Identification of algal growth inhibitors in treated waste
water using effect-directed analysis based on non-target
screening techniques

J 2018

Identification of algal growth inhibitors in treated waste water using effect-directed analysis based on non-target screening techniques

TOUŠOVÁ, Zuzana, Jean FROMENT, Peter OSWALD, Jaroslav SLOBODNÍK, Klára HILSCHEROVÁ et. al.

Basic information

Original name

Identification of algal growth inhibitors in treated waste water using effect-directed analysis based on non-target screening techniques

Authors

TOUŠOVÁ, Zuzana (203 Czech Republic, belonging to the institution), Jean FROMENT (276 Germany), Peter OSWALD (703 Slovakia), Jaroslav SLOBODNÍK (703 Slovakia), Klára HILSCHEROVÁ (203 Czech Republic, belonging to the institution), Kevin V. THOMAS (36 Australia), Knut Erik TOLLEFSEN (578 Norway), Malcolm REID (578 Norway), Katherine LANGFORD (578 Norway) and Luděk BLÁHA (203 Czech Republic, guarantor, belonging to the institution)

Edition

Journal of Hazardous Materials, Amsterdam, Elsevier Science BV. 2018, 0304-3894

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10511 Environmental sciences

Country of publisher

Netherlands

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

URL

Impact factor

Impact factor: 7.650

RIV identification code

RIV/00216224:14310/18:00105657

Organization unit

Faculty of Science

DOI

http://dx.doi.org/10.1016/j.jhazmat.2018.05.031

UT WoS

000442334400054

Keywords in English

Contaminants of emerging concern; Effect directed analysis; Fractionation; Non-target screening; Toxicity drivers

Abstract

V originále

Growth inhibition of freshwater microalga Pseudokirchneriella subcapitata caused by a waste water treatment plant (WWTP) effluent extract was investigated using an effect directed analysis (EDA) approach. The objective was to identify compounds responsible for the toxicity by combining state-of-the-art sampling, bioanalytical, fractionation and non-target screening techniques. Three fractionation steps of the whole extract were performed and bioactive fractions were analysed with GC (xGC)-MS and LC-HRMS. In total, 383 compounds were tentatively identified, and their toxicity was characterized using US EPA Ecotox database, open scientific literature or modelled by ECOSAR. Among the top-ranking drivers of toxicity were pesticides and their transformation products, pharmaceuticals (barbiturate derivatives and macrolide antibiotics e.g. azithromycin), industrial compounds or caffeine and its metabolites. Several of the top-ranking pesticides are no longer registered for use in plant protection products or biocides in the Czech Republic (e.g. prometryn, atrazine, acetochlor, resmethrin) and some are approved only for use in biocides (e.g. terbutryn, carbendazim, phenothrin), which indicates that their non-agricultural input into aquatic environment via WWT'Ps should be carefully considered. The study demonstrated a functional strategy of combining biotesting, fractionation and non-target screening techniques in the EDA study focused on the identification of algal growth inhibitors in WWTP effluent.

Links

CZ.02.1.01/0.0/0.0/16_013/0001761, interní kód MU

Name: RECETOX RI - OP VVV (Acronym: RECETOX RI)

Investor: Ministry of Education, Youth and Sports of the CR, Priority axis 1: Strengthening capacities for high-quality research

LM2015051, research and development project

Name: Centrum pro výzkum toxických látek v prostředí (Acronym: RECETOX RI)

Investor: Ministry of Education, Youth and Sports of the CR

603437, interní kód MU

Name: SOLUTIONS - Solutions for present and future emerging pollutants in land and water resources management (Acronym: SOLUTIONS)

Investor: European Union, Cooperation

Citovat

TOUŠOVÁ, Zuzana, Jean FROMENT, Peter OSWALD, Jaroslav SLOBODNÍK, Klára HILSCHEROVÁ, Kevin V. THOMAS, Knut Erik TOLLEFSEN, Malcolm REID, Katherine LANGFORD and Luděk BLÁHA. Identification of algal growth inhibitors in treated waste water using effect-directed analysis based on non-target screening techniques. Journal of Hazardous Materials. Amsterdam: Elsevier Science BV., 2018, vol. 358, September, p. 494-502. ISSN 0304-3894. Available from: https://dx.doi.org/10.1016/j.jhazmat.2018.05.031.

