Identification of microRNAs differentially expressed in
glioblastoma stem-like cells and their association with patient
survival

J 2018

Identification of microRNAs differentially expressed in glioblastoma stem-like cells and their association with patient survival

ŠÁNA, Jiří, Petr BUŠEK, Pavel FADRUS, Andrej BEŠŠE, Lenka RADOVÁ et. al.

Basic information

Original name

Identification of microRNAs differentially expressed in glioblastoma stem-like cells and their association with patient survival

Authors

ŠÁNA, Jiří (203 Czech Republic, belonging to the institution), Petr BUŠEK (203 Czech Republic), Pavel FADRUS (203 Czech Republic, belonging to the institution), Andrej BEŠŠE (703 Slovakia, belonging to the institution), Lenka RADOVÁ (203 Czech Republic, belonging to the institution), Marek VEČEŘA (703 Slovakia, belonging to the institution), Stefan REGULI (203 Czech Republic), Lucie SROMOVA STOLLINOVA (203 Czech Republic), Marek HILSER (203 Czech Republic), Radim LIPINA (203 Czech Republic, belonging to the institution), Radek LAKOMÝ (203 Czech Republic, belonging to the institution), Leoš KŘEN (203 Czech Republic, belonging to the institution), Martin SMRČKA (203 Czech Republic, belonging to the institution), Aleksi ŠEDO (203 Czech Republic) and Ondřej SLABÝ (203 Czech Republic, guarantor, belonging to the institution)

Edition

Scientific reports, LONDON, NATURE PUBLISHING GROUP, 2018, 2045-2322

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

30204 Oncology

Country of publisher

United Kingdom of Great Britain and Northern Ireland

Confidentiality degree

není předmětem státního či obchodního tajemství

Impact factor

Impact factor: 4.011

RIV identification code

RIV/00216224:14740/18:00105841

Organization unit

Central European Institute of Technology

DOI

http://dx.doi.org/10.1038/s41598-018-20929-6

UT WoS

000424743500052

Keywords in English

Glioblastoma stem-like cells

Abstract

V originále

Glioblastoma stem-like cells (GSCs) are critical for the aggressiveness and progression of glioblastoma (GBM) and contribute to its resistance to adjuvant treatment. MicroRNAs (miRNAs) are small, non-coding RNAs controlling gene expression at the post-transcriptional level, which are known to be important regulators of the stem-like features. Moreover, miRNAs have been previously proved to be promising diagnostic biomarkers in several cancers including GBM. Using global expression analysis of miRNAs in 10 paired in-vitro as well as in-vivo characterized primary GSC and non-stem glioblastoma cultures, we identified a miRNA signature associated with the stem-like phenotype in GBM. 51 most deregulated miRNAs classified the cell cultures into GSC and non-stem cell clusters and identified a subgroup of GSC cultures with more pronounced stem-cell characteristics. The importance of the identified miRNA signature was further supported by demonstrating that a Risk Score based on the expression of seven miRNAs overexpressed in GSC predicted overall survival in GBM patients in the TCGA dataset independently of the IDH1 status. In summary, we identified miRNAs differentially expressed in GSCs and described their association with GBM patient survival. We propose that these miRNAs participate on GSC features and could represent helpful prognostic markers and potential therapeutic targets in GBM.

Links

LM2015064, research and development project

Name: Český národní uzel Evropské infrastruktury pro translační medicínu (Acronym: EATRIS-ERIC-CZ)

Investor: Ministry of Education, Youth and Sports of the CR

LQ1601, research and development project

Name: CEITEC 2020 (Acronym: CEITEC2020)

Investor: Ministry of Education, Youth and Sports of the CR

NV15-33158A, research and development project

Name: Nová úroveň molekulární taxonomie glioblastomu založená na expresních profilech dlouhých nekódujících RNA: implikace pro diagnostiku a terapii

NV15-34553A, research and development project

Name: Využití mikroRNA v mozkomíšním moku pro diagnostiku mozkových nádorů

Citovat

ŠÁNA, Jiří, Petr BUŠEK, Pavel FADRUS, Andrej BEŠŠE, Lenka RADOVÁ, Marek VEČEŘA, Stefan REGULI, Lucie SROMOVA STOLLINOVA, Marek HILSER, Radim LIPINA, Radek LAKOMÝ, Leoš KŘEN, Martin SMRČKA, Aleksi ŠEDO and Ondřej SLABÝ. Identification of microRNAs differentially expressed in glioblastoma stem-like cells and their association with patient survival. Scientific reports. LONDON: NATURE PUBLISHING GROUP, 2018, vol. 8, FEB, p. 2836-2846. ISSN 2045-2322. Available from: https://dx.doi.org/10.1038/s41598-018-20929-6.

