Investigation of Detergent-Modified Enzymomimetic Activities of
TEMED-Templated Nanoceria Towards Fluorescent Detection of
Their Cellular Uptake

J 2018

Investigation of Detergent-Modified Enzymomimetic Activities of TEMED-Templated Nanoceria Towards Fluorescent Detection of Their Cellular Uptake

PEŠKOVÁ, Marie, Zbyněk HEGER, Simona DOSTALOVA, Michaela FOJTŮ, Klára CASTKOVA et. al.

Basic information

Original name

Investigation of Detergent-Modified Enzymomimetic Activities of TEMED-Templated Nanoceria Towards Fluorescent Detection of Their Cellular Uptake

Authors

PEŠKOVÁ, Marie (203 Czech Republic, belonging to the institution), Zbyněk HEGER (203 Czech Republic), Simona DOSTALOVA (203 Czech Republic), Michaela FOJTŮ (203 Czech Republic, belonging to the institution), Klára CASTKOVA (203 Czech Republic), Ladislav ILKOVICS (203 Czech Republic, belonging to the institution), Vít VYKOUKAL (203 Czech Republic, belonging to the institution) and Vladimír PEKAŘÍK (203 Czech Republic, guarantor, belonging to the institution)

Edition

ChemistrySelect, WEINHEIM, WILEY-V C H VERLAG GMBH, 2018, 2365-6549

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

30105 Physiology

Country of publisher

Germany

Confidentiality degree

není předmětem státního či obchodního tajemství

Impact factor

Impact factor: 1.716

RIV identification code

RIV/00216224:14110/18:00105316

Organization unit

Faculty of Medicine

DOI

http://dx.doi.org/10.1002/slct.201802496

UT WoS

000445850200003

Keywords in English

Detergents; fluorescent detection; haloperoxidase; nanoceria hydrate; TEMED

Abstract

V originále

Ceria nanoparticles can catalyze many redox reactions that resemble reactions catalyzed by naturally occurring enzymes, such as peroxidase, catalase, haloperoxidase or phosphatase. This makes nanoceria an attractive material for biomedical and sensing applications. Presence of detergents dramatically affects the catalytic activity of nanoceria. The catalytic activity is also strongly influenced by biogenic compounds present in the sample. Furthermore, biomedical applications are hindered by the difficulties to detect nanoceria uptake by cells. Therefore, we have developed a new fast and sensitive fluorescent haloperoxidase assay based on fluorescein bromination in presence of CTAB to detect uptake of CeO2 nanoparticles by cells invitro. Moreover, catalytic activities of standard nanoceria (CeO2) were compared to nanoceria hydrates (CeO2 center dot xH(2)O) that are easier for preparation and might be stabilized by biologically relevant molecules allowing for a specific targeting to cells or tissues. We have developed a new approach to produce CeO2 center dot xH(2)O in presence of TEMED and analyzed whether the presence of H2O2 during the synthesis influences the catalytic activity of produced particles.

Links

LQ1601, research and development project

Name: CEITEC 2020 (Acronym: CEITEC2020)

Investor: Ministry of Education, Youth and Sports of the CR

Citovat

PEŠKOVÁ, Marie, Zbyněk HEGER, Simona DOSTALOVA, Michaela FOJTŮ, Klára CASTKOVA, Ladislav ILKOVICS, Vít VYKOUKAL and Vladimír PEKAŘÍK. Investigation of Detergent-Modified Enzymomimetic Activities of TEMED-Templated Nanoceria Towards Fluorescent Detection of Their Cellular Uptake. ChemistrySelect. WEINHEIM: WILEY-V C H VERLAG GMBH, 2018, vol. 3, No 36, p. 10139-10146. ISSN 2365-6549. Available from: https://dx.doi.org/10.1002/slct.201802496.

@article{1484251,
   author = {Pešková, Marie and Heger, Zbyněk and Dostalova, Simona and Fojtů, Michaela and Castkova, Klára and Ilkovics, Ladislav and Vykoukal, Vít and Pekařík, Vladimír},
   article_location = {WEINHEIM},
   article_number = {36},
   doi = {http://dx.doi.org/10.1002/slct.201802496},
   keywords = {Detergents; fluorescent detection; haloperoxidase; nanoceria hydrate; TEMED},
   language = {eng},
   issn = {2365-6549},
   journal = {ChemistrySelect},
   title = {Investigation of Detergent-Modified Enzymomimetic Activities of TEMED-Templated Nanoceria Towards Fluorescent Detection of Their Cellular Uptake},
   volume = {3},
   year = {2018}
}

TY  - JOUR
ID  - 1484251
AU  - Pešková, Marie - Heger, Zbyněk - Dostalova, Simona - Fojtů, Michaela - Castkova, Klára - Ilkovics, Ladislav - Vykoukal, Vít - Pekařík, Vladimír
PY  - 2018
TI  - Investigation of Detergent-Modified Enzymomimetic Activities of TEMED-Templated Nanoceria Towards Fluorescent Detection of Their Cellular Uptake
JF  - ChemistrySelect
VL  - 3
IS  - 36
SP  - 10139-10146
EP  - 10139-10146
PB  - WILEY-V C H VERLAG GMBH
SN  - 23656549
KW  - Detergents
KW  - fluorescent detection
KW  - haloperoxidase
KW  - nanoceria hydrate
KW  - TEMED
N2  - Ceria nanoparticles can catalyze many redox reactions that resemble reactions catalyzed by naturally occurring enzymes, such as peroxidase, catalase, haloperoxidase or phosphatase. This makes nanoceria an attractive material for biomedical and sensing applications. Presence of detergents dramatically affects the catalytic activity of nanoceria. The catalytic activity is also strongly influenced by biogenic compounds present in the sample. Furthermore, biomedical applications are hindered by the difficulties to detect nanoceria uptake by cells. Therefore, we have developed a new fast and sensitive fluorescent haloperoxidase assay based on fluorescein bromination in presence of CTAB to detect uptake of CeO2 nanoparticles by cells invitro. Moreover, catalytic activities of standard nanoceria (CeO2) were compared to nanoceria hydrates (CeO2 center dot xH(2)O) that are easier for preparation and might be stabilized by biologically relevant molecules allowing for a specific targeting to cells or tissues. We have developed a new approach to produce CeO2 center dot xH(2)O in presence of TEMED and analyzed whether the presence of H2O2 during the synthesis influences the catalytic activity of produced particles.
ER  -

PEŠKOVÁ, Marie, Zbyněk HEGER, Simona DOSTALOVA, Michaela FOJTŮ, Klára CASTKOVA, Ladislav ILKOVICS, Vít VYKOUKAL and Vladimír PEKAŘÍK. Investigation of Detergent-Modified Enzymomimetic Activities of TEMED-Templated Nanoceria Towards Fluorescent Detection of Their Cellular Uptake. \textit{ChemistrySelect}. WEINHEIM: WILEY-V C H VERLAG GMBH, 2018, vol.~3, No~36, p.~10139-10146. ISSN~2365-6549. Available from: https://dx.doi.org/10.1002/slct.201802496.

Detailed Information on Publication Record