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@article{1490096,
author = {Bílková, Zuzana and Kotasová Adámková, Marie and Albrecht, Tomáš and Šimek, Zdeněk},
article_location = {Amsterdam, Netherlands},
article_number = {1590},
doi = {http://dx.doi.org/10.1016/j.chroma.2018.12.069},
keywords = {Feather; Hormones; Birds; Liquid chromatography; Tandem mass spectrometry; Chemical derivatization of ketosteroids},
language = {eng},
issn = {0021-9673},
journal = {Journal of Chromatography A},
title = {Determination of testosterone and corticosterone in feathers using liquid chromatography-mass spectrometry},
url = {https://www.sciencedirect.com/science/article/pii/S0021967318315693?via%3Dihub},
volume = {1590},
year = {2019}
}
TY - JOUR
ID - 1490096
AU - Bílková, Zuzana - Kotasová Adámková, Marie - Albrecht, Tomáš - Šimek, Zdeněk
PY - 2019
TI - Determination of testosterone and corticosterone in feathers using liquid chromatography-mass spectrometry
JF - Journal of Chromatography A
VL - 1590
IS - 1590
SP - 96-103
EP - 96-103
PB - Elsevier
SN - 00219673
KW - Feather
KW - Hormones
KW - Birds
KW - Liquid chromatography
KW - Tandem mass spectrometry
KW - Chemical derivatization of ketosteroids
UR - https://www.sciencedirect.com/science/article/pii/S0021967318315693?via%3Dihub
L2 - https://www.sciencedirect.com/science/article/pii/S0021967318315693?via%3Dihub
N2 - Feathers gradually accumulate hormones and reflect long-term average plasma steroid levels during their growth. Feather hormone levels thus provide for the measurement of plasma hormones concentrations integrated over a period of several days or weeks. In this study, we focused on the development of a method to determine testosterone (TEST) and corticosterone (CORT) levels in extracts from feathers of small bodied birds with a limited amount of feathers available per individual. For this purpose, the method had to be verified for a small weight of samples. The present study describes the effect of the conditions of sample preparation and keto-derivatisation on the sensitivity of the LC-ESI-MS/MS analysis of TEST and CORT. Generally, chemical derivatization improves the sensitivity and selectivity of LC-MS/MS analysis. It can be used particularly in situations when the total amount of collected sample is limited (such as in our studies). Both the conditions of feather sample preparation (the selection of the extraction solvent, the time of extraction, and the conditions of solid phase extraction) and the reaction conditions affecting the formation of keto-derivatives (such as reaction temperature and reaction time) were tested. Methanol as the extraction solvent, 8 h as the extraction time, 50 degrees C as the reaction temperature of derivatization, and 90 min as the reaction time of derivatization are the most suitable conditions in terms of achieving a high sensitivity of analyses. Calibration curves are linear, at least in the range 25-2500 pg mL(-1), which is usually found in feather extracts. The limit of detection (LOD) for TEST and CORT was 1.0 and 0.3 pg per mL, respectively. The limit of quantification (LOQ) for TEST and CORT was 3.3 and 1.0 pg per mL, respectively. The optimized procedure was successfully applied for the analysis of TEST and CORT in real feather samples. The method could be used in a variety of research direction including wildlife, agricultural or veterinary studies.
ER -
BÍLKOVÁ, Zuzana, Marie KOTASOVÁ ADÁMKOVÁ, Tomáš ALBRECHT and Zdeněk ŠIMEK. Determination of testosterone and corticosterone in feathers using liquid chromatography-mass spectrometry. \textit{Journal of Chromatography A}. Amsterdam, Netherlands: Elsevier, 2019, vol.~1590, No~1590, p.~96-103. ISSN~0021-9673. Available from: https://dx.doi.org/10.1016/j.chroma.2018.12.069.
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