MicroRNA isolation and quantification in cerebrospinal fluid: A
comparative methodical study

J 2018

MicroRNA isolation and quantification in cerebrospinal fluid: A comparative methodical study

KOPKOVÁ, Alena, Jiří ŠÁNA, Pavel FADRUS, Táňa MACHÁČKOVÁ, Marek VEČEŘA et. al.

Basic information

Original name

MicroRNA isolation and quantification in cerebrospinal fluid: A comparative methodical study

Authors

KOPKOVÁ, Alena (203 Czech Republic, belonging to the institution), Jiří ŠÁNA (203 Czech Republic, belonging to the institution), Pavel FADRUS (203 Czech Republic, belonging to the institution), Táňa MACHÁČKOVÁ (203 Czech Republic, belonging to the institution), Marek VEČEŘA (703 Slovakia, belonging to the institution), Václav VYBÍHAL (203 Czech Republic, belonging to the institution), Jaroslav JURÁČEK (203 Czech Republic, belonging to the institution), Petra VYCHYTILOVÁ (203 Czech Republic, belonging to the institution), Martin SMRČKA (203 Czech Republic, belonging to the institution) and Ondřej SLABÝ (203 Czech Republic, guarantor, belonging to the institution)

Edition

Plos one, San Francisco, Public Library of Science, 2018, 1932-6203

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

30204 Oncology

Country of publisher

United States of America

Confidentiality degree

není předmětem státního či obchodního tajemství

Impact factor

Impact factor: 2.776

RIV identification code

RIV/00216224:14740/18:00106965

Organization unit

Central European Institute of Technology

DOI

http://dx.doi.org/10.1371/journal.pone.0208580

UT WoS

000452640900023

Keywords in English

MicroRNA; cerebrospinal fluid

Abstract

V originále

Associated with the pathogenesis of many cancers, including brain tumors, microRNAs (miRNAs) present promising diagnostic biomarkers. These molecules have been also studied in cerebrospinal fluid (CSF), showing great potential as a diagnostic tool in patients with brain tumors. Even though there are some biological and technological factors that could affect the results and their biological and clinical interpretation, miRNA analysis in CSF is not fully standardized. This study aims to compare several RNA extraction and miRNA quantification approaches, including high-throughput technologies and individual miRNA detection methods, thereby contributing to the optimization and standardization of quantification of extracellular miRNAs in CSF. Such knowledge is essential for the potential use of miRNAs as diagnostic biomarkers in brain tumors.

Links

NV15-34553A, research and development project

Name: Využití mikroRNA v mozkomíšním moku pro diagnostiku mozkových nádorů

Citovat

KOPKOVÁ, Alena, Jiří ŠÁNA, Pavel FADRUS, Táňa MACHÁČKOVÁ, Marek VEČEŘA, Václav VYBÍHAL, Jaroslav JURÁČEK, Petra VYCHYTILOVÁ, Martin SMRČKA and Ondřej SLABÝ. MicroRNA isolation and quantification in cerebrospinal fluid: A comparative methodical study. Plos one. San Francisco: Public Library of Science, 2018, vol. 13, No 12, p. 1-12. ISSN 1932-6203. Available from: https://dx.doi.org/10.1371/journal.pone.0208580.

@article{1490159,
   author = {Kopková, Alena and Šána, Jiří and Fadrus, Pavel and Macháčková, Táňa and Večeřa, Marek and Vybíhal, Václav and Juráček, Jaroslav and Vychytilová, Petra and Smrčka, Martin and Slabý, Ondřej},
   article_location = {San Francisco},
   article_number = {12},
   doi = {http://dx.doi.org/10.1371/journal.pone.0208580},
   keywords = {MicroRNA; cerebrospinal fluid},
   language = {eng},
   issn = {1932-6203},
   journal = {Plos one},
   title = {MicroRNA isolation and quantification in cerebrospinal fluid: A comparative methodical study},
   volume = {13},
   year = {2018}
}

TY  - JOUR
ID  - 1490159
AU  - Kopková, Alena - Šána, Jiří - Fadrus, Pavel - Macháčková, Táňa - Večeřa, Marek - Vybíhal, Václav - Juráček, Jaroslav - Vychytilová, Petra - Smrčka, Martin - Slabý, Ondřej
PY  - 2018
TI  - MicroRNA isolation and quantification in cerebrospinal fluid: A comparative methodical study
JF  - Plos one
VL  - 13
IS  - 12
SP  - 1-12
EP  - 1-12
PB  - Public Library of Science
SN  - 19326203
KW  - MicroRNA
KW  - cerebrospinal fluid
N2  - Associated with the pathogenesis of many cancers, including brain tumors, microRNAs (miRNAs) present promising diagnostic biomarkers. These molecules have been also studied in cerebrospinal fluid (CSF), showing great potential as a diagnostic tool in patients with brain tumors. Even though there are some biological and technological factors that could affect the results and their biological and clinical interpretation, miRNA analysis in CSF is not fully standardized. This study aims to compare several RNA extraction and miRNA quantification approaches, including high-throughput technologies and individual miRNA detection methods, thereby contributing to the optimization and standardization of quantification of extracellular miRNAs in CSF. Such knowledge is essential for the potential use of miRNAs as diagnostic biomarkers in brain tumors.
ER  -

KOPKOVÁ, Alena, Jiří ŠÁNA, Pavel FADRUS, Táňa MACHÁČKOVÁ, Marek VEČEŘA, Václav VYBÍHAL, Jaroslav JURÁČEK, Petra VYCHYTILOVÁ, Martin SMRČKA and Ondřej SLABÝ. MicroRNA isolation and quantification in cerebrospinal fluid: A comparative methodical study. \textit{Plos one}. San Francisco: Public Library of Science, 2018, vol.~13, No~12, p.~1-12. ISSN~1932-6203. Available from: https://dx.doi.org/10.1371/journal.pone.0208580.

Detailed Information on Publication Record