Determination of lansoprazole, 5-hydroxylansoprazole, and
lansoprazole sulfone in human plasma for CYP2C19 and CYP3A4
phenotyping

J 2019

Determination of lansoprazole, 5-hydroxylansoprazole, and lansoprazole sulfone in human plasma for CYP2C19 and CYP3A4 phenotyping

KOSTOLANSKÁ, Katarína, Ondřej PEŠ, Ondřej ZENDULKA, Jan MÁCHAL, Jan JUŘICA et. al.

Basic information

Original name

Determination of lansoprazole, 5-hydroxylansoprazole, and lansoprazole sulfone in human plasma for CYP2C19 and CYP3A4 phenotyping

Authors

KOSTOLANSKÁ, Katarína (703 Slovakia, belonging to the institution), Ondřej PEŠ (203 Czech Republic, belonging to the institution), Ondřej ZENDULKA (203 Czech Republic, belonging to the institution), Jan MÁCHAL (203 Czech Republic, belonging to the institution) and Jan JUŘICA (203 Czech Republic, guarantor, belonging to the institution)

Edition

Chemical Papers, Slovenská akadémia vied, 2019, 2585-7290

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10406 Analytical chemistry

Country of publisher

Switzerland

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

URL

Impact factor

Impact factor: 1.680

RIV identification code

RIV/00216224:14110/19:00109056

Organization unit

Faculty of Medicine

DOI

http://dx.doi.org/10.1007/s11696-019-00682-4

UT WoS

000488930200009

Keywords in English

Cytochrome P450; Phenotype; Lansoprazole; 5-Hydroxylansoprazole; Lansoprazole sulfone; Liquid chromatography; Mass spectrometry; Metabolites;

Abstract

V originále

A simple and sensitive liquid chromatography method with absorbance or mass spectrometry detection was developed and validated for the determination of lansoprazole, lansoprazole sulfone, and 5-hydroxylansorpazole in human serum with omeprazole as an internal standard. Analytes were, after an extraction step with a mixture of isopropyl alcohol: dichloromethane (2:8, v/v), separated in a gradient elution (acetonitrile: 20 mM ammonium formate) and detected at 280 and 300 nm or by monitoring selective reactions in a triple quadrupole mass analyzer. The limits of detection were, for all analytes, below 2 ng/mL and 3 pg/mL in the LC UV and LC MS method, respectively. The combined linear dynamic range exceeded 5 orders of magnitude. The method was successfully applied in the assessment of CYP2C19 and CYP3A4 metabolic phenotypes in ST-elevated myocardial infarction patients receiving novel anticoagulant treatment.

Links

MUNI/A/0910/2017, interní kód MU

Name: Studium molekulární podstaty vybraných patologických stavů a onemocnění (Acronym: stumopo)

Investor: Masaryk University, Category A

MUNI/A/1132/2017, interní kód MU

Name: Behaviorální psychofarmakologie a farmakokinetika v preklinickém výzkumu léčiv

Investor: Masaryk University, Category A

ROZV/24/LF/2018, interní kód MU

Name: LF - Příspěvek na IP 2108

Investor: Ministry of Education, Youth and Sports of the CR, Internal development projects

Citovat

KOSTOLANSKÁ, Katarína, Ondřej PEŠ, Ondřej ZENDULKA, Jan MÁCHAL and Jan JUŘICA. Determination of lansoprazole, 5-hydroxylansoprazole, and lansoprazole sulfone in human plasma for CYP2C19 and CYP3A4 phenotyping. Chemical Papers. Slovenská akadémia vied, 2019, vol. 73, No 12, p. 2955-2963. ISSN 2585-7290. Available from: https://dx.doi.org/10.1007/s11696-019-00682-4.

@article{1490257,
   author = {Kostolanská, Katarína and Peš, Ondřej and Zendulka, Ondřej and Máchal, Jan and Juřica, Jan},
   article_number = {12},
   doi = {http://dx.doi.org/10.1007/s11696-019-00682-4},
   keywords = {Cytochrome P450; Phenotype; Lansoprazole; 5-Hydroxylansoprazole; Lansoprazole sulfone; Liquid chromatography; Mass spectrometry; Metabolites;},
   language = {eng},
   issn = {2585-7290},
   journal = {Chemical Papers},
   title = {Determination of lansoprazole, 5-hydroxylansoprazole, and lansoprazole sulfone in human plasma for CYP2C19 and CYP3A4 phenotyping},
   url = {https://link.springer.com/article/10.1007/s11696-019-00682-4#citeas},
   volume = {73},
   year = {2019}
}

TY  - JOUR
ID  - 1490257
AU  - Kostolanská, Katarína - Peš, Ondřej - Zendulka, Ondřej - Máchal, Jan - Juřica, Jan
PY  - 2019
TI  - Determination of lansoprazole, 5-hydroxylansoprazole, and lansoprazole sulfone in human plasma for CYP2C19 and CYP3A4 phenotyping
JF  - Chemical Papers
VL  - 73
IS  - 12
SP  - 2955-2963
EP  - 2955-2963
PB  - Slovenská akadémia vied
SN  - 25857290
KW  - Cytochrome P450
KW  - Phenotype
KW  - Lansoprazole
KW  - 5-Hydroxylansoprazole
KW  - Lansoprazole sulfone
KW  - Liquid chromatography
KW  - Mass spectrometry
KW  - Metabolites;
UR  - https://link.springer.com/article/10.1007/s11696-019-00682-4#citeas
L2  - https://link.springer.com/article/10.1007/s11696-019-00682-4#citeas
N2  - A simple and sensitive liquid chromatography method with absorbance or mass spectrometry detection was developed and validated for the determination of lansoprazole, lansoprazole sulfone, and 5-hydroxylansorpazole in human serum with omeprazole as an internal standard. Analytes were, after an extraction step with a mixture of isopropyl alcohol: dichloromethane (2:8, v/v), separated in a gradient elution (acetonitrile: 20 mM ammonium formate) and detected at 280 and 300 nm or by monitoring selective reactions in a triple quadrupole mass analyzer. The limits of detection were, for all analytes, below 2 ng/mL and 3 pg/mL in the LC UV and LC MS method, respectively. The combined linear dynamic range exceeded 5 orders of magnitude. The method was successfully applied in the assessment of CYP2C19 and CYP3A4 metabolic phenotypes in ST-elevated myocardial infarction patients receiving novel anticoagulant treatment.
ER  -

KOSTOLANSKÁ, Katarína, Ondřej PEŠ, Ondřej ZENDULKA, Jan MÁCHAL and Jan JUŘICA. Determination of lansoprazole, 5-hydroxylansoprazole, and lansoprazole sulfone in human plasma for CYP2C19 and CYP3A4 phenotyping. \textit{Chemical Papers}. Slovenská akadémia vied, 2019, vol.~73, No~12, p.~2955-2963. ISSN~2585-7290. Available from: https://dx.doi.org/10.1007/s11696-019-00682-4.

Detailed Information on Publication Record