Metabolic responses of Ulva compressa to single and combined
heavy metals

J 2018

Metabolic responses of Ulva compressa to single and combined heavy metals

KOVACIK, Jozef, Giuseppe MICALIZZI, Slawomir DRESLER, Petr BABULA, Juraj HLADKY et. al.

Basic information

Original name

Metabolic responses of Ulva compressa to single and combined heavy metals

Authors

KOVACIK, Jozef (703 Slovakia, guarantor), Giuseppe MICALIZZI (380 Italy), Slawomir DRESLER (616 Poland), Petr BABULA (203 Czech Republic, belonging to the institution), Juraj HLADKY (703 Slovakia), Alexander CHEMODANOV (376 Israel) and Luigi MONDELLO (380 Italy)

Edition

Chemosphere, OXFORD, PERGAMON-ELSEVIER SCIENCE LTD, 2018, 0045-6535

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10511 Environmental sciences

Country of publisher

United Kingdom of Great Britain and Northern Ireland

Confidentiality degree

není předmětem státního či obchodního tajemství

Impact factor

Impact factor: 5.108

RIV identification code

RIV/00216224:14110/18:00105327

Organization unit

Faculty of Medicine

DOI

http://dx.doi.org/10.1016/j.chemosphere.2018.08.141

UT WoS

000448493700042

Keywords in English

Antioxidants; Fluorescence microscopy; Gas chromatography; Heavy metals

Abstract

V originále

Accumulation of metals and metabolic responses were studied for two Cd and Cu concentrations (1 and 10 mu M) either alone or as a combination in marine macroalga after 7 days of exposure. Cd accumulated more at a low dose (115 mu g of Cclig DW) but Cu at a high dose (378 mu g of Cu/g DW); Cu suppressed Cd accumulation (by 57%). Na and Zn levels were unaffected, but higher metal doses depleted K and Ca levels. Higher metal concentrations strongly stimulated reactive oxygen species and depleted nitric oxide (NO) formation, but differences between the action of Cd and Cu were not extensive. Higher metal doses increased cell wall thickness with a potential relation to NO signal that is visible mainly in the apoplast in those treatments. A higher Cu dose depleted proline, ascorbic acid, and phenol levels more than Cd, whereas Cd elevated nonprotein thiols and ascorbic acid in combined treatments. An eventual role of malic or citric acid in metal chelation was not evident: malic acid level decreased in all treatments. The total content of fatty acids reached 16.7 mg/g DW in control with the quantitative order of PUFAs > SFAs > MUFAs; palmitic, vaccenic, linoleic, and alpha-linolenic acids were the major compounds. Cu was more toxic for fatty acids than Cd (even at 1 mu M); mainly, PUFA levels strongly decreased (from 43% of total acids in control to 28.9% and 5.4% at 1 and 10 mu M Cu treatment, respectively). Results are precisely and critically discussed in relation to limited literature focused on macroalgae, and a comparison with microalgae is also provided. (C) 2018 Elsevier Ltd. All rights reserved.

Citovat

KOVACIK, Jozef, Giuseppe MICALIZZI, Slawomir DRESLER, Petr BABULA, Juraj HLADKY, Alexander CHEMODANOV and Luigi MONDELLO. Metabolic responses of Ulva compressa to single and combined heavy metals. Chemosphere. OXFORD: PERGAMON-ELSEVIER SCIENCE LTD, 2018, vol. 213, DEC 2018, p. 384-394. ISSN 0045-6535. Available from: https://dx.doi.org/10.1016/j.chemosphere.2018.08.141.

@article{1484325,
   author = {Kovacik, Jozef and Micalizzi, Giuseppe and Dresler, Slawomir and Babula, Petr and Hladky, Juraj and Chemodanov, Alexander and Mondello, Luigi},
   article_location = {OXFORD},
   article_number = {DEC 2018},
   doi = {http://dx.doi.org/10.1016/j.chemosphere.2018.08.141},
   keywords = {Antioxidants; Fluorescence microscopy; Gas chromatography; Heavy metals},
   language = {eng},
   issn = {0045-6535},
   journal = {Chemosphere},
   title = {Metabolic responses of Ulva compressa to single and combined heavy metals},
   volume = {213},
   year = {2018}
}

TY  - JOUR
ID  - 1484325
AU  - Kovacik, Jozef - Micalizzi, Giuseppe - Dresler, Slawomir - Babula, Petr - Hladky, Juraj - Chemodanov, Alexander - Mondello, Luigi
PY  - 2018
TI  - Metabolic responses of Ulva compressa to single and combined heavy metals
JF  - Chemosphere
VL  - 213
IS  - DEC 2018
SP  - 384-394
EP  - 384-394
PB  - PERGAMON-ELSEVIER SCIENCE LTD
SN  - 00456535
KW  - Antioxidants
KW  - Fluorescence microscopy
KW  - Gas chromatography
KW  - Heavy metals
N2  - Accumulation of metals and metabolic responses were studied for two Cd and Cu concentrations (1 and 10 mu M) either alone or as a combination in marine macroalga after 7 days of exposure. Cd accumulated more at a low dose (115 mu g of Cclig DW) but Cu at a high dose (378 mu g of Cu/g DW); Cu suppressed Cd accumulation (by 57%). Na and Zn levels were unaffected, but higher metal doses depleted K and Ca levels. Higher metal concentrations strongly stimulated reactive oxygen species and depleted nitric oxide (NO) formation, but differences between the action of Cd and Cu were not extensive. Higher metal doses increased cell wall thickness with a potential relation to NO signal that is visible mainly in the apoplast in those treatments. A higher Cu dose depleted proline, ascorbic acid, and phenol levels more than Cd, whereas Cd elevated nonprotein thiols and ascorbic acid in combined treatments. An eventual role of malic or citric acid in metal chelation was not evident: malic acid level decreased in all treatments. The total content of fatty acids reached 16.7 mg/g DW in control with the quantitative order of PUFAs > SFAs > MUFAs; palmitic, vaccenic, linoleic, and alpha-linolenic acids were the major compounds. Cu was more toxic for fatty acids than Cd (even at 1 mu M); mainly, PUFA levels strongly decreased (from 43% of total acids in control to 28.9% and 5.4% at 1 and 10 mu M Cu treatment, respectively). Results are precisely and critically discussed in relation to limited literature focused on macroalgae, and a comparison with microalgae is also provided. (C) 2018 Elsevier Ltd. All rights reserved.
ER  -

KOVACIK, Jozef, Giuseppe MICALIZZI, Slawomir DRESLER, Petr BABULA, Juraj HLADKY, Alexander CHEMODANOV and Luigi MONDELLO. Metabolic responses of Ulva compressa to single and combined heavy metals. \textit{Chemosphere}. OXFORD: PERGAMON-ELSEVIER SCIENCE LTD, 2018, vol.~213, DEC 2018, p.~384-394. ISSN~0045-6535. Available from: https://dx.doi.org/10.1016/j.chemosphere.2018.08.141.

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