@article{1488073,
   author = {Toušová, Zuzana and Froment, Jean and Oswald, Peter and Slobodník, Jaroslav and Hilscherová, Klára and Thomas, Kevin V. and Tollefsen, Knut Erik and Reid, Malcolm and Langford, Katherine and Bláha, Luděk},
   article_location = {Amsterdam},
   article_number = {September},
   doi = {http://dx.doi.org/10.1016/j.jhazmat.2018.05.031},
   keywords = {Contaminants of emerging concern; Effect directed analysis; Fractionation; Non-target screening; Toxicity drivers},
   language = {eng},
   issn = {0304-3894},
   journal = {Journal of Hazardous Materials},
   title = {Identification of algal growth inhibitors in treated waste water using effect-directed analysis based on non-target screening techniques},
   url = {https://www.sciencedirect.com/science/article/pii/S0304389418303807?via%3Dihub},
   volume = {358},
   year = {2018}
}

TY  - JOUR
ID  - 1488073
AU  - Toušová, Zuzana - Froment, Jean - Oswald, Peter - Slobodník, Jaroslav - Hilscherová, Klára - Thomas, Kevin V. - Tollefsen, Knut Erik - Reid, Malcolm - Langford, Katherine - Bláha, Luděk
PY  - 2018
TI  - Identification of algal growth inhibitors in treated waste water using effect-directed analysis based on non-target screening techniques
JF  - Journal of Hazardous Materials
VL  - 358
IS  - September
SP  - 494-502
EP  - 494-502
PB  - Elsevier Science BV.
SN  - 03043894
KW  - Contaminants of emerging concern
KW  - Effect directed analysis
KW  - Fractionation
KW  - Non-target screening
KW  - Toxicity drivers
UR  - https://www.sciencedirect.com/science/article/pii/S0304389418303807?via%3Dihub
L2  - https://www.sciencedirect.com/science/article/pii/S0304389418303807?via%3Dihub
N2  - Growth inhibition of freshwater microalga Pseudokirchneriella subcapitata caused by a waste water treatment plant (WWTP) effluent extract was investigated using an effect directed analysis (EDA) approach. The objective was to identify compounds responsible for the toxicity by combining state-of-the-art sampling, bioanalytical, fractionation and non-target screening techniques. Three fractionation steps of the whole extract were performed and bioactive fractions were analysed with GC (xGC)-MS and LC-HRMS. In total, 383 compounds were tentatively identified, and their toxicity was characterized using US EPA Ecotox database, open scientific literature or modelled by ECOSAR. Among the top-ranking drivers of toxicity were pesticides and their transformation products, pharmaceuticals (barbiturate derivatives and macrolide antibiotics e.g. azithromycin), industrial compounds or caffeine and its metabolites. Several of the top-ranking pesticides are no longer registered for use in plant protection products or biocides in the Czech Republic (e.g. prometryn, atrazine, acetochlor, resmethrin) and some are approved only for use in biocides (e.g. terbutryn, carbendazim, phenothrin), which indicates that their non-agricultural input into aquatic environment via WWT'Ps should be carefully considered. The study demonstrated a functional strategy of combining biotesting, fractionation and non-target screening techniques in the EDA study focused on the identification of algal growth inhibitors in WWTP effluent.
ER  -

TOUŠOVÁ, Zuzana, Jean FROMENT, Peter OSWALD, Jaroslav SLOBODNÍK, Klára HILSCHEROVÁ, Kevin V. THOMAS, Knut Erik TOLLEFSEN, Malcolm REID, Katherine LANGFORD and Luděk BLÁHA. Identification of algal growth inhibitors in treated waste water using effect-directed analysis based on non-target screening techniques. \textit{Journal of Hazardous Materials}. Amsterdam: Elsevier Science BV., 2018, vol.~358, September, p.~494-502. ISSN~0304-3894. Available from: https://dx.doi.org/10.1016/j.jhazmat.2018.05.031.

Detailed Information on Publication Record