Identification and partial characterization of a novel serpin
from Eudiplozoon nipponicum (Monogenea, Polyopisthocotylea)

J 2018

Identification and partial characterization of a novel serpin from Eudiplozoon nipponicum (Monogenea, Polyopisthocotylea)

ROUDNICKÝ, Pavel, Jiří VOREL, Jana ILGOVÁ, Michal BENOVICS, Adam NOREK et. al.

Basic information

Original name

Identification and partial characterization of a novel serpin from Eudiplozoon nipponicum (Monogenea, Polyopisthocotylea)

Authors

ROUDNICKÝ, Pavel (203 Czech Republic, guarantor, belonging to the institution), Jiří VOREL (203 Czech Republic, belonging to the institution), Jana ILGOVÁ (703 Slovakia, belonging to the institution), Michal BENOVICS (703 Slovakia, belonging to the institution), Adam NOREK (203 Czech Republic), Lucie JEDLIČKOVÁ (203 Czech Republic), Libor MIKEŠ (203 Czech Republic), David POTĚŠIL (203 Czech Republic, belonging to the institution), Zbyněk ZDRÁHAL (203 Czech Republic, belonging to the institution), Jan DVOŘÁK (203 Czech Republic), Milan GELNAR (203 Czech Republic, belonging to the institution) and Martin KAŠNÝ (203 Czech Republic, belonging to the institution)

Edition

Parasite, 2018, 1252-607X

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10613 Zoology

Country of publisher

France

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

URL

Impact factor

Impact factor: 1.958

RIV identification code

RIV/00216224:14310/18:00101521

Organization unit

Faculty of Science

DOI

http://dx.doi.org/10.1051/parasite/2018062

UT WoS

000452194000001

Keywords in English

Eudiplozoon nipponicum; fish parasite; monogenea; Platyhelminths; serpin; inhibitor

Abstract

V originále

Background: Serpins are a superfamily of serine peptidase inhibitors that participate in the regulation of many physiological and cell peptidase-mediated processes in all organisms (e.g. in blood clotting, complement activation, fibrinolysis, inflammation, and programmed cell death). It was postulated that in the blood-feeding members of the monogenean family Diplozoidae, serpins could play an important role in the prevention of thrombus formation, activation of complement, inflammation in the host, and/or in the endogenous regulation of protein degradation. Results: In silico analysis showed that the DNA and primary protein structures of serpin from Eudiplozoon nipponicum (EnSerp1) are similar to other members of the serpin superfamily. The inhibitory potential of EnSerp1 on four physiologically-relevant serine peptidases (trypsin, factor Xa, kallikrein, and plasmin) was demonstrated and its presence in the worm’s excretory-secretory products (ESPs) was confirmed. Conclusion: EnSerp1 influences the activity of peptidases that play a role in blood coagulation, fibrinolysis, and complement activation. This inhibitory potential, together with the serpin’s presence in ESPs, suggests that it is likely involved in host-parasite interactions and could be one of the molecules involved in the control of feeding and prevention of inflammatory responses.

Links

GAP506/12/1258, research and development project

Name: Interakce hostitel-parazit u krevsajících diplozoidních monogeneí: Výzkum vysoce specializovaných adaptací k parazitismu

Investor: Czech Science Foundation

GBP505/12/G112, research and development project

Name: ECIP - Evropské centrum ichtyoparazitologie

Investor: Czech Science Foundation

LM2015043, research and development project

Name: Česká infrastruktura pro integrativní strukturní biologii (Acronym: CIISB)

Investor: Ministry of Education, Youth and Sports of the CR

LM2015085, research and development project

Name: CERIT Scientific Cloud (Acronym: CERIT-SC)

Investor: Ministry of Education, Youth and Sports of the CR, CERIT Scientific Cloud

MUNI/A/1362/2016, interní kód MU

Name: Biologická diverzita – analýzy biologických systémů různých úrovní a na různých škálách prostředí

Investor: Masaryk University, Category A

Citovat

ROUDNICKÝ, Pavel, Jiří VOREL, Jana ILGOVÁ, Michal BENOVICS, Adam NOREK, Lucie JEDLIČKOVÁ, Libor MIKEŠ, David POTĚŠIL, Zbyněk ZDRÁHAL, Jan DVOŘÁK, Milan GELNAR and Martin KAŠNÝ. Identification and partial characterization of a novel serpin from Eudiplozoon nipponicum (Monogenea, Polyopisthocotylea). Parasite. 2018, vol. 25, DEC, p. "61", 13 pp. ISSN 1252-607X. Available from: https://dx.doi.org/10.1051/parasite/2018062.

