| JURÁČEK, Jaroslav, Barbora PELTANOVÁ, Jan DOLEŽEL, Michal FEDORKO, Dalibor PACÍK, Lenka RADOVÁ, Petra VESELA, Marek SVOBODA, Ondřej SLABÝ and Michal STANÍK. Genome-wide identification of urinary cell-free microRNAs for non-invasive detection of bladder cancer. Journal of Cellular and Molecular Medicine. Hoboken, NJ USA: Wiley, 2018, vol. 22, No 3, p. 2033-2038. ISSN 1582-4934. Available from: https://dx.doi.org/10.1111/jcmm.13487. |
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@article{1485296,
author = {Juráček, Jaroslav and Peltanová, Barbora and Doležel, Jan and Fedorko, Michal and Pacík, Dalibor and Radová, Lenka and Vesela, Petra and Svoboda, Marek and Slabý, Ondřej and Staník, Michal},
article_location = {Hoboken, NJ USA},
article_number = {3},
doi = {http://dx.doi.org/10.1111/jcmm.13487},
keywords = {bladder cancer; cell-free miRNAs; urine; biomarker; non-invasive diagnosis},
language = {eng},
issn = {1582-4934},
journal = {Journal of Cellular and Molecular Medicine},
title = {Genome-wide identification of urinary cell-free microRNAs for non-invasive detection of bladder cancer},
volume = {22},
year = {2018}
}
TY - JOUR
ID - 1485296
AU - Juráček, Jaroslav - Peltanová, Barbora - Doležel, Jan - Fedorko, Michal - Pacík, Dalibor - Radová, Lenka - Vesela, Petra - Svoboda, Marek - Slabý, Ondřej - Staník, Michal
PY - 2018
TI - Genome-wide identification of urinary cell-free microRNAs for non-invasive detection of bladder cancer
JF - Journal of Cellular and Molecular Medicine
VL - 22
IS - 3
SP - 2033-2038
EP - 2033-2038
PB - Wiley
SN - 15824934
KW - bladder cancer
KW - cell-free miRNAs
KW - urine
KW - biomarker
KW - non-invasive diagnosis
N2 - Urinary microRNAs (miRNAs) are emerging as clinically useful tool for early and non-invasive detection of various types of cancer including bladder cancer (BCA). In this study, 205 patients with BCA and 99 healthy controls were prospectively enrolled. Expression profiles of urinary miRNAs were obtained using Affymetrix miRNA microarrays (2578 miRNAs) and candidate miRNAs further validated in independent cohorts using qRT-PCR. Whole-genome profiling identified 76 miRNAs with significantly different concentrations in urine of BCA compared to controls (P < 0.01). In the training and independent validation phase of the study, miR-31-5p, miR-93-5p and miR-191-5p were confirmed to have significantly higher levels in urine of patients with BCA in comparison with controls (P < 0.01). We further established 2-miRNA-based urinary DxScore (miR-93-5p, miR-31-5p) enabling sensitive BCA detection with AUC being 0.84 and 0.81 in the training and validation phase, respectively. Moreover, DxScore significantly differed in the various histopathological subgroups of BCA and decreased post-operatively. In conclusion, we identified and independently validated cell-free urinary miRNAs as promising biomarkers enabling non-invasive detection of BCA.
ER -
JURÁČEK, Jaroslav, Barbora PELTANOVÁ, Jan DOLEŽEL, Michal FEDORKO, Dalibor PACÍK, Lenka RADOVÁ, Petra VESELA, Marek SVOBODA, Ondřej SLABÝ and Michal STANÍK. Genome-wide identification of urinary cell-free microRNAs for non-invasive detection of bladder cancer. \textit{Journal of Cellular and Molecular Medicine}. Hoboken, NJ USA: Wiley, 2018, vol.~22, No~3, p.~2033-2038. ISSN~1582-4934. Available from: https://dx.doi.org/10.1111/jcmm.13487.
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