The first next-generation sequencing approach to the
mitochondrial phylogeny of African monogenean parasites
(Platyhelminthes: Gyrodactylidae and Dactylogyridae)

J 2018

The first next-generation sequencing approach to the mitochondrial phylogeny of African monogenean parasites (Platyhelminthes: Gyrodactylidae and Dactylogyridae)

VANHOVE, Maarten Pieterjan, Andy G BRISCOE, Michiel WP JORISSEN, D Tim J LITTLEWOOD, Tine HUYSE et. al.

Basic information

Original name

The first next-generation sequencing approach to the mitochondrial phylogeny of African monogenean parasites (Platyhelminthes: Gyrodactylidae and Dactylogyridae)

Authors

VANHOVE, Maarten Pieterjan (56 Belgium, guarantor, belonging to the institution), Andy G BRISCOE (826 United Kingdom of Great Britain and Northern Ireland), Michiel WP JORISSEN (56 Belgium), D Tim J LITTLEWOOD (826 United Kingdom of Great Britain and Northern Ireland) and Tine HUYSE (56 Belgium)

Edition

BMC Genomics, LONDON, BioMed Central, 2018, 1471-2164

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10613 Zoology

Country of publisher

United Kingdom of Great Britain and Northern Ireland

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

URL

Impact factor

Impact factor: 3.501

RIV identification code

RIV/00216224:14310/18:00101610

Organization unit

Faculty of Science

DOI

http://dx.doi.org/10.1186/s12864-018-4893-5

UT WoS

000437515000003

Keywords in English

Cichlidae; Clariidae; Cichlidogyrus; Gene order; Gyrodactylus; Macrogyrodactylus; Mitogenome; Monogenea; Monopisthocotylea; Phylogenomics

Změněno: 23/4/2024 13:25, Mgr. Michal Petr

Abstract

V originále

Background: Monogenean flatworms are the main ectoparasites of fishes. Representatives of the species-rich families Gyrodactylidae and Dactylogyridae, especially those infecting cichlid fishes and clariid catfishes, are important parasites in African aquaculture, even more so due to the massive anthropogenic translocation of their hosts worldwide. Several questions on their evolution, such as the phylogenetic position of Macrogyrodactylus and the highly speciose Gyrodactylus, remain unresolved with available molecular markers. Also, diagnostics and population-level research would benefit from the development of higher-resolution genetic markers. We aim to offer genetic resources for work on African monogeneans by providing mitogenomic data of four species (two belonging to Gyrodactylidae, two to Dactylogyridae), and analysing their gene sequences and gene order from a phylogenetic perspective. Results: Using Illumina technology, the first four mitochondrial genomes of African monogeneans were assembled and annotated for the cichlid parasites Gyrodactylus nyanzae, Cichlidogyrus haN, Cichlidogyrus mbirizei (near-complete mitogenome) and the catfish parasite Macrogyrodactylus karibae (near-complete mitogenome). Complete nuclear ribosomal operons were also retrieved, as molecular vouchers. The start codon TTG is new for Gyrodactylus and for Dactylogyridae, as is the incomplete stop codon TA for Dactylogyridae. Especially the nad2 gene is promising for primer development. Gene order was identical for protein-coding genes and differed between the African representatives of these families only in a tRNA gene transposition. A mitochondrial phylogeny based on an alignment of nearly 12,500 bp including 12 protein-coding and two ribosomal RNA genes confirms that the Neotropical oviparous Aglaiogyrodactylus forficulatus takes a sister group position with respect to the other gyrodactylids, instead of the supposedly 'primitive' African Macrogyrodactylus. Inclusion of the African Gyrodactylus nyanzae confirms the paraphyly of Gyrodactylus. The position of the African dactylogyrid Cichlidogyrus is unresolved, although gene order suggests it is closely related to marine ancyrocephalines. Conclusions: The amount of mitogenomic data available for gyrodactylids and dactylogyrids is increased by roughly one-third. Our study underscores the potential of mitochondrial genes and gene order in flatworm phylogenetics, and of next-generation sequencing for marker development for these non-model helminths for which few primers are available.

Links

GBP505/12/G112, research and development project

Name: ECIP - Evropské centrum ichtyoparazitologie

Investor: Czech Science Foundation

Citovat

VANHOVE, Maarten Pieterjan, Andy G BRISCOE, Michiel WP JORISSEN, D Tim J LITTLEWOOD and Tine HUYSE. The first next-generation sequencing approach to the mitochondrial phylogeny of African monogenean parasites (Platyhelminthes: Gyrodactylidae and Dactylogyridae). BMC Genomics. LONDON: BioMed Central, 2018, vol. 19, No 520, p. 1-16. ISSN 1471-2164. Available from: https://dx.doi.org/10.1186/s12864-018-4893-5.

