Detailed Information on Publication Record
2018
Investigation of Detergent-Modified Enzymomimetic Activities of TEMED-Templated Nanoceria Towards Fluorescent Detection of Their Cellular Uptake
PEŠKOVÁ, Marie, Zbyněk HEGER, Simona DOSTALOVA, Michaela FOJTŮ, Klára CASTKOVA et. al.Basic information
Original name
Investigation of Detergent-Modified Enzymomimetic Activities of TEMED-Templated Nanoceria Towards Fluorescent Detection of Their Cellular Uptake
Authors
PEŠKOVÁ, Marie (203 Czech Republic, belonging to the institution), Zbyněk HEGER (203 Czech Republic), Simona DOSTALOVA (203 Czech Republic), Michaela FOJTŮ (203 Czech Republic, belonging to the institution), Klára CASTKOVA (203 Czech Republic), Ladislav ILKOVICS (203 Czech Republic, belonging to the institution), Vít VYKOUKAL (203 Czech Republic, belonging to the institution) and Vladimír PEKAŘÍK (203 Czech Republic, guarantor, belonging to the institution)
Edition
ChemistrySelect, WEINHEIM, WILEY-V C H VERLAG GMBH, 2018, 2365-6549
Other information
Language
English
Type of outcome
Článek v odborném periodiku
Field of Study
30105 Physiology
Country of publisher
Germany
Confidentiality degree
není předmětem státního či obchodního tajemství
Impact factor
Impact factor: 1.716
RIV identification code
RIV/00216224:14110/18:00105316
Organization unit
Faculty of Medicine
UT WoS
000445850200003
Keywords in English
Detergents; fluorescent detection; haloperoxidase; nanoceria hydrate; TEMED
Tags
International impact, Reviewed
Změněno: 21/3/2019 12:28, Soňa Böhmová
Abstract
V originále
Ceria nanoparticles can catalyze many redox reactions that resemble reactions catalyzed by naturally occurring enzymes, such as peroxidase, catalase, haloperoxidase or phosphatase. This makes nanoceria an attractive material for biomedical and sensing applications. Presence of detergents dramatically affects the catalytic activity of nanoceria. The catalytic activity is also strongly influenced by biogenic compounds present in the sample. Furthermore, biomedical applications are hindered by the difficulties to detect nanoceria uptake by cells. Therefore, we have developed a new fast and sensitive fluorescent haloperoxidase assay based on fluorescein bromination in presence of CTAB to detect uptake of CeO2 nanoparticles by cells invitro. Moreover, catalytic activities of standard nanoceria (CeO2) were compared to nanoceria hydrates (CeO2 center dot xH(2)O) that are easier for preparation and might be stabilized by biologically relevant molecules allowing for a specific targeting to cells or tissues. We have developed a new approach to produce CeO2 center dot xH(2)O in presence of TEMED and analyzed whether the presence of H2O2 during the synthesis influences the catalytic activity of produced particles.
Links
| LQ1601, research and development project |
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