The first next-generation sequencing approach to the mitochondrial phylogeny of African monogenean parasites (Platyhelminthes: Gyrodactylidae and Dactylogyridae)

VANHOVE, Maarten Pieterjan, Andy G BRISCOE, Michiel WP JORISSEN, D Tim J LITTLEWOOD and Tine HUYSE. The first next-generation sequencing approach to the mitochondrial phylogeny of African monogenean parasites (Platyhelminthes: Gyrodactylidae and Dactylogyridae). BMC Genomics. LONDON: BioMed Central, 2018, vol. 19, No 520, p. 1-16. ISSN 1471-2164. Available from: https://dx.doi.org/10.1186/s12864-018-4893-5.

Basic information

• Original name: The first next-generation sequencing approach to the mitochondrial phylogeny of African monogenean parasites (Platyhelminthes: Gyrodactylidae and Dactylogyridae)
• Authors: VANHOVE, Maarten Pieterjan (56 Belgium, guarantor, belonging to the institution), Andy G BRISCOE (826 United Kingdom of Great Britain and Northern Ireland), Michiel WP JORISSEN (56 Belgium), D Tim J LITTLEWOOD (826 United Kingdom of Great Britain and Northern Ireland) and Tine HUYSE (56 Belgium).
• Edition: BMC Genomics, LONDON, BioMed Central, 2018, 1471-2164.

Other information

• Original language: English
• Type of outcome: Article in a journal
• Field of Study: 10613 Zoology
• Country of publisher: United Kingdom of Great Britain and Northern Ireland
• Confidentiality degree: is not subject to a state or trade secret
• WWW: URL
• Impact factor: Impact factor: 3.501
• RIV identification code: RIV/00216224:14310/18:00101610
• Organization unit: Faculty of Science
• Doi: http://dx.doi.org/10.1186/s12864-018-4893-5
• UT WoS: 000437515000003
• Keywords in English: Cichlidae; Clariidae; Cichlidogyrus; Gene order; Gyrodactylus; Macrogyrodactylus; Mitogenome; Monogenea; Monopisthocotylea; Phylogenomics

Abstract

Background: Monogenean flatworms are the main ectoparasites of fishes. Representatives of the species-rich families Gyrodactylidae and Dactylogyridae, especially those infecting cichlid fishes and clariid catfishes, are important parasites in African aquaculture, even more so due to the massive anthropogenic translocation of their hosts worldwide. Several questions on their evolution, such as the phylogenetic position of Macrogyrodactylus and the highly speciose Gyrodactylus, remain unresolved with available molecular markers. Also, diagnostics and population-level research would benefit from the development of higher-resolution genetic markers. We aim to offer genetic resources for work on African monogeneans by providing mitogenomic data of four species (two belonging to Gyrodactylidae, two to Dactylogyridae), and analysing their gene sequences and gene order from a phylogenetic perspective. Results: Using Illumina technology, the first four mitochondrial genomes of African monogeneans were assembled and annotated for the cichlid parasites Gyrodactylus nyanzae, Cichlidogyrus haN, Cichlidogyrus mbirizei (near-complete mitogenome) and the catfish parasite Macrogyrodactylus karibae (near-complete mitogenome). Complete nuclear ribosomal operons were also retrieved, as molecular vouchers. The start codon TTG is new for Gyrodactylus and for Dactylogyridae, as is the incomplete stop codon TA for Dactylogyridae. Especially the nad2 gene is promising for primer development. Gene order was identical for protein-coding genes and differed between the African representatives of these families only in a tRNA gene transposition. A mitochondrial phylogeny based on an alignment of nearly 12,500 bp including 12 protein-coding and two ribosomal RNA genes confirms that the Neotropical oviparous Aglaiogyrodactylus forficulatus takes a sister group position with respect to the other gyrodactylids, instead of the supposedly 'primitive' African Macrogyrodactylus. Inclusion of the African Gyrodactylus nyanzae confirms the paraphyly of Gyrodactylus. The position of the African dactylogyrid Cichlidogyrus is unresolved, although gene order suggests it is closely related to marine ancyrocephalines. Conclusions: The amount of mitogenomic data available for gyrodactylids and dactylogyrids is increased by roughly one-third. Our study underscores the potential of mitochondrial genes and gene order in flatworm phylogenetics, and of next-generation sequencing for marker development for these non-model helminths for which few primers are available.

Links

• GBP505/12/G112, research and development project: Name: ECIP - Evropské centrum ichtyoparazitologie

Investor: Czech Science Foundation