@article{1488420,
   author = {Vanhove, Maarten Pieterjan and Briscoe, Andy G and Jorissen, Michiel WP and Littlewood, D Tim J and Huyse, Tine},
   article_location = {LONDON},
   article_number = {520},
   doi = {http://dx.doi.org/10.1186/s12864-018-4893-5},
   keywords = {Cichlidae; Clariidae; Cichlidogyrus; Gene order; Gyrodactylus; Macrogyrodactylus; Mitogenome; Monogenea; Monopisthocotylea; Phylogenomics},
   language = {eng},
   issn = {1471-2164},
   journal = {BMC Genomics},
   title = {The first next-generation sequencing approach to the mitochondrial phylogeny of African monogenean parasites (Platyhelminthes: Gyrodactylidae and Dactylogyridae)},
   url = {http://dx.doi.org/10.1186/s12864-018-4893-5},
   volume = {19},
   year = {2018}
}

TY  - JOUR
ID  - 1488420
AU  - Vanhove, Maarten Pieterjan - Briscoe, Andy G - Jorissen, Michiel WP - Littlewood, D Tim J - Huyse, Tine
PY  - 2018
TI  - The first next-generation sequencing approach to the mitochondrial phylogeny of African monogenean parasites (Platyhelminthes: Gyrodactylidae and Dactylogyridae)
JF  - BMC Genomics
VL  - 19
IS  - 520
SP  - 1-16
EP  - 1-16
PB  - BioMed Central
SN  - 14712164
KW  - Cichlidae
KW  - Clariidae
KW  - Cichlidogyrus
KW  - Gene order
KW  - Gyrodactylus
KW  - Macrogyrodactylus
KW  - Mitogenome
KW  - Monogenea
KW  - Monopisthocotylea
KW  - Phylogenomics
UR  - http://dx.doi.org/10.1186/s12864-018-4893-5
N2  - Background: Monogenean flatworms are the main ectoparasites of fishes. Representatives of the species-rich families Gyrodactylidae and Dactylogyridae, especially those infecting cichlid fishes and clariid catfishes, are important parasites in African aquaculture, even more so due to the massive anthropogenic translocation of their hosts worldwide. Several questions on their evolution, such as the phylogenetic position of Macrogyrodactylus and the highly speciose Gyrodactylus, remain unresolved with available molecular markers. Also, diagnostics and population-level research would benefit from the development of higher-resolution genetic markers. We aim to offer genetic resources for work on African monogeneans by providing mitogenomic data of four species (two belonging to Gyrodactylidae, two to Dactylogyridae), and analysing their gene sequences and gene order from a phylogenetic perspective. Results: Using Illumina technology, the first four mitochondrial genomes of African monogeneans were assembled and annotated for the cichlid parasites Gyrodactylus nyanzae, Cichlidogyrus haN, Cichlidogyrus mbirizei (near-complete mitogenome) and the catfish parasite Macrogyrodactylus karibae (near-complete mitogenome). Complete nuclear ribosomal operons were also retrieved, as molecular vouchers. The start codon TTG is new for Gyrodactylus and for Dactylogyridae, as is the incomplete stop codon TA for Dactylogyridae. Especially the nad2 gene is promising for primer development. Gene order was identical for protein-coding genes and differed between the African representatives of these families only in a tRNA gene transposition. A mitochondrial phylogeny based on an alignment of nearly 12,500 bp including 12 protein-coding and two ribosomal RNA genes confirms that the Neotropical oviparous Aglaiogyrodactylus forficulatus takes a sister group position with respect to the other gyrodactylids, instead of the supposedly 'primitive' African Macrogyrodactylus. Inclusion of the African Gyrodactylus nyanzae confirms the paraphyly of Gyrodactylus. The position of the African dactylogyrid Cichlidogyrus is unresolved, although gene order suggests it is closely related to marine ancyrocephalines. Conclusions: The amount of mitogenomic data available for gyrodactylids and dactylogyrids is increased by roughly one-third. Our study underscores the potential of mitochondrial genes and gene order in flatworm phylogenetics, and of next-generation sequencing for marker development for these non-model helminths for which few primers are available.
ER  -

VANHOVE, Maarten Pieterjan, Andy G BRISCOE, Michiel WP JORISSEN, D Tim J LITTLEWOOD and Tine HUYSE. The first next-generation sequencing approach to the mitochondrial phylogeny of African monogenean parasites (Platyhelminthes: Gyrodactylidae and Dactylogyridae). \textit{BMC Genomics}. LONDON: BioMed Central, 2018, vol.~19, No~520, p.~1-16. ISSN~1471-2164. Available from: https://dx.doi.org/10.1186/s12864-018-4893-5.

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