Detailed Information on Publication Record
2018
Circulating PIWI-Interacting RNAs piR-5937 and piR-28876 Are Promising Diagnostic Biomarkers of Colon Cancer
VYCHYTILOVÁ, Petra, Karolína ŠTÍTKOVCOVÁ, Lenka RADOVÁ, Milana ŠACHLOVÁ, Zdeňka HERUDKOVÁ et. al.Basic information
Original name
Circulating PIWI-Interacting RNAs piR-5937 and piR-28876 Are Promising Diagnostic Biomarkers of Colon Cancer
Authors
VYCHYTILOVÁ, Petra (203 Czech Republic, belonging to the institution), Karolína ŠTÍTKOVCOVÁ (203 Czech Republic, belonging to the institution), Lenka RADOVÁ (203 Czech Republic, belonging to the institution), Milana ŠACHLOVÁ (203 Czech Republic, belonging to the institution), Zdeňka HERUDKOVÁ (203 Czech Republic, belonging to the institution), Kateřina SLABÁ (203 Czech Republic), Zdeněk KALA (203 Czech Republic, belonging to the institution), Marek SVOBODA (203 Czech Republic, belonging to the institution), Igor KISS (203 Czech Republic, belonging to the institution), Rostislav VYZULA (203 Czech Republic, belonging to the institution), William C. CHO (156 China) and Ondřej SLABÝ (203 Czech Republic, guarantor, belonging to the institution)
Edition
CANCER EPIDEMIOLOGY BIOMARKERS & PREVENTION, PHILADELPHIA, AMER ASSOC CANCER RESEARCH, 2018, 1055-9965
Other information
Language
English
Type of outcome
Článek v odborném periodiku
Field of Study
30204 Oncology
Country of publisher
United States of America
Confidentiality degree
není předmětem státního či obchodního tajemství
Impact factor
Impact factor: 5.057
RIV identification code
RIV/00216224:14740/18:00105816
Organization unit
Central European Institute of Technology
UT WoS
000443754900004
Keywords in English
Circulating PIWI-interacting RNAs
Tags
International impact, Reviewed
Změněno: 13/3/2019 13:06, Mgr. Pavla Foltynová, Ph.D.
Abstract
V originále
Background: The early detection of colon cancer is one of the main prerequisites for successful treatment and mortality reduction. Circulating PIWI-interacting RNAs (piRNA) were recently identified as novel promising biomarkers. The purpose of the study was to assess the profiles of piRNAs in blood serum of colon cancer patients with the aim to identify those with high diagnostic potential. Methods: Blood serum samples from 403 colon cancer patients and 276 healthy donors were included in this 3-phase biomarker study. Large-scale piRNA expression profiling was performed using Illumina small RNA sequencing. The diagnostic potential of selected piRNAs was further validated on independent training and validation sets of samples using RT-qPCR. Results: In total, 31 piRNAs were found to be significantly deregulated in serum of cancer patients compared with healthy donors. Based on the levels of piR-5937 and piR-28876, it was possible to differentiate between cancer patients and healthy donors with high sensitivity and specificity. Moreover, both piRNAs exhibited satisfactory diagnostic performance also in patients with stage I disease and enabled detection of colon cancer with higher sensitivity than currently used biomarkers CEA and CA19-9. Finally, the expression of analyzed piRNAs in blood restored significantly 1 month after the surgical resection. Conclusions: Based on our findings, piRNAs are abundant in human blood serum. Furthermore, their levels in colon cancer have been observed to be significantly deregulated. However, their involvement in carcinogenesis must be further established. Impact: piRNAs could serve as promising noninvasive biomarkers for early detection of colon cancer. (C) 2018 AACR.
Links
| LQ1601, research and development project |
| ||
| NV16-31765A, research and development project |
|