@article{1484114,
   author = {Roudnický, Pavel and Vorel, Jiří and Ilgová, Jana and Benovics, Michal and Norek, Adam and Jedličková, Lucie and Mikeš, Libor and Potěšil, David and Zdráhal, Zbyněk and Dvořák, Jan and Gelnar, Milan and Kašný, Martin},
   article_number = {DEC},
   doi = {http://dx.doi.org/10.1051/parasite/2018062},
   keywords = {Eudiplozoon nipponicum; fish parasite; monogenea; Platyhelminths; serpin; inhibitor},
   language = {eng},
   issn = {1252-607X},
   journal = {Parasite},
   title = {Identification and partial characterization of a novel serpin from Eudiplozoon nipponicum (Monogenea, Polyopisthocotylea)},
   url = {https://www.parasite-journal.org/articles/parasite/abs/2018/01/parasite180126/parasite180126.html},
   volume = {25},
   year = {2018}
}

TY  - JOUR
ID  - 1484114
AU  - Roudnický, Pavel - Vorel, Jiří - Ilgová, Jana - Benovics, Michal - Norek, Adam - Jedličková, Lucie - Mikeš, Libor - Potěšil, David - Zdráhal, Zbyněk - Dvořák, Jan - Gelnar, Milan - Kašný, Martin
PY  - 2018
TI  - Identification and partial characterization of a novel serpin from Eudiplozoon nipponicum (Monogenea, Polyopisthocotylea)
JF  - Parasite
VL  - 25
IS  - DEC
SP  - "61"
EP  - "61"
SN  - 1252607X
KW  - Eudiplozoon nipponicum
KW  - fish parasite
KW  - monogenea
KW  - Platyhelminths
KW  - serpin
KW  - inhibitor
UR  - https://www.parasite-journal.org/articles/parasite/abs/2018/01/parasite180126/parasite180126.html
L2  - https://www.parasite-journal.org/articles/parasite/abs/2018/01/parasite180126/parasite180126.html
N2  - Background: Serpins are a superfamily of serine peptidase inhibitors that participate in the regulation of many physiological and cell peptidase-mediated processes in all organisms (e.g. in blood clotting, complement activation, fibrinolysis, inflammation, and programmed cell death). It was postulated that in the blood-feeding members of the monogenean family Diplozoidae, serpins could play an important role in the prevention of thrombus formation, activation of complement, inflammation in the host, and/or in the endogenous regulation of protein degradation. Results: In silico analysis showed that the DNA and primary protein structures of serpin from Eudiplozoon nipponicum (EnSerp1) are similar to other members of the serpin superfamily. The inhibitory potential of EnSerp1 on four physiologically-relevant serine peptidases (trypsin, factor Xa, kallikrein, and plasmin) was demonstrated and its presence in the worm’s excretory-secretory products (ESPs) was confirmed. Conclusion: EnSerp1 influences the activity of peptidases that play a role in blood coagulation, fibrinolysis, and complement activation. This inhibitory potential, together with the serpin’s presence in ESPs, suggests that it is likely involved in host-parasite interactions and could be one of the molecules involved in the control of feeding and prevention of inflammatory responses.
ER  -

ROUDNICKÝ, Pavel, Jiří VOREL, Jana ILGOVÁ, Michal BENOVICS, Adam NOREK, Lucie JEDLIČKOVÁ, Libor MIKEŠ, David POTĚŠIL, Zbyněk ZDRÁHAL, Jan DVOŘÁK, Milan GELNAR and Martin KAŠNÝ. Identification and partial characterization of a novel serpin from Eudiplozoon nipponicum (Monogenea, Polyopisthocotylea). \textit{Parasite}. 2018, vol.~25, DEC, p.~''61'', 13 pp. ISSN~1252-607X. Available from: https://dx.doi.org/10.1051/parasite/2018062.

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