| GOLAN, Martin, Jan PŘIBYL, Martin PEŠL, Šárka JELÍNKOVÁ, Ivana AĆIMOVIĆ, Josef JAROŠ, Vladimír ROTREKL, Martin FALK, Ludek SEFC, Petr SKLÁDAL and Irena KRATOCHVILOVA. Cryopreserved Cells Regeneration Monitored by Atomic Force Microscopy and Correlated With State of Cytoskeleton and Nuclear Membrane. IEEE TRANSACTIONS ON NANOBIOSCIENCE. PISCATAWAY: IEEE-INST ELECTRICAL ELECTRONICS ENGINEERS INC, 2018, vol. 17, No 4, p. 485-497. ISSN 1536-1241. Available from: https://dx.doi.org/10.1109/TNB.2018.2873425. |
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@article{1484586,
author = {Golan, Martin and Přibyl, Jan and Pešl, Martin and Jelínková, Šárka and Aćimović, Ivana and Jaroš, Josef and Rotrekl, Vladimír and Falk, Martin and Sefc, Ludek and Skládal, Petr and Kratochvilova, Irena},
article_location = {PISCATAWAY},
article_number = {4},
doi = {http://dx.doi.org/10.1109/TNB.2018.2873425},
keywords = {Atomic forcemicroscopy; cell surface stiffness; cryopreservation; cryopreservedcells reconstruction},
language = {eng},
issn = {1536-1241},
journal = {IEEE TRANSACTIONS ON NANOBIOSCIENCE},
title = {Cryopreserved Cells Regeneration Monitored by Atomic Force Microscopy and Correlated With State of Cytoskeleton and Nuclear Membrane},
volume = {17},
year = {2018}
}
TY - JOUR
ID - 1484586
AU - Golan, Martin - Přibyl, Jan - Pešl, Martin - Jelínková, Šárka - Aćimović, Ivana - Jaroš, Josef - Rotrekl, Vladimír - Falk, Martin - Sefc, Ludek - Skládal, Petr - Kratochvilova, Irena
PY - 2018
TI - Cryopreserved Cells Regeneration Monitored by Atomic Force Microscopy and Correlated With State of Cytoskeleton and Nuclear Membrane
JF - IEEE TRANSACTIONS ON NANOBIOSCIENCE
VL - 17
IS - 4
SP - 485-497
EP - 485-497
PB - IEEE-INST ELECTRICAL ELECTRONICS ENGINEERS INC
SN - 15361241
KW - Atomic forcemicroscopy
KW - cell surface stiffness
KW - cryopreservation
KW - cryopreservedcells reconstruction
N2 - Atomic force microscopy (AFM) helps to describe and explain the mechanobiological properties of living cells on the nanoscale level under physiological conditions. The stiffness of cells is an important parameter reflecting cell physiology. Here, we have provided the first study of the stiffness of cryopreserved cells during post- thawing regeneration using AFM combined with confocal fluorescence microscopy. We demonstrated that the nonfrozen cell stiffness decreased proportionally to the cryoprotectant concentration in the medium. AFM allowed us to map cell surface reconstitution in real time after a freeze/thaw cycle and to monitor the regeneration processes at different depths of the cell and even different parts of the cell surface (nucleus and edge). Fluorescence microscopy showed that the cytoskeleton in fibroblasts, though damaged by the freeze/thaw cycle, is reconstructed after long-termplating. Confocalmicroscopy confirmed that structural changes affect the nuclear envelopes in cryopreserved cells. AFM nanoindentation analysis could be used as a noninvasive method to identify cells that have regenerated their surface mechanical properties with the proper dynamics and to a sufficient degree. This identification can be important particularly in the field of in vitro fertilization and in future cell-based regeneration strategies.
ER -
GOLAN, Martin, Jan PŘIBYL, Martin PEŠL, Šárka JELÍNKOVÁ, Ivana A$\backslash$'CIMOVI$\backslash$'C, Josef JAROŠ, Vladimír ROTREKL, Martin FALK, Ludek SEFC, Petr SKLÁDAL and Irena KRATOCHVILOVA. Cryopreserved Cells Regeneration Monitored by Atomic Force Microscopy and Correlated With State of Cytoskeleton and Nuclear Membrane. \textit{IEEE TRANSACTIONS ON NANOBIOSCIENCE}. PISCATAWAY: IEEE-INST ELECTRICAL ELECTRONICS ENGINEERS INC, 2018, vol.~17, No~4, p.~485-497. ISSN~1536-1241. Available from: https://dx.doi.org/10.1109/TNB.2018.2873425.
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