| KOPKOVÁ, Alena, Jiří ŠÁNA, Pavel FADRUS, Táňa MACHÁČKOVÁ, Marek VEČEŘA, Václav VYBÍHAL, Jaroslav JURÁČEK, Petra VYCHYTILOVÁ, Martin SMRČKA and Ondřej SLABÝ. MicroRNA isolation and quantification in cerebrospinal fluid: A comparative methodical study. Plos one. San Francisco: Public Library of Science, 2018, vol. 13, No 12, p. 1-12. ISSN 1932-6203. Available from: https://dx.doi.org/10.1371/journal.pone.0208580. |
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@article{1490159,
author = {Kopková, Alena and Šána, Jiří and Fadrus, Pavel and Macháčková, Táňa and Večeřa, Marek and Vybíhal, Václav and Juráček, Jaroslav and Vychytilová, Petra and Smrčka, Martin and Slabý, Ondřej},
article_location = {San Francisco},
article_number = {12},
doi = {http://dx.doi.org/10.1371/journal.pone.0208580},
keywords = {MicroRNA; cerebrospinal fluid},
language = {eng},
issn = {1932-6203},
journal = {Plos one},
title = {MicroRNA isolation and quantification in cerebrospinal fluid: A comparative methodical study},
volume = {13},
year = {2018}
}
TY - JOUR
ID - 1490159
AU - Kopková, Alena - Šána, Jiří - Fadrus, Pavel - Macháčková, Táňa - Večeřa, Marek - Vybíhal, Václav - Juráček, Jaroslav - Vychytilová, Petra - Smrčka, Martin - Slabý, Ondřej
PY - 2018
TI - MicroRNA isolation and quantification in cerebrospinal fluid: A comparative methodical study
JF - Plos one
VL - 13
IS - 12
SP - 1-12
EP - 1-12
PB - Public Library of Science
SN - 19326203
KW - MicroRNA
KW - cerebrospinal fluid
N2 - Associated with the pathogenesis of many cancers, including brain tumors, microRNAs (miRNAs) present promising diagnostic biomarkers. These molecules have been also studied in cerebrospinal fluid (CSF), showing great potential as a diagnostic tool in patients with brain tumors. Even though there are some biological and technological factors that could affect the results and their biological and clinical interpretation, miRNA analysis in CSF is not fully standardized. This study aims to compare several RNA extraction and miRNA quantification approaches, including high-throughput technologies and individual miRNA detection methods, thereby contributing to the optimization and standardization of quantification of extracellular miRNAs in CSF. Such knowledge is essential for the potential use of miRNAs as diagnostic biomarkers in brain tumors.
ER -
KOPKOVÁ, Alena, Jiří ŠÁNA, Pavel FADRUS, Táňa MACHÁČKOVÁ, Marek VEČEŘA, Václav VYBÍHAL, Jaroslav JURÁČEK, Petra VYCHYTILOVÁ, Martin SMRČKA and Ondřej SLABÝ. MicroRNA isolation and quantification in cerebrospinal fluid: A comparative methodical study. \textit{Plos one}. San Francisco: Public Library of Science, 2018, vol.~13, No~12, p.~1-12. ISSN~1932-6203. Available from: https://dx.doi.org/10.1371/journal.pone.0208580.
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