J 2018

Extensive Genetic Commonality among Wildlife, Wastewater, Community, and Nosocomial Isolates of Escherichia coli Sequence Type 131 (H30R1 and H30Rx Subclones) That Carry bla(CTX-M-27) or bla(CTX-M-15)

JAMBOROVA, Ivana, Brian D. JOHNSTON, Ivo PAPOUSEK, Katerina KACHLIKOVA, Lenka MICENKOVÁ et. al.

Basic information

Original name

Extensive Genetic Commonality among Wildlife, Wastewater, Community, and Nosocomial Isolates of Escherichia coli Sequence Type 131 (H30R1 and H30Rx Subclones) That Carry bla(CTX-M-27) or bla(CTX-M-15)

Authors

JAMBOROVA, Ivana (203 Czech Republic, guarantor), Brian D. JOHNSTON (840 United States of America), Ivo PAPOUSEK (203 Czech Republic), Katerina KACHLIKOVA (203 Czech Republic), Lenka MICENKOVÁ (703 Slovakia, belonging to the institution), Connie CLABOTS (840 United States of America), Anna SKALOVA (203 Czech Republic), Katerina CHUDEJOVA (203 Czech Republic), Monika DOLEJSKA (203 Czech Republic), Ivan LITERAK (203 Czech Republic) and James R. JOHNSON (203 Czech Republic)

Edition

Antimicrobial Agents and Chemotherapy, Washington, American Society for Microbiology, 2018, 0066-4804

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10606 Microbiology

Country of publisher

United States of America

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

Impact factor

Impact factor: 4.715

RIV identification code

RIV/00216224:14310/18:00105836

Organization unit

Faculty of Science

DOI

UT WoS

000445405500011

Keywords in English

Escherichia coli ST131; ESBL; virulence; nosocomial and community-acquired infections; wildlife; environment

Tags

Tags

International impact, Reviewed
Změněno: 23/4/2024 14:07, Mgr. Michal Petr

Abstract

V originále

Escherichia coli sequence type 131 (ST131) is currently one of the leading causes of multidrug-resistant extraintestinal infections globally. Here, we analyzed the phenotypic and genotypic characteristics of 169 ST131 isolates from various sources (wildlife, wastewater, companion animals, community, and hospitals) to determine whether wildlife and the environment share similar strains with humans, supporting transmission of ST131 between different ecological niches. Susceptibility to 32 antimicro-bials was tested by disc diffusion and broth microdilution. Antibiotic resistance genes, integrons, plasmid replicons, 52 virulence genes, and fimH-based subtypes were detected by PCR and DNA sequencing. Genomic relatedness was determined by pulsed-field gel electrophoresis (PFGE). The genetic context and plasmid versus chromosomal location of extended-spectrum beta-lactamase and AmpC beta-lactamase genes was determined by PCR and probe hybridization, respectively. The 169 ST131 study isolates segregated predominantly into bla(CTX-M-15) H30Rx (60%) and bla(CTX-M-27) H30R1 (25%) subclones. Within each subclone, isolates from different source groups were categorized into distinct PFGE clusters; genotypic characteristics were fairly well conserved within each major PFGE cluster. Irrespective of source, the bla(CTX-M-15) H30Rx isolates typically exhibited virotype A (89%), an F2:A1:B- replicon (84%), and a 1.7-kb class 1 integron (92%) and had diverse structures upstream of the bla(CTX-M) region. In contrast, the bla(CTX-M-27) H30R1 isolates typically exhibited virotype C (86%), an F1:A2:B20 replicon (76%), and a conserved IS26-Delta ISEcp1-bla(CTX-M)-like structure. Despite considerable overall genetic diversity, our data demonstrate significant commonality between E. coli ST131 isolates from diverse environments, supporting transmission between different sources, including humans, environment, and wildlife.

Links

EF15_003/0000469, research and development project
Name: Cetocoen Plus
LQ1601, research and development project
Name: CEITEC 2020 (Acronym: CEITEC2020)
Investor: Ministry of Education, Youth and Sports of the CR