@article{1489878,
   author = {Šána, Jiří and Bušek, Petr and Fadrus, Pavel and Bešše, Andrej and Radová, Lenka and Večeřa, Marek and Reguli, Stefan and Sromova Stollinova, Lucie and Hilser, Marek and Lipina, Radim and Lakomý, Radek and Křen, Leoš and Smrčka, Martin and Šedo, Aleksi and Slabý, Ondřej},
   article_location = {LONDON},
   article_number = {FEB},
   doi = {http://dx.doi.org/10.1038/s41598-018-20929-6},
   keywords = {Glioblastoma stem-like cells},
   language = {eng},
   issn = {2045-2322},
   journal = {Scientific reports},
   title = {Identification of microRNAs differentially expressed in glioblastoma stem-like cells and their association with patient survival},
   volume = {8},
   year = {2018}
}

TY  - JOUR
ID  - 1489878
AU  - Šána, Jiří - Bušek, Petr - Fadrus, Pavel - Bešše, Andrej - Radová, Lenka - Večeřa, Marek - Reguli, Stefan - Sromova Stollinova, Lucie - Hilser, Marek - Lipina, Radim - Lakomý, Radek - Křen, Leoš - Smrčka, Martin - Šedo, Aleksi - Slabý, Ondřej
PY  - 2018
TI  - Identification of microRNAs differentially expressed in glioblastoma stem-like cells and their association with patient survival
JF  - Scientific reports
VL  - 8
IS  - FEB
SP  - 2836
EP  - 2836
PB  - NATURE PUBLISHING GROUP
SN  - 20452322
KW  - Glioblastoma stem-like cells
N2  - Glioblastoma stem-like cells (GSCs) are critical for the aggressiveness and progression of glioblastoma (GBM) and contribute to its resistance to adjuvant treatment. MicroRNAs (miRNAs) are small, non-coding RNAs controlling gene expression at the post-transcriptional level, which are known to be important regulators of the stem-like features. Moreover, miRNAs have been previously proved to be promising diagnostic biomarkers in several cancers including GBM. Using global expression analysis of miRNAs in 10 paired in-vitro as well as in-vivo characterized primary GSC and non-stem glioblastoma cultures, we identified a miRNA signature associated with the stem-like phenotype in GBM. 51 most deregulated miRNAs classified the cell cultures into GSC and non-stem cell clusters and identified a subgroup of GSC cultures with more pronounced stem-cell characteristics. The importance of the identified miRNA signature was further supported by demonstrating that a Risk Score based on the expression of seven miRNAs overexpressed in GSC predicted overall survival in GBM patients in the TCGA dataset independently of the IDH1 status. In summary, we identified miRNAs differentially expressed in GSCs and described their association with GBM patient survival. We propose that these miRNAs participate on GSC features and could represent helpful prognostic markers and potential therapeutic targets in GBM.
ER  -

ŠÁNA, Jiří, Petr BUŠEK, Pavel FADRUS, Andrej BEŠŠE, Lenka RADOVÁ, Marek VEČEŘA, Stefan REGULI, Lucie SROMOVA STOLLINOVA, Marek HILSER, Radim LIPINA, Radek LAKOMÝ, Leoš KŘEN, Martin SMRČKA, Aleksi ŠEDO and Ondřej SLABÝ. Identification of microRNAs differentially expressed in glioblastoma stem-like cells and their association with patient survival. \textit{Scientific reports}. LONDON: NATURE PUBLISHING GROUP, 2018, vol.~8, FEB, p.~2836-2846. ISSN~2045-2322. Available from: https://dx.doi.org/10.1038/s41598-018-20929-6.

Detailed Information on Publication Record