J 2018

Cryopreserved Cells Regeneration Monitored by Atomic Force Microscopy and Correlated With State of Cytoskeleton and Nuclear Membrane

GOLAN, Martin, Jan PŘIBYL, Martin PEŠL, Šárka JELÍNKOVÁ, Ivana AĆIMOVIĆ et. al.

Basic information

Original name

Cryopreserved Cells Regeneration Monitored by Atomic Force Microscopy and Correlated With State of Cytoskeleton and Nuclear Membrane

Authors

GOLAN, Martin (203 Czech Republic), Jan PŘIBYL (203 Czech Republic, guarantor, belonging to the institution), Martin PEŠL (203 Czech Republic, belonging to the institution), Šárka JELÍNKOVÁ (203 Czech Republic, belonging to the institution), Ivana AĆIMOVIĆ (688 Serbia, belonging to the institution), Josef JAROŠ (203 Czech Republic, belonging to the institution), Vladimír ROTREKL (203 Czech Republic, belonging to the institution), Martin FALK (203 Czech Republic), Ludek SEFC (203 Czech Republic), Petr SKLÁDAL (203 Czech Republic, belonging to the institution) and Irena KRATOCHVILOVA (203 Czech Republic)

Edition

IEEE TRANSACTIONS ON NANOBIOSCIENCE, PISCATAWAY, IEEE-INST ELECTRICAL ELECTRONICS ENGINEERS INC, 2018, 1536-1241

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10609 Biochemical research methods

Country of publisher

United States of America

Confidentiality degree

není předmětem státního či obchodního tajemství

Impact factor

Impact factor: 1.927

RIV identification code

RIV/00216224:14740/18:00101533

Organization unit

Central European Institute of Technology

UT WoS

000450357100015

Keywords in English

Atomic forcemicroscopy; cell surface stiffness; cryopreservation; cryopreservedcells reconstruction

Tags

International impact, Reviewed
Změněno: 13/3/2019 13:07, Mgr. Pavla Foltynová, Ph.D.

Abstract

V originále

Atomic force microscopy (AFM) helps to describe and explain the mechanobiological properties of living cells on the nanoscale level under physiological conditions. The stiffness of cells is an important parameter reflecting cell physiology. Here, we have provided the first study of the stiffness of cryopreserved cells during post- thawing regeneration using AFM combined with confocal fluorescence microscopy. We demonstrated that the nonfrozen cell stiffness decreased proportionally to the cryoprotectant concentration in the medium. AFM allowed us to map cell surface reconstitution in real time after a freeze/thaw cycle and to monitor the regeneration processes at different depths of the cell and even different parts of the cell surface (nucleus and edge). Fluorescence microscopy showed that the cytoskeleton in fibroblasts, though damaged by the freeze/thaw cycle, is reconstructed after long-termplating. Confocalmicroscopy confirmed that structural changes affect the nuclear envelopes in cryopreserved cells. AFM nanoindentation analysis could be used as a noninvasive method to identify cells that have regenerated their surface mechanical properties with the proper dynamics and to a sufficient degree. This identification can be important particularly in the field of in vitro fertilization and in future cell-based regeneration strategies.

Links

GBP302/12/G157, research and development project
Name: Dynamika a organizace chromosomů během buněčného cyklu a při diferenciaci v normě a patologii
Investor: Czech Science Foundation
LM2015043, research and development project
Name: Česká infrastruktura pro integrativní strukturní biologii (Acronym: CIISB)
Investor: Ministry of Education, Youth and Sports of the CR
LQ1601, research and development project
Name: CEITEC 2020 (Acronym: CEITEC2020)
Investor: Ministry of Education, Youth and Sports of the CR