Capillary electrophoresis-based approaches for the study of
affinity interactions combined with various sensitive and
non-traditional detection techniques

J 2019

Capillary electrophoresis-based approaches for the study of affinity interactions combined with various sensitive and non-traditional detection techniques

NEVÍDALOVÁ, Hana, Lenka MICHALCOVÁ and Zdeněk GLATZ

Basic information

Original name

Capillary electrophoresis-based approaches for the study of affinity interactions combined with various sensitive and non-traditional detection techniques

Name in Czech

Metody studia afinitních interakcí založené na CE v kombinaci s různými typy detekce

Authors

NEVÍDALOVÁ, Hana (203 Czech Republic, belonging to the institution), Lenka MICHALCOVÁ (203 Czech Republic, belonging to the institution) and Zdeněk GLATZ (203 Czech Republic, guarantor, belonging to the institution)

Edition

Electrophoresis, Hoboken, Wiley, 2019, 0173-0835

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10600 1.6 Biological sciences

Country of publisher

United States of America

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

Full Text

Impact factor

Impact factor: 3.081

RIV identification code

RIV/00216224:14310/19:00107222

Organization unit

Faculty of Science

DOI

http://dx.doi.org/10.1002/elps.201800367

UT WoS

000460305100004

Keywords (in Czech)

afinitní interakce; CE; LIF; MSů čipová CE.

Keywords in English

Affinity interaction; capillary electrophoresis; fluorescence detection; mass spectrometry; microchip capillary electrophoresis.

Abstract

V originále

Nearly all processes in living organisms are controlled and regulated by the synergy of many biomolecule interactions involving proteins, peptides, nucleic acids, nucleotides, saccharides and small molecular weight ligands. There is growing interest in understanding them, not only for the purposes of interactomics as an essential part of system biology, but also in their further elucidation in disease pathology, diagnostics and treatment. The necessity of detailed investigation of these interactions leads to the requirement of laboratory methods characterised by high efficiency and sensitivity. As a result, many instrumental approaches differing in their fundamental principles have been developed, including those based on capillary electrophoresis. Although capillary electrophoresis offers numerous advantages for such studies, it still has one serious limitation, its poor concentration sensitivity with the most commonly used detection method – ultraviolet-visible spectrometry. However, coupling capillary electrophoresis with a more sensitive detector fulfils the above-mentioned requirement. In this review, capillary electrophoresis combined with fluorescence, mass spectrometry and several non-traditional detection techniques in affinity interaction studies are summarised and discussed, together with the possibility of conducting these measurements in microchip format.

Links

GA16-06106S, research and development project

Name: Vysoce efektivní systém založený na kapilární elektroforéze pro screening inhibitorů beta-sekretázy jako terapeutického cíle pro Alzheimerovu chorobu (Acronym: Alzheimer)

Investor: Czech Science Foundation

GA19-08358S, research and development project

Name: Nové přístupy pro studium afinitních interakcí založené na kapilární elektroforéze

Investor: Czech Science Foundation

MUNI/A/1278/2016, interní kód MU

Name: Podpora biochemického výzkumu v roce 2017

Investor: Masaryk University, Category A

Citovat

NEVÍDALOVÁ, Hana, Lenka MICHALCOVÁ and Zdeněk GLATZ. Capillary electrophoresis-based approaches for the study of affinity interactions combined with various sensitive and non-traditional detection techniques. Electrophoresis. Hoboken: Wiley, 2019, vol. 40, No 5, p. 625-642. ISSN 0173-0835. Available from: https://dx.doi.org/10.1002/elps.201800367.

@article{1483892,
   author = {Nevídalová, Hana and Michalcová, Lenka and Glatz, Zdeněk},
   article_location = {Hoboken},
   article_number = {5},
   doi = {http://dx.doi.org/10.1002/elps.201800367},
   keywords = {Affinity interaction; capillary electrophoresis; fluorescence detection; mass spectrometry; microchip capillary electrophoresis.},
   language = {eng},
   issn = {0173-0835},
   journal = {Electrophoresis},
   title = {Capillary electrophoresis-based approaches for the study of affinity interactions combined with various sensitive and non-traditional detection techniques},
   url = {https://onlinelibrary.wiley.com/doi/full/10.1002/elps.201800367},
   volume = {40},
   year = {2019}
}

TY  - JOUR
ID  - 1483892
AU  - Nevídalová, Hana - Michalcová, Lenka - Glatz, Zdeněk
PY  - 2019
TI  - Capillary electrophoresis-based approaches for the study of affinity interactions combined with various sensitive and non-traditional detection techniques
JF  - Electrophoresis
VL  - 40
IS  - 5
SP  - 625-642
EP  - 625-642
PB  - Wiley
SN  - 01730835
KW  - Affinity interaction
KW  - capillary electrophoresis
KW  - fluorescence detection
KW  - mass spectrometry
KW  - microchip capillary electrophoresis.
UR  - https://onlinelibrary.wiley.com/doi/full/10.1002/elps.201800367
L2  - https://onlinelibrary.wiley.com/doi/full/10.1002/elps.201800367
N2  - Nearly all processes in living organisms are controlled and regulated by the synergy of many biomolecule interactions involving proteins, peptides, nucleic acids, nucleotides, saccharides and small molecular weight ligands. There is growing interest in understanding them, not only for the purposes of interactomics as an essential part of system biology, but also in their further elucidation in disease pathology, diagnostics and treatment. The necessity of detailed investigation of these interactions leads to the requirement of laboratory methods characterised by high efficiency and sensitivity. As a result, many instrumental approaches differing in their fundamental principles have been developed, including those based on capillary electrophoresis. Although capillary electrophoresis offers numerous advantages for such studies, it still has one serious limitation, its poor concentration sensitivity with the most commonly used detection method – ultraviolet-visible spectrometry. However, coupling capillary electrophoresis with a more sensitive detector fulfils the above-mentioned requirement. In this review, capillary electrophoresis combined with fluorescence, mass spectrometry and several non-traditional detection techniques in affinity interaction studies are summarised and discussed, together with the possibility of conducting these measurements in microchip format.
ER  -

NEVÍDALOVÁ, Hana, Lenka MICHALCOVÁ and Zdeněk GLATZ. Capillary electrophoresis-based approaches for the study of affinity interactions combined with various sensitive and non-traditional detection techniques. \textit{Electrophoresis}. Hoboken: Wiley, 2019, vol.~40, No~5, p.~625-642. ISSN~0173-0835. Available from: https://dx.doi.org/10.1002/elps.201800367.

Detailed Information on Publication Record