LACINA, Karel, Jakub SOPOUŠEK, Veronika ČUNDERLOVÁ, Antonín HLAVÁČEK, Tomáš VÁCLAVEK and Veronika LACINOVÁ. Biosensing based on electrochemical impedance spectroscopy: Influence of the often-ignored molecular charge. ELECTROCHEMISTRY COMMUNICATIONS. NEW YORK: ELSEVIER SCIENCE INC, 2018, vol. 93, AUG, p. 183-186. ISSN 1388-2481. doi:10.1016/j.elecom.2018.07.015.
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Basic information
Original name Biosensing based on electrochemical impedance spectroscopy: Influence of the often-ignored molecular charge
Authors LACINA, Karel (203 Czech Republic, guarantor, belonging to the institution), Jakub SOPOUŠEK (203 Czech Republic, belonging to the institution), Veronika ČUNDERLOVÁ (203 Czech Republic, belonging to the institution), Antonín HLAVÁČEK (203 Czech Republic), Tomáš VÁCLAVEK (203 Czech Republic, belonging to the institution) and Veronika LACINOVÁ (203 Czech Republic).
Other information
Original language English
Type of outcome Article in a journal
Field of Study 10406 Analytical chemistry
Country of publisher United States of America
Confidentiality degree is not subject to a state or trade secret
Impact factor Impact factor: 4.197
RIV identification code RIV/00216224:14740/18:00101538
Organization unit Central European Institute of Technology
UT WoS 000439858800040
Keywords in English Biosensing; Immunosensing; Electrochemical impedance spectroscopy; Proteins; Positive charge
Tags rivok
Tags International impact, Reviewed
Changed by Changed by: Mgr. Pavla Foltynová, Ph.D., učo 106624. Changed: 13/3/2019 12:54.
When measurement of electrochemical impedance is used as an analytical method for affinity biosensors, its increase is in the most cases considered as a proof of specific binding. The increase of the mass in the protein layer is expected to be the cause. However, we encountered severe inconsistencies for these assumptions. In our experiments during the development of an immunosensor, proteins with diverse charges were immobilised and captured on the electrode surface. The measured values of impedance were predominantly affected by the charge of these proteins - negatively charged proteins caused (an expected) increase of the impedance values. Contrary, immobilization/capture of positively charged proteins and other compounds caused (a surprising) relative decrease of the impedance. The obvious and logical origin of this behaviour - a repulsive or attractive ionic interactions between protein layers and negatively charged redox probe [Fe(CN)(6)](4-/3-) (commonly used in electrochemistry) - is usually neglected throughout the literature although some fragmental information could be traced. Besides, we want to emphasize here the importance of the additional characterization of the studied system using for example gel electrophoresis, since many unexpected and overlooked factors can influence the electrochemical response upon analyte binding.
GBP206/12/G014, research and development projectName: Centrum pokročilých bioanalytických technologií
LQ1601, research and development projectName: CEITEC 2020 (Acronym: CEITEC2020)
Investor: Ministry of Education, Youth and Sports of the CR
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