnt signaling response of the Q neuroblasts by negatively regulating canonical Wnt signaling. This inhibitory activity depends on a carboxyterminal PDZ binding motif in VANG-1 and the Dishevelled ortholog MIG-5, but is independent of the Pk ortholog PRKL-1. Moreover, using Vangl1 and Vangl2 double mutant cells, we show that a similar mechanism acts in mammalian cells. We conclude that cross-talk between VANG-1/Vangl and the canonical Wnt pathway is an evolutionarily conserved mechanism that ensures robust specification of Wnt signaling responses.

Links

GA17-16680S, research and development project

Name: Nové postupy pro určení aktivity dráhy planární buněčné polarity (PCP)

Investor: Czech Science Foundation

GA18-17658S, research and development project

Name: Odhalení tajemství signální dráhy WNT analýzou struktury proteinu Dishevelled

Investor: Czech Science Foundation

Citovat

MENTINK, R.A., L. RELLA, Tomasz Witold RADASZKIEWICZ, Tomáš GYBEĽ, M.C. BETIST, Vítězslav BRYJA and H.C. KORSWAGEN. The planar cell polarity protein VANG-1/Vangl negatively regulates Wnt/beta-catenin signaling through a Dvl dependent mechanism. PLoS Genetics. San Francisco: Public Library of Science, 2018, vol. 14, No 12, p. 1-22. ISSN 1553-7404. Available from: https://dx.doi.org/10.1371/journal.pgen.1007840.

@article{1483381,
   author = {Mentink, R.A. and Rella, L. and Radaszkiewicz, Tomasz Witold and Gybeľ, Tomáš and Betist, M.C. and Bryja, Vítězslav and Korswagen, H.C.},
   article_location = {San Francisco},
   article_number = {12},
   doi = {http://dx.doi.org/10.1371/journal.pgen.1007840},
   keywords = {CANONICAL WNT PATHWAY; C. ELEGANS; NEUROBLAST MIGRATION; GENE-EXPRESSION; VANGL2; GASTRULATION; MUTATIONS; DIVISION; AXIS; PHOSPHORYLATION},
   language = {eng},
   issn = {1553-7404},
   journal = {PLoS Genetics},
   title = {The planar cell polarity protein VANG-1/Vangl negatively regulates Wnt/beta-catenin signaling through a Dvl dependent mechanism},
   url = {https://journals.plos.org/plosgenetics/article?id=10.1371/journal.pgen.1007840},
   volume = {14},
   year = {2018}
}

TY  - JOUR
ID  - 1483381
AU  - Mentink, R.A. - Rella, L. - Radaszkiewicz, Tomasz Witold - Gybeľ, Tomáš - Betist, M.C. - Bryja, Vítězslav - Korswagen, H.C.
PY  - 2018
TI  - The planar cell polarity protein VANG-1/Vangl negatively regulates Wnt/beta-catenin signaling through a Dvl dependent mechanism
JF  - PLoS Genetics
VL  - 14
IS  - 12
SP  - 1-22
EP  - 1-22
PB  - Public Library of Science
SN  - 15537404
KW  - CANONICAL WNT PATHWAY
KW  - C. ELEGANS
KW  - NEUROBLAST MIGRATION
KW  - GENE-EXPRESSION
KW  - VANGL2
KW  - GASTRULATION
KW  - MUTATIONS
KW  - DIVISION
KW  - AXIS
KW  - PHOSPHORYLATION
UR  - https://journals.plos.org/plosgenetics/article?id=10.1371/journal.pgen.1007840
L2  - https://journals.plos.org/plosgenetics/article?id=10.1371/journal.pgen.1007840
N2  - Van Gogh-like (Vangl) and Prickle (Pk) are core components of the non-canonical Wnt planar cell polarity pathway that controls epithelial polarity and cell migration. Studies in vertebrate model systems have suggested that Vangl and Pk may also inhibit signaling through the canonical Wnt/beta-catenin pathway, but the functional significance of this potential crosstalk is unclear. In the nematode C. elegans, the Q neuroblasts and their descendants migrate in opposite directions along the anteroposterior body axis. The direction of these migrations is specified by Wnt signaling, with activation of canonical Wnt signaling driving posterior migration, and non-canonical Wnt signaling anterior migration. Here, we show that the Vangl ortholog VANG-1 influences the Wnt signaling response of the Q neuroblasts by negatively regulating canonical Wnt signaling. This inhibitory activity depends on a carboxyterminal PDZ binding motif in VANG-1 and the Dishevelled ortholog MIG-5, but is independent of the Pk ortholog PRKL-1. Moreover, using Vangl1 and Vangl2 double mutant cells, we show that a similar mechanism acts in mammalian cells. We conclude that cross-talk between VANG-1/Vangl and the canonical Wnt pathway is an evolutionarily conserved mechanism that ensures robust specification of Wnt signaling responses.
ER  -

MENTINK, R.A., L. RELLA, Tomasz Witold RADASZKIEWICZ, Tomáš GYBEĽ, M.C. BETIST, Vítězslav BRYJA and H.C. KORSWAGEN. The planar cell polarity protein VANG-1/Vangl negatively regulates Wnt/beta-catenin signaling through a Dvl dependent mechanism. \textit{PLoS Genetics}. San Francisco: Public Library of Science, 2018, vol.~14, No~12, p.~1-22. ISSN~1553-7404. Available from: https://dx.doi.org/10.1371/journal.pgen.1007840.

Detailed Information on Publication Record